##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920930_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4396224
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.115132895867	31.0	31.0	34.0	28.0	34.0
2	31.260372082951186	33.0	31.0	34.0	28.0	34.0
3	31.343359437553683	33.0	31.0	34.0	28.0	34.0
4	34.86913360192747	37.0	35.0	37.0	32.0	37.0
5	34.57157005648484	37.0	35.0	37.0	31.0	37.0
6	34.30456273383704	37.0	35.0	37.0	30.0	37.0
7	34.20080869400649	37.0	35.0	37.0	30.0	37.0
8	34.195528935741216	37.0	35.0	37.0	30.0	37.0
9	35.75569920913948	39.0	37.0	39.0	30.0	39.0
10-11	35.71730216658659	39.0	36.0	39.0	30.0	39.0
12-13	35.62005200371955	39.0	35.0	39.0	30.0	39.0
14-15	36.84415330065074	40.0	37.0	41.0	30.0	41.0
16-17	36.845801533315864	40.0	37.0	41.0	30.0	41.0
18-19	36.877660578714824	40.0	37.0	41.0	30.0	41.0
20-21	36.86835657145768	40.0	37.0	41.0	30.0	41.0
22-23	36.81710554330262	40.0	37.0	41.0	30.0	41.0
24-25	36.7993458704561	40.0	37.0	41.0	30.0	41.0
26-27	36.67755510183285	40.0	37.0	41.0	30.0	41.0
28-29	36.579072517687905	40.0	36.5	41.0	30.0	41.0
30-31	36.44024019249247	40.0	36.0	41.0	29.0	41.0
32-33	36.29896088097422	40.0	36.0	41.0	29.0	41.0
34-35	36.18482554574108	40.0	36.0	41.0	28.5	41.0
36-37	36.05631958244166	40.0	35.5	41.0	28.0	41.0
38-39	35.932110147253645	40.0	35.0	41.0	27.0	41.0
40-41	35.80271046243322	40.0	35.0	41.0	27.0	41.0
42-43	35.6592108363905	40.0	35.0	41.0	26.5	41.0
44-45	35.46747322247456	40.0	35.0	41.0	25.5	41.0
46-47	35.26672150918607	39.5	35.0	41.0	24.5	41.0
48-49	35.02185853132143	39.0	35.0	41.0	24.0	41.0
50-51	34.84168550101178	39.0	35.0	41.0	23.5	41.0
52-53	34.651510250615075	39.0	34.0	41.0	23.0	41.0
54-55	34.45092765973708	39.0	34.0	41.0	22.5	41.0
56-57	34.204107320282134	38.0	34.0	41.0	22.0	41.0
58-59	33.98134876657787	38.0	34.0	40.0	21.0	41.0
60-61	33.724902780204104	37.5	33.0	40.0	20.0	41.0
62-63	33.434321704262565	37.0	33.0	40.0	19.5	41.0
64-65	33.134077676660695	36.5	33.0	40.0	18.0	41.0
66-67	32.812322802477766	36.0	32.5	39.0	17.5	41.0
68-69	32.45261797397039	35.5	32.0	39.0	15.5	41.0
70-71	32.11991950364677	35.0	32.0	39.0	13.0	40.5
72-73	31.753435334505248	35.0	32.0	37.5	11.5	40.0
74-75	31.421764564317016	35.0	31.5	37.0	9.0	39.0
76-77	31.201571280262336	35.0	31.5	37.0	8.0	39.0
78-79	31.044039839644206	35.0	32.0	36.0	7.5	39.0
80-81	30.787508893996304	35.0	31.0	36.0	7.0	37.0
82-83	30.562171763768177	35.0	31.0	35.5	6.0	37.0
84-85	30.3176265586103	35.0	31.0	35.0	2.0	36.5
86-87	30.146711359566755	35.0	31.0	35.0	2.0	36.0
88-89	29.995696420382583	35.0	31.0	35.0	2.0	36.0
90-91	29.835263285037342	35.0	31.0	35.0	2.0	36.0
92-93	29.718396173625365	35.0	31.0	35.0	2.0	35.0
94-95	29.62797505313651	35.0	31.0	35.0	2.0	35.0
96-97	29.55940461632528	35.0	31.0	35.0	2.0	35.0
98-99	29.45707009015009	34.5	31.0	35.0	2.0	35.0
100-101	29.362256336346828	34.0	30.0	35.0	2.0	35.0
102-103	27.953120109439375	32.5	28.0	34.5	2.0	35.0
104-105	29.023923257777582	34.0	29.5	35.0	2.0	35.0
106-107	29.203086671652763	34.0	30.0	35.0	2.0	35.0
108-109	29.301875086437818	34.5	30.5	35.0	2.0	35.0
110-111	29.48338835782708	35.0	31.0	35.0	2.0	35.0
112-113	29.464987339134677	35.0	31.0	35.0	2.0	35.0
114-115	29.386044250702422	35.0	31.0	35.0	2.0	35.0
116-117	29.276330550945538	35.0	31.0	35.0	2.0	35.0
118-119	29.178117971240773	35.0	31.0	35.0	2.0	35.0
120-121	29.003151227053035	35.0	31.0	35.0	2.0	35.0
122-123	28.82365275290795	35.0	31.0	35.0	2.0	35.0
124-125	28.08511156392395	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87740.0
3	33864.0
4	13974.0
5	11578.0
6	13138.0
7	15508.0
8	17028.0
9	18694.0
10	21140.0
11	24066.0
12	25405.0
13	28507.0
14	34359.0
15	31016.0
16	20184.0
17	17630.0
18	16830.0
19	16180.0
20	16032.0
21	16185.0
22	17528.0
23	20220.0
24	26866.0
25	35489.0
26	44410.0
27	53609.0
28	64635.0
29	77334.0
30	92879.0
31	113399.0
32	140908.0
33	181357.0
34	244463.0
35	366719.0
36	683950.0
37	1238475.0
38	513785.0
39	1140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92557293541393	18.51078394921435	12.71904879032341	25.84459432504831
2	16.28314916121612	20.334116792784528	38.163210709645014	25.21952333635434
3	19.18976788346837	23.9672152999513	29.42486426336217	27.418152553218157
4	13.307750138474539	16.282990777083857	35.950310672036565	34.45894841240504
5	13.91191728717374	37.200556202596886	34.499677187589064	14.387849322640307
6	33.2651695207845	36.211666959122425	16.520723278363032	14.002440241730046
7	28.459761118436244	31.79161172815419	21.16646739012961	18.58215976327995
8	26.2582708535621	35.241958013957735	19.745784151083505	18.753986981396665
9	26.537039762801374	15.182131570799346	19.374671407423854	38.906157258975426
10-11	25.420217700028036	25.585196079602294	27.75178852647258	21.242797693897085
12-13	26.021017477026888	23.037632283445312	27.777490244566906	23.1638599949609
14-15	23.258005780274488	24.52248348403622	25.254402835032842	26.965107900656456
16-17	23.191832145251812	27.305712779519205	26.11106345790992	23.39139161731906
18-19	23.077470000871404	26.373079699700426	27.870925878188785	22.67852442123939
20-21	23.913520992916723	25.623055608419033	27.640041410585315	22.823381988078932
22-23	23.425857044382717	25.53790924167867	27.3955041711882	23.640729542750414
24-25	23.19407455872395	25.7414451134458	27.63270699625167	23.431773331578583
26-27	23.23774379245819	25.874580543831087	27.62273497081196	23.264940692898765
28-29	23.118106560406332	25.93390988460216	27.703296429547642	23.244687125443868
30-31	23.417043941472034	25.798817456473582	27.522186624941792	23.261951977112588
32-33	23.01051564720797	25.91359412866411	27.545774545752366	23.530115678375555
34-35	23.32623550113543	25.88818929291691	27.587562523127485	23.198012682820178
36-37	23.475395633499332	25.75375635405862	27.394521432427716	23.37632658001433
38-39	23.348968333436854	25.940062344335395	27.362922434518765	23.348046887708986
40-41	23.471950256852985	25.900225438166498	27.40559997187853	23.222224333101984
42-43	23.428445775055735	25.78602880140441	27.379808438842662	23.4057169846972
44-45	23.41254786697273	25.898359972830594	27.22972245090276	23.45936970929392
46-47	23.424276282007092	25.896887946083545	27.048434576839885	23.630401195069474
48-49	23.444276732736284	26.023518239757365	26.906899753710633	23.625305273795718
50-51	23.371677450771962	26.167878051396748	26.818894257527916	23.641550240303378
52-53	23.382892790710187	26.20170660099031	26.742132902032704	23.6732677062668
54-55	23.352287812627758	26.148683989304377	26.718444534487197	23.780583663580668
56-57	23.363958442919987	26.205343248157583	26.539210939675616	23.891487369246814
58-59	23.1389668700845	26.102541742706155	26.807631541438642	23.950859845770704
60-61	23.320682505398587	26.127946840358746	26.805194426992347	23.746176227250324
62-63	23.06713949432074	26.166771859140077	26.795321536304602	23.970767110234583
64-65	23.049991212448862	26.004428470755652	26.867041791773143	24.07853852502234
66-67	23.049632481337394	26.200086592752648	26.680396524732057	24.069884401177898
68-69	23.248331241638574	25.978666874755508	26.635090853642374	24.13791102996354
70-71	22.95977495267909	25.8002352395166	26.8551843695399	24.384805438264415
72-73	23.090451835418925	25.785087375521748	26.75910548233861	24.365355306720712
74-75	23.004222960963915	25.941890919638617	26.63646988900864	24.417416230388824
76-77	22.93769235572915	25.71143680324101	26.81584320186402	24.53502763916582
78-79	22.99881652335421	25.734599560825966	26.724583917639855	24.541999998179968
80-81	22.803665026880942	25.765221330121555	26.72653556722761	24.704578075769884
82-83	22.74947528099141	25.785679413861683	26.72606733056001	24.738777974586903
84-85	22.756842698332946	25.8288777413934	26.549056405672477	24.865223154601182
86-87	22.678805170618656	25.660259227100873	26.6942055031641	24.966730099116376
88-89	22.692132466904603	25.57858006171172	26.648484934662363	25.080802536721315
90-91	22.615228779767303	25.591734569856726	26.623502854806137	25.169533795569834
92-93	22.492179563434174	25.57310340834651	26.696179636687674	25.238537391531647
94-95	22.3758430379407	25.445683564981675	26.774751910161797	25.403721486915824
96-97	22.415254396345084	25.410980929502074	26.68160050086319	25.492164173289655
98-99	22.359128134697734	25.40685978207235	26.59663046368945	25.637381619540463
100-101	22.24591319367187	25.382153418302323	26.683795603034564	25.688137784991238
102-103	22.15925734767058	25.29156962467972	26.71639038732878	25.83278264032092
104-105	22.04623107027201	25.38640297832308	26.584395242244668	25.982970709160234
106-107	21.961980928133244	25.386832136043164	26.58283322706004	26.068353708763553
108-109	21.873617224865143	25.421677455109077	26.62232155389971	26.08238376612607
110-111	21.799842211298547	25.450218756654152	26.531034962845943	26.218904069201354
112-113	21.678824221110638	25.53303684756439	26.57701572161686	26.211123209708116
114-115	21.602891289450262	25.584340175786302	26.504946300156107	26.307822234607325
116-117	21.495383015347183	25.492550018186776	26.568060743608296	26.44400622285774
118-119	21.4566293956148	25.462051439124224	26.57058363967295	26.510735525588025
120-121	21.383501801412127	25.575866707444916	26.506184012340846	26.534447478802115
122-123	21.278855693411835	25.616591462853584	26.42708206210209	26.67747078163249
124-125	21.137703738177873	25.61724814533259	26.409829293713717	26.83521882277582
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	82.0
2	181.0
3	278.5
4	211.0
5	150.5
6	253.0
7	571.0
8	934.5
9	1037.5
10	919.0
11	828.5
12	906.0
13	1068.0
14	1257.5
15	1418.5
16	1663.5
17	1978.0
18	2267.5
19	2611.0
20	3070.5
21	3666.0
22	4522.5
23	5704.0
24	7230.0
25	9368.0
26	12801.5
27	17462.5
28	21996.5
29	27238.0
30	33276.5
31	40116.5
32	49212.0
33	57444.0
34	64962.5
35	72944.0
36	82771.5
37	92799.5
38	100696.0
39	108725.0
40	118370.0
41	126804.5
42	134168.0
43	144979.5
44	155214.5
45	164198.5
46	171288.5
47	177265.5
48	184791.5
49	190730.5
50	193677.5
51	188704.5
52	178994.5
53	172991.0
54	166911.0
55	158224.5
56	146050.0
57	131646.0
58	118853.5
59	106112.5
60	91463.5
61	76603.0
62	62329.0
63	47096.0
64	34434.0
65	26384.0
66	20206.5
67	15298.5
68	11864.0
69	9541.5
70	7956.0
71	6560.0
72	5227.5
73	4332.0
74	3496.0
75	2714.5
76	2030.0
77	1359.5
78	894.0
79	549.0
80	356.5
81	267.0
82	174.0
83	118.0
84	100.0
85	62.0
86	37.0
87	28.5
88	21.0
89	16.0
90	12.0
91	9.0
92	7.0
93	6.0
94	6.5
95	13.5
96	13.5
97	4.5
98	2.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2154803758862151
2	0.007642922653622745
3	6.141634275232564E-4
4	0.0024794005037049975
5	0.0814335211308614
6	0.05452406428789797
7	0.026363533796276078
8	0.015194858132797601
9	0.013261380675779942
10-11	0.011293783028344323
12-13	0.01356846238954157
14-15	0.021177264852746357
16-17	0.02372490573728727
18-19	0.023042501928928098
20-21	0.01659378593993391
22-23	0.01351159540551164
24-25	0.012158161185599278
26-27	0.01143026379001616
28-29	0.01375043673843735
30-31	0.010122323157327744
32-33	0.014364600165960606
34-35	0.017071468605785328
36-37	0.018311168857637828
38-39	0.02176868148665764
40-41	0.022871900976838305
42-43	0.020870183138984727
44-45	0.019095933237250878
46-47	0.01890258549154911
48-49	0.01784485958859239
50-51	0.014432840546796524
52-53	0.011998933630315472
54-55	0.017765245810950486
56-57	0.02092705012301466
58-59	0.01718520257384519
60-61	0.019550869109490325
62-63	0.02515795373484154
64-65	0.018231555079995927
66-67	0.021313745614418192
68-69	0.01513799114876767
70-71	0.016013742702828612
72-73	0.02339507722991367
74-75	0.013909664293721157
76-77	0.01272683102589859
78-79	0.01592275552838072
80-81	0.016627906130351866
82-83	0.012237774963241181
84-85	0.016320824416590236
86-87	0.016707519907993768
88-89	0.017890353175816335
90-91	0.012886058581182398
92-93	0.012044427217539417
94-95	0.013386488040645789
96-97	0.013932411087333129
98-99	0.012397002518524988
100-101	0.016787133685635672
102-103	0.022849154183226333
104-105	0.007449574907920979
106-107	0.007392707923891049
108-109	0.014216746007482786
110-111	0.010736486584851
112-113	0.009041850460759051
114-115	0.005948286529530797
116-117	0.003935195294871235
118-119	0.0021723187899433697
120-121	0.0020358380282715347
122-123	0.0013534342199123613
124-125	4.0944228501550424E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4396224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.442300347146976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.33111551822475	31.734541899529894
2	14.67087028681987	13.626979284290455
3	6.281964668715141	8.752466697439026
4	3.3752765101044635	6.2702242174776615
5	1.9347523688443626	4.4927175305612
6	1.2498054885017622	3.482630512350695
7	0.8502607745898019	2.764164638682819
8	0.5857356898725817	2.1762330266484624
9	0.4470206870934272	1.8684602110242858
>10	2.1182832122055295	17.01519325556977
>50	0.11349646851086329	3.578471157085292
>100	0.03970564305533284	3.1678190945174247
>500	0.0011744116403149339	0.3630704086623928
>1k	4.4040421789744405E-4	0.4290211125641057
>5k	9.786760397716122E-5	0.27800695359653727
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6821	0.15515587922726415	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5412	0.12310564702799494	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.2746793611972456E-5	0.0
3	0.0	0.0	0.0	2.2746793611972456E-5	0.0
4	0.0	0.0	0.0	2.2746793611972456E-5	0.0
5	0.0	0.0	0.0	2.2746793611972456E-5	0.0
6	0.0	0.0	0.0	2.2746793611972456E-5	0.0
7	0.0	0.0	0.0	2.2746793611972456E-5	0.0
8	0.0	0.0	0.0	2.2746793611972456E-5	0.0
9	0.0	0.0	0.0	2.2746793611972456E-5	0.0
10-11	0.0	0.0	0.0	2.2746793611972456E-5	0.0
12-13	0.0	0.0	0.0	2.2746793611972456E-5	0.0
14-15	0.0	0.0	0.0	2.2746793611972456E-5	0.0
16-17	0.0	1.1373396805986228E-5	0.0	2.2746793611972456E-5	0.0
18-19	0.0	2.2746793611972456E-5	0.0	7.96137776419036E-5	0.0
20-21	0.0	2.2746793611972456E-5	0.0	1.3648076167183473E-4	0.0
22-23	0.0	2.2746793611972456E-5	0.0	1.8197434889577967E-4	0.0
24-25	0.0	2.2746793611972456E-5	0.0	3.639486977915593E-4	0.0
26-27	0.0	2.2746793611972456E-5	0.0	7.506441891950911E-4	0.0
28-29	0.0	2.2746793611972456E-5	0.0	0.002524894090928943	0.0
30-31	0.0	2.2746793611972456E-5	0.0	0.009599146904252377	0.0
32-33	0.0	2.2746793611972456E-5	0.0	0.028638213157473322	0.0
34-35	0.0	2.2746793611972456E-5	0.0	0.061268488593847814	0.0
36-37	0.0	2.2746793611972456E-5	0.0	0.12087646125402163	0.0
38-39	0.0	2.2746793611972456E-5	0.0	0.2198363868629078	0.0
40-41	0.0	2.2746793611972456E-5	0.0	0.3384609155493442	0.0
42-43	0.0	2.2746793611972456E-5	0.0	0.45206977624434064	0.0
44-45	0.0	2.2746793611972456E-5	0.0	0.5899153455328937	0.0
46-47	0.0	2.2746793611972456E-5	0.0	0.761346555589524	0.0
48-49	0.0	2.2746793611972456E-5	0.0	0.9423996593440189	0.0
50-51	0.0	2.2746793611972456E-5	0.0	1.127285597822131	0.0
52-53	0.0	2.2746793611972456E-5	0.0	1.3151741130570236	0.0
54-55	0.0	2.2746793611972456E-5	0.0	1.5281068480586977	0.0
56-57	0.0	2.2746793611972456E-5	0.0	1.7825069878149975	0.0
58-59	0.0	2.2746793611972456E-5	0.0	2.0526706555443943	0.0
60-61	0.0	2.2746793611972456E-5	0.0	2.3377334730896333	0.0
62-63	0.0	2.2746793611972456E-5	0.0	2.6274707567221323	0.0
64-65	0.0	2.2746793611972456E-5	0.0	2.939897966982574	0.0
66-67	0.0	2.2746793611972456E-5	0.0	3.306564906610764	0.0
68-69	0.0	2.2746793611972456E-5	0.0	3.7119468889665312	0.0
70-71	0.0	2.2746793611972456E-5	0.0	4.150834898312734	0.0
72-73	0.0	2.2746793611972456E-5	0.0	4.58826711286777	0.0
74-75	0.0	2.2746793611972456E-5	0.0	5.034809418264402	0.0
76-77	0.0	2.2746793611972456E-5	0.0	5.542836306794195	0.0
78-79	0.0	2.2746793611972456E-5	0.0	6.102384682855105	0.0
80-81	0.0	2.2746793611972456E-5	0.0	6.684600238750345	0.0
82-83	0.0	2.2746793611972456E-5	0.0	7.289119025782126	0.0
84-85	0.0	2.2746793611972456E-5	0.0	7.901098761118632	0.0
86-87	0.0	2.2746793611972456E-5	0.0	8.55090641423185	0.0
88-89	0.0	2.2746793611972456E-5	0.0	9.265360454790294	0.0
90-91	0.0	2.2746793611972456E-5	0.0	10.014855930907979	0.0
92-93	2.2746793611972456E-5	2.2746793611972456E-5	0.0	10.77450785037341	0.0
94-95	2.2746793611972456E-5	2.2746793611972456E-5	0.0	11.540904194144794	0.0
96-97	2.2746793611972456E-5	2.2746793611972456E-5	0.0	12.333436603776331	0.0
98-99	2.2746793611972456E-5	2.2746793611972456E-5	0.0	13.18501968962455	0.0
100-101	2.2746793611972456E-5	2.2746793611972456E-5	0.0	14.100771480252142	0.0
102-103	2.2746793611972456E-5	2.2746793611972456E-5	0.0	15.02363164388348	0.0
104-105	2.2746793611972456E-5	2.2746793611972456E-5	0.0	15.941669487269074	0.0
106-107	2.2746793611972456E-5	2.2746793611972456E-5	0.0	16.871774504665822	0.0
108-109	2.2746793611972456E-5	2.2746793611972456E-5	0.0	17.8488630242681	0.0
110-111	2.2746793611972456E-5	2.2746793611972456E-5	0.0	18.87280993871104	0.0
112-113	2.2746793611972456E-5	2.2746793611972456E-5	0.0	19.931036271127223	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	12830	0.0	36.69294	1
GGTATCA	8810	0.0	34.946686	1
TCAACGC	15280	0.0	30.20191	4
ATCAACG	15545	0.0	29.610437	3
CAACGCA	15560	0.0	29.505358	5
TATCAAC	16070	0.0	29.014284	2
AACGCAG	16160	0.0	28.578157	6
GTACATG	15975	0.0	26.182392	1
CATGGGG	9470	0.0	25.717539	4
TACATGG	16325	0.0	25.27819	2
ACGCAGA	18325	0.0	25.066475	7
ACATGGG	16605	0.0	24.60035	3
CGCAGAG	18970	0.0	24.14871	8
GCAGAGT	20915	0.0	21.391459	9
GAGTACT	10825	0.0	21.161568	12-13
AGTACTT	11490	0.0	20.920708	12-13
AGAGTAC	18380	0.0	20.588474	10-11
GTACTTT	11785	0.0	19.437868	14-15
CAGAGTA	20020	0.0	18.530405	10-11
ATGGGGG	5035	0.0	17.503235	5
>>END_MODULE