FastQCFastQC Report
Mon 15 Aug 2016
SRR2920929_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920929_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3937053
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG158250.031.2898461
GTATCAA99850.031.0910781
TACATGG157550.029.9409082
GGTATCA74200.028.5885561
ACATGGG161950.028.4647393
CATGGGG96300.028.2642274
TCAACGC111050.026.6017554
AACGCAG111100.026.5182936
TATCAAC116850.025.995592
CAACGCA113650.025.8883695
ATCAACG117900.025.2582573
ACGCAGA124350.023.4033957
CGCAGAG129000.022.4188148
ATGGGGA54300.021.0595255
GCAGAGT145850.018.9304569
ATGGGGG53350.018.8668545
AGAGTAC125450.017.21701610-11
CAGAGTA135950.016.98143810-11
GTGTAGC26900.016.613271
GTGTAAG18300.016.28041
Produced by FastQC (version 0.11.5)