FastQCFastQC Report
Mon 15 Aug 2016
SRR2920929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3937053
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79120.20096249656786436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65290.16583469920267774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45080.11450188757936458No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT39670.10076064508148608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86550.042.1171531
GTATCAA131600.040.302381
TCAACGC158350.033.055464
CAACGCA160500.032.5384565
ATCAACG162800.032.1519173
AACGCAG165250.031.8019876
TATCAAC167900.031.3175582
CATGGGG89400.028.8418274
ACGCAGA187700.027.8330277
TACATGG154200.027.4962522
GTACATG156300.027.3299541
CGCAGAG193400.026.8561538
ACATGGG154500.026.4789453
GAGTACT114300.023.3472312-13
GCAGAGT217300.022.945529
AGAGTAC192400.021.56999210-11
AGTACTT125150.021.39443612-13
GTACTTT129600.020.56800314-15
CAGAGTA202950.020.31678810-11
ATGGGGG53350.019.9797945
Produced by FastQC (version 0.11.5)