##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920929_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3937053
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.993451447059513	31.0	31.0	34.0	27.0	34.0
2	31.0822313034648	31.0	31.0	34.0	27.0	34.0
3	31.17532276045052	33.0	31.0	34.0	27.0	34.0
4	34.71515217092582	37.0	35.0	37.0	32.0	37.0
5	34.40372278452944	37.0	35.0	37.0	30.0	37.0
6	34.10375806472506	37.0	35.0	37.0	30.0	37.0
7	33.988650394089184	37.0	35.0	37.0	30.0	37.0
8	33.972288917624425	37.0	35.0	37.0	30.0	37.0
9	35.49992291188359	39.0	35.0	39.0	30.0	39.0
10-11	35.47203111057941	39.0	35.0	39.0	30.0	39.0
12-13	35.35466807279455	39.0	35.0	39.0	29.0	39.0
14-15	36.54099487611673	40.0	36.0	41.0	29.0	41.0
16-17	36.5415062992548	40.0	36.0	41.0	29.0	41.0
18-19	36.58472555487569	40.0	36.5	41.0	29.0	41.0
20-21	36.58332984087336	40.0	37.0	41.0	29.0	41.0
22-23	36.53765976734375	40.0	36.5	41.0	29.0	41.0
24-25	36.51960755925816	40.0	36.5	41.0	29.0	41.0
26-27	36.38917154531575	40.0	36.0	41.0	29.0	41.0
28-29	36.284544429551744	40.0	36.0	41.0	28.5	41.0
30-31	36.13152159749945	40.0	36.0	41.0	28.0	41.0
32-33	35.969105953107565	40.0	35.5	41.0	27.0	41.0
34-35	35.83948984176743	40.0	35.0	41.0	27.0	41.0
36-37	35.70246882630231	40.0	35.0	41.0	26.5	41.0
38-39	35.57740269181035	40.0	35.0	41.0	26.0	41.0
40-41	35.43725001924028	40.0	35.0	41.0	25.5	41.0
42-43	35.285166468421934	40.0	35.0	41.0	25.0	41.0
44-45	35.08092753640858	39.0	35.0	41.0	24.0	41.0
46-47	34.86133473438127	39.0	35.0	41.0	23.5	41.0
48-49	34.59123499226452	39.0	34.0	41.0	22.0	41.0
50-51	34.40811883914186	39.0	34.0	41.0	20.5	41.0
52-53	34.22064511196572	39.0	34.0	41.0	19.5	41.0
54-55	34.019139950617884	38.5	34.0	41.0	18.5	41.0
56-57	33.77657615988406	38.0	33.0	40.5	17.5	41.0
58-59	33.555269258503756	38.0	33.0	40.0	14.5	41.0
60-61	33.299648747426055	37.5	33.0	40.0	13.0	41.0
62-63	33.01169377196599	37.0	33.0	40.0	12.0	41.0
64-65	32.707035185962695	36.5	32.0	40.0	8.0	41.0
66-67	32.38712102681879	36.0	32.0	39.0	7.5	41.0
68-69	32.01300490493778	35.5	32.0	39.0	6.5	41.0
70-71	31.673420576253356	35.0	31.5	38.5	2.0	40.0
72-73	31.306944305804365	35.0	31.0	37.5	2.0	40.0
74-75	30.971798195249086	35.0	31.0	37.0	2.0	39.0
76-77	30.755245103380624	35.0	31.0	37.0	2.0	39.0
78-79	30.601437928318468	35.0	31.0	36.0	2.0	39.0
80-81	30.339643256008998	35.0	31.0	36.0	2.0	37.5
82-83	30.105401425888857	35.0	31.0	35.5	2.0	37.0
84-85	29.855351705958746	35.0	31.0	35.0	2.0	37.0
86-87	29.67789740193998	35.0	31.0	35.0	2.0	36.0
88-89	29.513885385845708	35.0	30.0	35.0	2.0	36.0
90-91	29.34601642395975	35.0	30.0	35.0	2.0	36.0
92-93	29.21817841923896	34.0	30.0	35.0	2.0	35.0
94-95	29.120641124211435	34.0	30.0	35.0	2.0	35.0
96-97	29.047281050064605	34.0	30.0	35.0	2.0	35.0
98-99	28.937370032864685	34.0	30.0	35.0	2.0	35.0
100-101	28.837475517855616	34.0	29.5	35.0	2.0	35.0
102-103	27.419453332225906	32.5	26.0	34.0	2.0	35.0
104-105	28.483615790795806	34.0	29.0	35.0	2.0	35.0
106-107	28.658559968585642	34.0	29.0	35.0	2.0	35.0
108-109	28.749246454137143	34.0	29.5	35.0	2.0	35.0
110-111	28.91316004635955	35.0	30.0	35.0	2.0	35.0
112-113	28.874184320099324	35.0	30.0	35.0	2.0	35.0
114-115	28.770191308067226	35.0	30.0	35.0	2.0	35.0
116-117	28.634749138505377	35.0	30.0	35.0	2.0	35.0
118-119	28.511806292676276	35.0	30.0	35.0	2.0	35.0
120-121	28.307929941507012	35.0	30.0	35.0	2.0	35.0
122-123	28.092151159763407	35.0	29.5	35.0	2.0	35.0
124-125	27.325806129610143	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87465.0
3	33555.0
4	13699.0
5	11637.0
6	13461.0
7	15736.0
8	17523.0
9	19032.0
10	21381.0
11	24318.0
12	25822.0
13	29282.0
14	35428.0
15	31849.0
16	20182.0
17	17623.0
18	16693.0
19	16206.0
20	16201.0
21	16605.0
22	17366.0
23	19424.0
24	25441.0
25	33308.0
26	41617.0
27	49407.0
28	60065.0
29	71499.0
30	85802.0
31	104748.0
32	130577.0
33	165630.0
34	223346.0
35	325975.0
36	594332.0
37	1058972.0
38	444759.0
39	1087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.018505240432205	18.996283578180396	12.623133039552492	25.362078141834903
2	16.240952580778398	20.95726991600703	37.984072173092684	24.817705330121893
3	18.816477810165434	24.801881012784264	30.198286626358765	26.183354550691533
4	13.132320675362703	16.84792299450897	36.227338513972434	33.7924178161559
5	13.405014859719216	37.762447350378984	34.74388178928763	14.088656000614167
6	32.38017182117485	36.76297140591704	16.83750941890592	14.019347354002193
7	27.445712306921127	32.71041999013725	21.64587028372054	18.19799741922109
8	26.147878208979492	35.25090723412039	20.17663279913322	18.4245817577669
9	26.380396774287785	15.022145334872775	19.782039606299513	38.81541828453992
10-11	25.07100397286931	26.00902738941499	28.153988155441194	20.765980482274504
12-13	25.67070642022963	23.57902732184217	28.147028993376733	22.603237264551463
14-15	23.03724005333073	24.98807222818399	25.66574496366031	26.308942754824965
16-17	22.69763677063853	27.817937413635896	26.53767170880189	22.946754106923688
18-19	22.659492791658007	26.714461448721003	28.504314131581904	22.121731628039086
20-21	23.51039756730649	25.96711062391755	28.300499634498976	22.221992174276984
22-23	23.13980336234488	25.88321949640783	28.064648636509936	22.912328504737356
24-25	22.793785727658552	26.12149569508943	28.272464906462535	22.81225367078948
26-27	22.752956053511383	26.18599684040702	28.280821209663305	22.78022589641829
28-29	22.748727578537277	26.25951974226789	28.379936392581158	22.611816286613674
30-31	22.992455976259873	26.096941898976432	28.206426010497314	22.70417611426638
32-33	22.645286406639865	26.262464860613555	28.218864331611144	22.873384401135436
34-35	22.891182294498837	26.192278980687355	28.27227245689131	22.644266267922497
36-37	23.044581149072542	26.000945048719547	28.220907678647865	22.73356612356004
38-39	22.85772573101993	26.163077912326514	28.126526312500133	22.852670044153424
40-41	22.915978859905913	26.070987373995603	28.163046246655178	22.84998751944331
42-43	22.962682847391626	26.080367744980826	28.052114142400068	22.90483526522748
44-45	22.935256572455792	26.148814259975673	27.93587136674359	22.980057800824945
46-47	23.01447333556387	26.160469560927304	27.635011595994484	23.19004550751434
48-49	22.913931191850057	26.2959085023924	27.547610122801196	23.242550182956347
50-51	22.743053504751156	26.476798273374058	27.44153476845397	23.338613453420816
52-53	22.86472597976686	26.439597095079044	27.369406015884675	23.32627090926942
54-55	22.691214719076175	26.39521951135244	27.33976588368772	23.573799885883666
56-57	22.699997395970613	26.49193417775867	27.108682659641293	23.699385766629426
58-59	22.505930284621904	26.365711999339492	27.41558990057445	23.71276781546415
60-61	22.677967884333196	26.426375006605234	27.35942790538865	23.536229203672917
62-63	22.459363458451122	26.46534559638582	27.343522733826365	23.731768211336693
64-65	22.281355317329545	26.4258629511132	27.342214221513604	23.950567510043648
66-67	22.44997246715271	26.42704938700513	27.16254160889638	23.960436536945778
68-69	22.632524432960842	26.226309621054284	27.12922061830319	24.011945327681687
70-71	22.351725526384143	26.042095489351645	27.27488198312802	24.331297001136186
72-73	22.485100441260926	26.00214196440061	27.175496902840408	24.33726069149806
74-75	22.299918569350755	26.08216963461124	27.08538862844037	24.53252316759764
76-77	22.28902670347405	25.97740293542865	27.15207991608851	24.58149044500879
78-79	22.300099011347246	26.009309225964977	27.07245865053694	24.618133112150833
80-81	22.26157157660405	26.010195633513035	26.98285053491891	24.745382254964003
82-83	22.191488896882348	25.94410031926417	27.07615700237162	24.788253781481863
84-85	22.165814281257717	25.84418418434693	26.870603220604387	25.119398313790963
86-87	21.987831443904607	25.774272014226284	27.106728906671744	25.13116763519736
88-89	21.990431978526253	25.730211669897034	27.011325109876772	25.26803124169994
90-91	21.950443480060066	25.700936775497134	27.022860583881435	25.32575916056136
92-93	21.96283203465359	25.568554489933092	26.98459975989201	25.484013715521307
94-95	21.76148033983983	25.53141412495334	27.071855554989625	25.635249980217207
96-97	21.775144020795135	25.370091051753498	27.093526669329776	25.761238258121594
98-99	21.726117442399836	25.325489377203397	26.942012948446525	26.00638023195024
100-101	21.65394557175066	25.407868914928073	27.021815756882916	25.916369756438346
102-103	21.528987191750282	25.230968958127708	27.093913191493684	26.146130658628326
104-105	21.43931360666275	25.36823825430688	26.855492402809777	26.336955736220585
106-107	21.282240883494	25.495582250561572	26.94047322995997	26.281703635984456
108-109	21.172278567818555	25.52340986678295	26.906234912721942	26.398076652676554
110-111	21.144227580463266	25.668772844142318	26.772137694141474	26.414861881252943
112-113	21.024380361841125	25.773556220386634	26.775900205058967	26.426163212713277
114-115	20.894599788483003	25.83513587623652	26.696647873482	26.573616461798473
116-117	20.813985357473882	25.777815935374353	26.768344243386082	26.639854463765676
118-119	20.748152447380154	25.734048943793	26.72786016639721	26.78993844242964
120-121	20.752936562577432	25.888474567359548	26.65390046656575	26.70468840349727
122-123	20.592787891257025	25.952047122293937	26.532833459063905	26.922331527385136
124-125	20.46585588690773	26.04330469162946	26.485922266844092	27.004917154618717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	178.0
1	163.0
2	290.0
3	478.5
4	373.0
5	245.0
6	406.5
7	853.5
8	1414.5
9	1602.5
10	1336.5
11	1110.5
12	1165.0
13	1321.0
14	1498.0
15	1689.0
16	1949.5
17	2262.0
18	2608.5
19	2993.0
20	3456.5
21	4147.0
22	5106.5
23	6457.0
24	8270.5
25	10624.5
26	14460.0
27	19480.0
28	23941.5
29	29490.0
30	36255.5
31	43368.5
32	51539.5
33	58605.5
34	66368.0
35	74773.5
36	84048.0
37	92855.0
38	99065.0
39	104433.5
40	110387.0
41	116874.5
42	122039.0
43	129601.5
44	137902.5
45	144609.0
46	150742.5
47	154589.0
48	158208.0
49	161872.5
50	164845.5
51	162480.0
52	154987.0
53	149913.5
54	145323.0
55	137504.0
56	127632.5
57	116006.0
58	103079.5
59	90441.5
60	76526.0
61	62074.5
62	49100.0
63	36632.0
64	26712.5
65	20682.5
66	15834.0
67	11995.0
68	9192.0
69	6926.5
70	5358.5
71	4154.5
72	3128.0
73	2437.5
74	1962.5
75	1491.5
76	1078.5
77	722.5
78	455.5
79	285.5
80	189.5
81	131.0
82	91.0
83	66.0
84	43.5
85	23.0
86	20.5
87	21.0
88	9.0
89	8.5
90	10.0
91	6.0
92	4.0
93	4.0
94	8.0
95	13.0
96	14.0
97	7.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2101571911782747
2	0.007492914116218399
3	6.349927217134236E-4
4	0.0022097746715627146
5	0.0829046497469046
6	0.05455857464961737
7	0.02621249955233013
8	0.016205014258126572
9	0.014173037548643618
10-11	0.012128360984726392
12-13	0.01398253973212959
14-15	0.02186914933581031
16-17	0.024282121678321323
18-19	0.023342332450185455
20-21	0.017157503340696708
22-23	0.014579432890540207
24-25	0.012750653852005547
26-27	0.011937863168212366
28-29	0.014287336238552033
30-31	0.010845675686865277
32-33	0.01490962910583119
34-35	0.01764009780919891
36-37	0.018998982233665637
38-39	0.02264384045630069
40-41	0.023634429102173633
42-43	0.021513553411650795
44-45	0.01973557379085321
46-47	0.019811772917458823
48-49	0.018579887037334777
50-51	0.01497312837800253
52-53	0.012483956908885912
54-55	0.018262390676478068
56-57	0.021475453848347988
58-59	0.017538498973724764
60-61	0.019837172626327355
62-63	0.025450508286274025
64-65	0.01866878601837466
66-67	0.021475453848347988
68-69	0.015709719935190103
70-71	0.0163955120746406
72-73	0.024040824444070224
74-75	0.014592132744974479
76-77	0.01330944744711336
78-79	0.01614151498595523
80-81	0.01681460727097146
82-83	0.012725254143137013
84-85	0.01648441105568048
86-87	0.017221002612868054
88-89	0.01807189285996404
90-91	0.01314434933946787
92-93	0.01247125705445164
94-95	0.014007939440998127
96-97	0.014109538276472273
98-99	0.013182448902770675
100-101	0.017221002612868054
102-103	0.02331693274131692
104-105	0.007658012223863891
106-107	0.008127906837931824
108-109	0.014795330415922774
110-111	0.01164576651622419
112-113	0.00966458922447831
114-115	0.006438826198174116
116-117	0.00416555225444006
118-119	0.0022351743804312515
120-121	0.0024002724880767415
122-123	0.001600181658717828
124-125	5.07994177370739E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3937053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.2448040480114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.05448754018127	31.471664408835164
2	14.067500291638337	13.010975888643165
3	6.3906701499558	8.86605866460545
4	3.5702679285635965	6.6042536302129236
5	2.120163044246777	4.902326226551377
6	1.3758317226245034	3.817504104948489
7	0.9167706907121657	2.9677116664260916
8	0.6742919035465689	2.4945997560537343
9	0.5120676704919251	2.1312422173098677
>10	2.1981723293412827	16.916039565892312
>50	0.08485639828040015	2.6689412716738885
>100	0.032563078910454235	2.6866979242597617
>500	0.0016994139038977085	0.532174706780511
>1k	5.481980025535852E-4	0.5637117115402313
>5k	1.0963960051071612E-4	0.36609825626697445
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7912	0.20096249656786436	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6529	0.16583469920267774	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4508	0.11450188757936458	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT	3967	0.10076064508148608	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.523982532112217E-4
2	0.0	0.0	0.0	0.0	1.523982532112217E-4
3	0.0	0.0	0.0	0.0	1.523982532112217E-4
4	0.0	0.0	0.0	2.5399708868536947E-5	1.523982532112217E-4
5	0.0	0.0	0.0	2.5399708868536947E-5	1.523982532112217E-4
6	0.0	0.0	0.0	2.5399708868536947E-5	1.523982532112217E-4
7	0.0	0.0	0.0	2.5399708868536947E-5	1.523982532112217E-4
8	0.0	0.0	0.0	2.5399708868536947E-5	1.7779796207975865E-4
9	0.0	0.0	0.0	1.2699854434268474E-4	1.7779796207975865E-4
10-11	0.0	0.0	0.0	1.3969839877695322E-4	1.7779796207975865E-4
12-13	0.0	0.0	0.0	1.523982532112217E-4	4.0639534189659116E-4
14-15	0.0	0.0	0.0	1.7779796207975865E-4	4.82594468502202E-4
16-17	0.0	0.0	0.0	1.7779796207975865E-4	5.333938862392759E-4
18-19	0.0	0.0	0.0	1.904978165140271E-4	6.222928672791553E-4
20-21	0.0	0.0	0.0	2.41297234251101E-4	9.016896648330617E-4
22-23	0.0	0.0	0.0	3.682957785937857E-4	9.90588645872941E-4
24-25	0.0	0.0	0.0	0.001015988354741478	9.90588645872941E-4
26-27	0.0	0.0	0.0	0.0022351743804312515	0.0011302870446498942
28-29	0.0	0.0	0.0	0.005981631438540452	0.0011810864623869682
30-31	0.0	0.0	0.0	0.022808938563946182	0.0011937863168212366
32-33	0.0	0.0	0.0	0.06559474815299667	0.001231885880124042
34-35	0.0	0.0	0.0	0.13449145845890315	0.0013207848611639214
36-37	0.0	0.0	0.0	0.24736776467068133	0.0013207848611639214
38-39	0.0	0.0	0.0	0.42621981466848424	0.0013588844244667268
40-41	0.0	0.0	0.0	0.6226357633488805	0.0013715842789009953
42-43	0.0	0.0	0.0	0.8094887216402726	0.0013715842789009953
44-45	0.0	0.0	0.0	1.0345174423610757	0.0014096838422038007
46-47	0.0	0.0	0.0	1.310269381692347	0.0014477834055066062
48-49	0.0	0.0	0.0	1.602086636882968	0.0014477834055066062
50-51	0.0	0.0	0.0	1.896126366599586	0.0014731831143751431
52-53	0.0	0.0	0.0	2.1882103187333266	0.001523982532112217
54-55	0.0	0.0	0.0	2.513440890940508	0.001523982532112217
56-57	0.0	0.0	0.0	2.8874897035930176	0.001523982532112217
58-59	0.0	0.0	0.0	3.287763715652291	0.001523982532112217
60-61	0.0	0.0	0.0	3.7013471751586784	0.001523982532112217
62-63	0.0	0.0	0.0	4.110117389834477	0.0016001816587178277
64-65	0.0	0.0	0.0	4.549799050203287	0.0016255813675863646
66-67	0.0	0.0	0.0	5.044280582455964	0.0016255813675863646
68-69	0.0	0.0	0.0	5.587580355153969	0.0016255813675863646
70-71	0.0	0.0	0.0	6.178072786929716	0.0016255813675863646
72-73	0.0	0.0	0.0	6.754252482758043	0.0017525799119290494
74-75	0.0	0.0	0.0	7.33102907174478	0.0018922783107060027
76-77	0.0	0.0	0.0	7.966491688072271	0.001930377874008808
78-79	0.0	0.0	0.0	8.670635117180288	0.001930377874008808
80-81	0.0	0.0	0.0	9.405969388778866	0.001955777582877345
82-83	0.0	0.0	0.0	10.165280985549344	0.002031976709482956
84-85	0.0	0.0	0.0	10.91438189935467	0.002031976709482956
86-87	0.0	0.0	0.0	11.696299237018145	0.0021716751082599094
88-89	0.0	0.0	0.0	12.537994789503724	0.0023113735070368624
90-91	0.0	0.0	0.0	13.43098505404931	0.0023113735070368624
92-93	2.5399708868536947E-5	0.0	0.0	14.33775466065608	0.002438372051379547
94-95	2.5399708868536947E-5	0.0	0.0	15.243736876287924	0.002438372051379547
96-97	2.5399708868536947E-5	0.0	0.0	16.16075526542315	0.002590770304590769
98-99	2.5399708868536947E-5	0.0	0.0	17.139190150602495	0.0026923691400649167
100-101	2.5399708868536947E-5	0.0	0.0	18.188414024398455	0.0026923691400649167
102-103	2.5399708868536947E-5	0.0	0.0	19.241663752050073	0.0027304687033677224
104-105	2.5399708868536947E-5	0.0	0.0	20.276422491645402	0.0027685682666705276
106-107	2.5399708868536947E-5	0.0	0.0	21.311460628038283	0.0027685682666705276
108-109	2.5399708868536947E-5	0.0	0.0	22.390783156843455	0.0027685682666705276
110-111	2.5399708868536947E-5	0.0	0.0	23.5199399144487	0.0027685682666705276
112-113	2.5399708868536947E-5	0.0	0.0	24.67443288165031	0.0027939679755390645
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8655	0.0	42.117153	1
GTATCAA	13160	0.0	40.30238	1
TCAACGC	15835	0.0	33.05546	4
CAACGCA	16050	0.0	32.538456	5
ATCAACG	16280	0.0	32.151917	3
AACGCAG	16525	0.0	31.801987	6
TATCAAC	16790	0.0	31.317558	2
CATGGGG	8940	0.0	28.841827	4
ACGCAGA	18770	0.0	27.833027	7
TACATGG	15420	0.0	27.496252	2
GTACATG	15630	0.0	27.329954	1
CGCAGAG	19340	0.0	26.856153	8
ACATGGG	15450	0.0	26.478945	3
GAGTACT	11430	0.0	23.34723	12-13
GCAGAGT	21730	0.0	22.94552	9
AGAGTAC	19240	0.0	21.569992	10-11
AGTACTT	12515	0.0	21.394436	12-13
GTACTTT	12960	0.0	20.568003	14-15
CAGAGTA	20295	0.0	20.316788	10-11
ATGGGGG	5335	0.0	19.979794	5
>>END_MODULE