FastQCFastQC Report
Mon 15 Aug 2016
SRR2920928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4504868
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59250.13152438650810636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46180.1025113277458962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117200.037.6191331
GGTATCA82650.036.886721
TCAACGC139800.031.1386954
CAACGCA142350.030.4972175
ATCAACG146400.029.6942233
AACGCAG149050.029.1890456
TATCAAC150000.028.9818822
GTACATG176900.027.4191951
TACATGG183750.026.0569572
ACATGGG178950.025.5908573
CATGGGG123200.025.5702614
ACGCAGA171650.025.0973557
CGCAGAG179000.023.963648
GCAGAGT198450.021.1966769
GTATAGA12550.020.4416871
AGTACTT100950.020.27524212-13
GAGTACT99850.019.96230512-13
ATGGGGA66000.019.8503845
AGAGTAC180250.019.55811710-11
GTACTTT100700.019.46883214-15
Produced by FastQC (version 0.11.5)