##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920928_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4504868
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14375271373101	31.0	31.0	34.0	28.0	34.0
2	31.25492023295688	33.0	31.0	34.0	28.0	34.0
3	31.331831032562995	33.0	31.0	34.0	28.0	34.0
4	34.86938596203041	37.0	35.0	37.0	32.0	37.0
5	34.55391545323859	37.0	35.0	37.0	31.0	37.0
6	34.28969217300041	37.0	35.0	37.0	30.0	37.0
7	34.1968510509076	37.0	35.0	37.0	30.0	37.0
8	34.18491263229023	37.0	35.0	37.0	30.0	37.0
9	35.7481668719261	39.0	37.0	39.0	30.0	39.0
10-11	35.700121291012294	39.0	36.0	39.0	30.0	39.0
12-13	35.589802298313735	39.0	35.0	39.0	30.0	39.0
14-15	36.83068904571677	40.0	37.0	41.0	30.0	41.0
16-17	36.82660757207536	40.0	37.0	41.0	30.0	41.0
18-19	36.85251898612789	40.0	37.0	41.0	30.0	41.0
20-21	36.83901026622756	40.0	37.0	41.0	30.0	41.0
22-23	36.7902766518353	40.0	37.0	41.0	30.0	41.0
24-25	36.76736954334733	40.0	37.0	41.0	30.0	41.0
26-27	36.644394907908506	40.0	37.0	41.0	30.0	41.0
28-29	36.55384208815885	40.0	36.5	41.0	30.0	41.0
30-31	36.42080622562082	40.0	36.0	41.0	29.0	41.0
32-33	36.29026166804444	40.0	36.0	41.0	29.0	41.0
34-35	36.186150404406966	40.0	36.0	41.0	28.5	41.0
36-37	36.066055209608805	40.0	35.5	41.0	28.0	41.0
38-39	35.95455316337792	40.0	35.0	41.0	27.0	41.0
40-41	35.81649628801554	40.0	35.0	41.0	27.0	41.0
42-43	35.67893043702945	40.0	35.0	41.0	26.5	41.0
44-45	35.495500867062034	40.0	35.0	41.0	26.0	41.0
46-47	35.30773310116967	39.0	35.0	41.0	25.5	41.0
48-49	35.06898248738919	39.0	35.0	41.0	24.0	41.0
50-51	34.874012956650446	39.0	34.5	41.0	24.0	41.0
52-53	34.663013100494844	39.0	34.0	41.0	23.0	41.0
54-55	34.43801793970434	38.5	34.0	41.0	23.0	41.0
56-57	34.16694939785139	38.0	34.0	40.5	22.0	41.0
58-59	33.91297658444154	37.5	33.5	40.0	22.0	41.0
60-61	33.62484183776306	37.0	33.0	40.0	21.5	41.0
62-63	33.3093661123922	36.5	33.0	40.0	20.5	41.0
64-65	32.97682984273901	36.0	33.0	39.0	20.0	41.0
66-67	32.637257406876294	35.5	32.5	39.0	18.5	41.0
68-69	32.261133622561196	35.0	32.0	38.5	18.0	40.5
70-71	31.917812020241215	35.0	32.0	37.5	17.5	40.0
72-73	31.559202511594123	35.0	31.5	37.0	14.0	39.0
74-75	31.239062276630523	35.0	31.0	36.5	12.5	39.0
76-77	31.039808047649785	35.0	31.0	36.0	12.0	39.0
78-79	30.90694311131869	35.0	31.0	36.0	11.0	37.5
80-81	30.677428506229262	35.0	31.0	35.0	9.0	37.0
82-83	30.484538059716733	35.0	31.0	35.0	7.0	37.0
84-85	30.273395469079226	35.0	31.0	35.0	7.0	36.0
86-87	30.13159875050723	35.0	31.0	35.0	6.5	36.0
88-89	29.999052469461922	35.0	31.0	35.0	2.0	36.0
90-91	29.85640722436262	35.0	31.0	35.0	2.0	35.0
92-93	29.749206192057123	35.0	31.0	35.0	2.0	35.0
94-95	29.67420665821951	35.0	31.0	35.0	2.0	35.0
96-97	29.60985016653096	35.0	31.0	35.0	2.0	35.0
98-99	29.511828759466425	34.0	30.5	35.0	2.0	35.0
100-101	29.41744186511125	34.0	30.0	35.0	2.0	35.0
102-103	27.98305621829541	32.5	28.0	34.0	2.0	35.0
104-105	29.075745171667627	34.0	29.5	35.0	2.0	35.0
106-107	29.265701347964026	34.0	30.0	35.0	2.0	35.0
108-109	29.37511520870312	34.5	30.5	35.0	2.0	35.0
110-111	29.56313736606711	35.0	31.0	35.0	2.0	35.0
112-113	29.551051662335055	35.0	31.0	35.0	2.0	35.0
114-115	29.4753766370069	35.0	31.0	35.0	2.0	35.0
116-117	29.372371399117576	35.0	31.0	35.0	2.0	35.0
118-119	29.282254663177703	35.0	31.0	35.0	2.0	35.0
120-121	29.113518864481712	35.0	31.0	35.0	2.0	35.0
122-123	28.94043654553252	35.0	31.0	35.0	2.0	35.0
124-125	28.20014337822995	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89077.0
3	33881.0
4	15040.0
5	11872.0
6	13287.0
7	15759.0
8	17460.0
9	19545.0
10	21648.0
11	23555.0
12	24619.0
13	27181.0
14	31188.0
15	28179.0
16	19538.0
17	17745.0
18	16806.0
19	16163.0
20	16175.0
21	16469.0
22	17717.0
23	21206.0
24	28375.0
25	37900.0
26	47120.0
27	56651.0
28	67541.0
29	82025.0
30	98351.0
31	119811.0
32	149555.0
33	191242.0
34	259127.0
35	391652.0
36	764168.0
37	1285998.0
38	410379.0
39	863.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.345048150749186	17.52419883519922	11.69570141307895	26.43505160097265
2	16.985027947446635	19.861201822344903	36.56548990948215	26.58828032072631
3	19.674070772277748	23.273330502306074	28.826842578087625	28.22575614732855
4	13.727181651953721	15.364093516129484	34.55797785588276	36.35074697603403
5	14.41499504461843	36.81025387416197	33.90791765779852	14.866833423421083
6	34.933227969272075	34.67694721402675	15.978779713598094	14.411045103103087
7	29.590624342895993	31.145580830537277	20.464519658775533	18.799275167791198
8	27.711561581062945	33.976690566661894	19.210378792167727	19.101369060107434
9	27.014744946722598	14.42156361040838	18.476485012504842	40.08720643036418
10-11	26.35804691269797	24.96006356528379	26.900334354902395	21.781555167115844
12-13	26.630717003299765	22.192963507221272	27.233013362236335	23.94330612724263
14-15	23.922250645518936	23.47428675972086	24.350997999637872	28.252464595122333
16-17	24.09873736804634	26.467744562294136	25.170319817247922	24.2631982524116
18-19	24.25202782334379	25.487394767669574	26.640376531691594	23.62020087729504
20-21	24.87458464541515	24.835764748091947	26.45083677026891	23.83881383622399
22-23	24.533618487795515	24.56957361482013	26.357228340366174	24.539579557018182
24-25	24.2625641494706	24.967020578448622	26.49254360920751	24.277871662873274
26-27	24.088883018017594	25.047801041105288	26.48800963957599	24.37530630130113
28-29	24.14255694918227	25.12476591474468	26.548759112257684	24.183918023815366
30-31	24.243169303675575	24.99247959055228	26.39707007067964	24.367281035092507
32-33	24.07634210950187	25.12581244562743	26.302485588806686	24.495359856064013
34-35	24.299563098017707	25.090187283828875	26.48266318272942	24.127586435423996
36-37	24.393263417498247	24.93135876290468	26.27751852256016	24.397859297036913
38-39	24.276500184229693	25.039130076155303	26.279269568951364	24.405100170663637
40-41	24.38290272186238	24.990741209477804	26.345999447581036	24.28035662107878
42-43	24.39968551916093	24.98455238324033	26.22396968337215	24.391792414226597
44-45	24.28895139605379	25.03307602968639	26.249301456899477	24.42867111736035
46-47	24.37568341953439	25.141413031291037	26.13863661341406	24.344266935760505
48-49	24.348241460574112	25.312670366954116	25.822232341477346	24.516855830994427
50-51	24.28105802043993	25.441990771771287	25.857589644207224	24.419361563581553
52-53	24.47912654305022	25.26057825961762	25.776051332179918	24.484243865152237
54-55	24.24798049409512	25.325583161192807	25.767572099202678	24.658864245509395
56-57	24.233189858252306	25.465727839627633	25.708605263239694	24.59247703888037
58-59	24.098911082465644	25.13095828561761	25.909669976683496	24.860460655233247
60-61	24.069595347077534	25.30109848827977	26.06724943650189	24.562056728140803
62-63	23.91773524460963	25.35678783372083	26.07709126241444	24.6483856592551
64-65	23.80011325336327	25.357807693746565	25.955368494717156	24.886710558173007
66-67	23.837949872499813	25.451934594680402	25.948302754381462	24.761812778438312
68-69	24.161945126526916	25.09195902472814	25.96877789075925	24.77731795798569
70-71	23.81938137765509	25.16808097237361	26.001878044342853	25.010659605628447
72-73	23.927178124572933	25.19457130921221	25.919079872681174	24.95917069353369
74-75	23.799963501040576	25.31290531272992	25.86641736071673	25.020713825512775
76-77	23.859077973490482	25.04908358256704	25.975252794187075	25.116585649755407
78-79	23.780196475797528	25.222937954356944	25.910156992367956	25.086708577477573
80-81	23.785276284059904	25.299960047880376	25.8469899313776	25.06777373668212
82-83	23.592546538070206	25.186481964104264	25.96147079150407	25.259500706321457
84-85	23.457196058837255	25.309597609139832	25.82234662099622	25.41085971102669
86-87	23.502847223694637	25.09809620049357	25.96688879270544	25.43216778310635
88-89	23.43948136702537	25.11611993726121	25.79465046236499	25.64974823334843
90-91	23.29057941027071	25.224318784877752	25.879370042519334	25.605731762332205
92-93	23.329095913183654	25.084025022239796	26.0307953732345	25.556083691342053
94-95	23.183983098692863	24.934412191817227	26.107300033501566	25.774304675988347
96-97	23.24988438648018	24.710844093031643	26.10331264093693	25.935958879551247
98-99	23.173594965247037	24.945227247120386	25.912193757399194	25.968984030233383
100-101	23.054332598448973	24.759186609563265	26.06216322425518	26.12431756773258
102-103	22.879596897465767	24.617414836785237	26.14303732137709	26.359950944371906
104-105	22.74758759890306	24.912260682951434	25.943754041758343	26.396397676387164
106-107	22.695088441573503	24.793088731282026	25.964709950155708	26.547112876988766
108-109	22.61654441483522	24.762728432647823	26.099694797797714	26.521032354719242
110-111	22.58543050121455	24.84145043395288	26.02790392439661	26.545215140435957
112-113	22.5215845102355	24.929461351014236	25.996829132464804	26.55212500628546
114-115	22.389344795450317	25.132942530909663	25.818267549146668	26.65944512449335
116-117	22.218253521189293	25.006057572187224	25.981840047905603	26.793848858717883
118-119	22.269009742467	24.988686972953627	25.841251342329368	26.90105194225001
120-121	22.11310305390366	25.05781108378939	25.894426316701956	26.934659545604994
122-123	21.959984425487857	25.12077128464863	25.82094727485995	27.098297015003563
124-125	21.885992684538916	25.22609835970958	25.801867656860367	27.08604129889114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	79.0
1	94.5
2	215.0
3	362.5
4	287.0
5	191.5
6	327.5
7	667.0
8	1069.0
9	1215.0
10	1076.5
11	943.5
12	1004.0
13	1174.0
14	1353.5
15	1502.0
16	1654.5
17	1846.5
18	2159.5
19	2463.0
20	2819.5
21	3293.0
22	3905.0
23	4825.0
24	5873.0
25	7502.5
26	9941.0
27	12889.0
28	16016.5
29	20231.5
30	24923.5
31	30489.0
32	39113.0
33	43775.0
34	45971.0
35	51154.5
36	58460.5
37	66853.0
38	76239.5
39	85129.5
40	93748.5
41	103360.0
42	111099.0
43	122522.5
44	135512.5
45	146662.5
46	159092.0
47	169308.5
48	179165.5
49	191571.5
50	203327.0
51	209912.0
52	212810.5
53	216648.5
54	214979.5
55	206677.0
56	194422.0
57	176273.0
58	158950.0
59	142643.5
60	122922.5
61	101794.0
62	81192.0
63	60815.5
64	43156.0
65	31204.5
66	22738.0
67	16730.5
68	11965.5
69	8540.0
70	6687.0
71	4960.0
72	3719.0
73	3083.5
74	2382.0
75	1843.0
76	1330.5
77	790.0
78	476.5
79	277.0
80	161.0
81	100.5
82	60.0
83	41.5
84	29.0
85	22.5
86	14.0
87	9.0
88	8.0
89	6.0
90	7.5
91	8.0
92	5.0
93	4.5
94	7.0
95	12.0
96	11.0
97	8.0
98	7.0
99	3.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21103837004769066
2	0.0075917873731261385
3	5.54955217333782E-4
4	0.0023308119128018843
5	0.0821555703740931
6	0.05292052952494946
7	0.02674884147548829
8	0.015161376537558925
9	0.013607501929024336
10-11	0.011598564042276044
12-13	0.013474312676864228
14-15	0.020722027815243422
16-17	0.023563398527992385
18-19	0.022730965701991714
20-21	0.016304584285266516
22-23	0.014262349085478198
24-25	0.012075825529183097
26-27	0.011554167624889342
28-29	0.01405146610289136
30-31	0.010188977790248238
32-33	0.01428454729417155
34-35	0.01705932338084046
36-37	0.018502206945908293
38-39	0.02174314541513758
40-41	0.023086137041085333
42-43	0.02079972154567015
44-45	0.01889067559804194
46-47	0.019157054102362157
48-49	0.017836260685107755
50-51	0.014317844607211576
52-53	0.012408798659583367
54-55	0.01766977411990762
56-57	0.021088298258683715
58-59	0.016915035024333676
60-61	0.0190904594762821
62-63	0.02532815611911381
64-65	0.01792505351988116
66-67	0.02115489288476377
68-69	0.015272367581025681
70-71	0.015860620111399493
72-73	0.023452407484525626
74-75	0.013984871476811309
76-77	0.012464294181316744
78-79	0.015538746085345896
80-81	0.016126998615719708
82-83	0.012098023737876449
84-85	0.015871719215746167
86-87	0.01650436816350668
88-89	0.017680873224254295
90-91	0.012519789703050122
92-93	0.01232000582480996
94-95	0.013341123424704119
96-97	0.014140258937664766
98-99	0.012775069103023663
100-101	0.016826242189560272
102-103	0.022231506006391306
104-105	0.007347607077499274
106-107	0.007558490060086112
108-109	0.014229051772438172
110-111	0.01153196941619599
112-113	0.009378743172940917
114-115	0.006437480521071872
116-117	0.00389578562568315
118-119	0.0023419110171485603
120-121	0.0024196047475752895
122-123	0.001598271025921292
124-125	5.660543216804576E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4504868.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.75316671392042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.80674671594848	26.95532906527676
2	13.799417907511954	10.971411212647633
3	6.104510651328223	7.280208888874613
4	3.392926018766293	5.39518214688059
5	2.059437422148963	4.093457958978712
6	1.3417084719168775	3.2002296339354626
7	0.9962862409352106	2.7723873121477762
8	0.7106789455205583	2.2601390881081542
9	0.5264457020454419	1.8835095383315397
>10	2.959354098247406	21.41332046883243
>50	0.2072821447882365	5.656144360447978
>100	0.09034653753810996	6.063501668171007
>500	0.003295281276260538	0.9170294309691228
>1k	0.0015080098127330287	1.0065963076800906
>5k	5.585221528639878E-5	0.13155291871810265
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5925	0.13152438650810636	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4618	0.1025113277458962	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2198208693351283E-5
2	0.0	0.0	0.0	0.0	2.2198208693351283E-5
3	0.0	0.0	0.0	0.0	2.2198208693351283E-5
4	0.0	0.0	0.0	0.0	2.2198208693351283E-5
5	0.0	0.0	0.0	0.0	4.4396417386702566E-5
6	0.0	0.0	0.0	0.0	4.4396417386702566E-5
7	0.0	0.0	0.0	0.0	4.4396417386702566E-5
8	0.0	0.0	0.0	0.0	4.4396417386702566E-5
9	0.0	0.0	0.0	6.659462608005384E-5	4.4396417386702566E-5
10-11	0.0	0.0	0.0	6.659462608005384E-5	6.659462608005384E-5
12-13	0.0	0.0	0.0	6.659462608005384E-5	2.3308119128018847E-4
14-15	0.0	0.0	0.0	6.659462608005384E-5	3.107749217069179E-4
16-17	0.0	0.0	0.0	6.659462608005384E-5	3.773695477869718E-4
18-19	0.0	0.0	0.0	6.659462608005384E-5	4.883605912537282E-4
20-21	0.0	0.0	0.0	8.879283477340513E-5	5.993516347204846E-4
22-23	0.0	0.0	0.0	1.2209014781343206E-4	6.215498434138358E-4
24-25	0.0	0.0	0.0	1.8868477389348591E-4	6.437480521071871E-4
26-27	0.0	0.0	0.0	4.3286506952035003E-4	6.659462608005384E-4
28-29	0.0	0.0	0.0	0.0021865235562951012	7.10342678187241E-4
30-31	0.0	0.0	0.0	0.008968076312113918	7.325408868805922E-4
32-33	0.0	0.0	0.0	0.025083975823486947	7.769373042672948E-4
34-35	0.0	0.0	0.0	0.05284283579452272	8.213337216539975E-4
36-37	0.0	0.0	0.0	0.10072437194608144	8.213337216539975E-4
38-39	0.0	2.2198208693351283E-5	0.0	0.17935042713793167	9.101265564274026E-4
40-41	0.0	2.2198208693351283E-5	0.0	0.2690422893634175	9.323247651207539E-4
42-43	0.0	2.2198208693351283E-5	0.0	0.3579128178672494	9.545229738141051E-4
44-45	0.0	2.2198208693351283E-5	0.0	0.46849319447317883	9.656220781607808E-4
46-47	0.0	2.2198208693351283E-5	0.0	0.6044905200329954	0.0010100184955474834
48-49	0.0	2.2198208693351283E-5	0.0	0.7487788765397787	0.0010877122259742129
50-51	0.0	2.2198208693351283E-5	0.0	0.904943274697505	0.0011321086433609153
52-53	0.0	2.2198208693351283E-5	0.0	1.061285258524778	0.001143207747707591
54-55	0.0	2.2198208693351283E-5	0.0	1.2347975567763583	0.0011765050607476179
56-57	0.0	2.2198208693351283E-5	0.0	1.445258773398022	0.0012209014781343205
58-59	0.0	2.2198208693351283E-5	0.0	1.6787950279564239	0.001265297895521023
60-61	0.0	2.2198208693351283E-5	0.0	1.9263383522003308	0.001265297895521023
62-63	0.0	2.2198208693351283E-5	0.0	2.181340274565204	0.0013762889389877795
64-65	0.0	2.2198208693351283E-5	0.0	2.453667898815237	0.0013984871476811307
66-67	0.0	2.2198208693351283E-5	0.0	2.7779171331990193	0.001420685356374482
68-69	0.0	2.2198208693351283E-5	0.0	3.1426780984481675	0.0014428835650678333
70-71	0.0	2.2198208693351283E-5	0.0	3.537351149911607	0.0014761808781078602
72-73	0.0	2.2198208693351283E-5	0.0	3.9364527440093697	0.0015760728172279411
74-75	0.0	2.2198208693351283E-5	0.0	4.343889765471486	0.0016537665476546704
76-77	0.0	2.2198208693351283E-5	0.0	4.803104108710843	0.0016870638606946973
78-79	0.0	2.2198208693351283E-5	0.0	5.32198723691793	0.0017203611737347242
80-81	0.0	2.2198208693351283E-5	0.0	5.874633840547603	0.001753658486774751
82-83	0.0	2.2198208693351283E-5	0.0	6.445338686949318	0.0018202531128548051
84-85	0.0	2.2198208693351283E-5	0.0	7.01331315368175	0.0018646495302415077
86-87	0.0	2.2198208693351283E-5	0.0	7.618036754906026	0.001975640573708264
88-89	0.0	2.2198208693351283E-5	0.0	8.282584528558882	0.0021199289302150474
90-91	0.0	2.2198208693351283E-5	0.0	9.004670059144907	0.002131028034561723
92-93	0.0	2.2198208693351283E-5	0.0	9.738232063625393	0.002219820869335128
94-95	0.0	2.2198208693351283E-5	0.0	10.4622155410547	0.002286415495415182
96-97	0.0	2.2198208693351283E-5	0.0	11.215578347689654	0.0024307038519219655
98-99	0.0	2.2198208693351283E-5	0.0	12.018032492849958	0.002552793999735397
100-101	0.0	2.2198208693351283E-5	0.0	12.891709590602876	0.002552793999735397
102-103	0.0	2.2198208693351283E-5	0.0	13.785553760953706	0.002586091312775424
104-105	0.0	2.2198208693351283E-5	0.0	14.662338607923694	0.0026193886258154514
106-107	0.0	2.2198208693351283E-5	0.0	15.536626156415682	0.0026193886258154514
108-109	0.0	2.2198208693351283E-5	0.0	16.454333401111864	0.0026193886258154514
110-111	0.0	2.2198208693351283E-5	0.0	17.433352098219082	0.002708181460588856
112-113	0.0	2.2198208693351283E-5	0.0	18.45112886770489	0.0027303796692822076
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11720	0.0	37.619133	1
GGTATCA	8265	0.0	36.88672	1
TCAACGC	13980	0.0	31.138695	4
CAACGCA	14235	0.0	30.497217	5
ATCAACG	14640	0.0	29.694223	3
AACGCAG	14905	0.0	29.189045	6
TATCAAC	15000	0.0	28.981882	2
GTACATG	17690	0.0	27.419195	1
TACATGG	18375	0.0	26.056957	2
ACATGGG	17895	0.0	25.590857	3
CATGGGG	12320	0.0	25.570261	4
ACGCAGA	17165	0.0	25.097355	7
CGCAGAG	17900	0.0	23.96364	8
GCAGAGT	19845	0.0	21.196676	9
GTATAGA	1255	0.0	20.441687	1
AGTACTT	10095	0.0	20.275242	12-13
GAGTACT	9985	0.0	19.962305	12-13
ATGGGGA	6600	0.0	19.850384	5
AGAGTAC	18025	0.0	19.558117	10-11
GTACTTT	10070	0.0	19.468832	14-15
>>END_MODULE