FastQCFastQC Report
Mon 15 Aug 2016
SRR2920927_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920927_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404159
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA2400.032.237257
GTATCAA58500.031.5766051
GTACATG96650.029.2854141
ACCGTAT3100.028.7951478
TACATGG98000.027.9588572
CATGGGG64800.027.9432684
TCAACGC64500.027.242124
GGTATCA43650.026.7566321
AACGCAG66750.026.6624496
TATCAAC68750.026.3383482
ACATGGG102100.026.0777073
CAACGCA67350.026.0008985
ATCAACG68900.025.6758543
ACGCAGA75100.023.6157767
CGCAGAG78650.022.2453048
TATACCG3108.352799E-921.1353575
GTATAGA6800.020.1548021
CGTACAC4152.910383E-1120.0940553
GCAGAGT84750.019.9419849
CCGTATG3652.586603E-919.5649229
Produced by FastQC (version 0.11.5)