FastQCFastQC Report
Mon 15 Aug 2016
SRR2920927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404159
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56830.23638203629626825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44830.18646853223934023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.14154637858810504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51550.046.871051
GTATCAA78850.041.462661
TCAACGC94900.033.8202864
CAACGCA96800.033.0949445
ATCAACG99300.032.32173
TATCAAC100500.032.1727752
AACGCAG100500.031.9178336
GTACATG98150.030.3919141
TACATGG99650.029.8179652
CATGGGG67200.028.8870414
ACATGGG103650.028.092753
ACGCAGA114800.027.8336897
CGCAGAG120600.026.4413498
GTATAGA7450.025.6254831
GCAGAGT132450.023.763759
GAGTACT73850.023.44665312-13
AGTACTT77050.022.51149212-13
CAGAGTA126000.021.77051710-11
AGAGTAC119600.021.59220110-11
TACCGTA2505.280672E-721.4210557
Produced by FastQC (version 0.11.5)