##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920927_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2404159 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.182891397781926 31.0 31.0 34.0 28.0 34.0 2 31.273948187287115 33.0 31.0 34.0 28.0 34.0 3 31.367602974678462 33.0 31.0 34.0 28.0 34.0 4 34.92024321186744 37.0 35.0 37.0 32.0 37.0 5 34.644330096303946 37.0 35.0 37.0 32.0 37.0 6 34.363388195206724 37.0 35.0 37.0 30.0 37.0 7 34.25560705427553 37.0 35.0 37.0 30.0 37.0 8 34.253419179014365 37.0 35.0 37.0 30.0 37.0 9 35.8088599797268 39.0 37.0 39.0 30.0 39.0 10-11 35.78602496756662 39.0 36.0 39.0 30.0 39.0 12-13 35.694869182945055 39.0 35.0 39.0 30.0 39.0 14-15 36.92156633567081 40.0 37.0 41.0 30.0 41.0 16-17 36.93120130573727 40.0 37.0 41.0 30.0 41.0 18-19 36.968329049784145 40.0 37.0 41.0 30.0 41.0 20-21 36.96289471702995 40.0 37.0 41.0 30.0 41.0 22-23 36.92028563834588 40.0 37.0 41.0 30.0 41.0 24-25 36.90835610290334 40.0 37.0 41.0 30.0 41.0 26-27 36.78718899207581 40.0 37.0 41.0 30.0 41.0 28-29 36.68549854647716 40.0 36.5 41.0 30.0 41.0 30-31 36.53469550058877 40.0 36.0 41.0 30.0 41.0 32-33 36.37806879661453 40.0 36.0 41.0 29.5 41.0 34-35 36.26359404681637 40.0 36.0 41.0 29.0 41.0 36-37 36.13501935604093 40.0 35.0 41.0 28.0 41.0 38-39 36.01189937936717 40.0 35.0 41.0 28.0 41.0 40-41 35.864818217097955 40.0 35.0 41.0 27.0 41.0 42-43 35.72010441073157 40.0 35.0 41.0 27.0 41.0 44-45 35.5203669973575 40.0 35.0 41.0 26.0 41.0 46-47 35.28889395418523 39.5 35.0 41.0 24.5 41.0 48-49 35.02680812708311 39.0 35.0 41.0 24.0 41.0 50-51 34.834388449349646 39.0 35.0 41.0 23.5 41.0 52-53 34.63181802867447 39.0 34.0 41.0 23.0 41.0 54-55 34.424303883395396 39.0 34.0 41.0 22.5 41.0 56-57 34.17024705936671 38.0 34.0 41.0 22.0 41.0 58-59 33.93228297296477 38.0 34.0 40.0 21.5 41.0 60-61 33.65842982930829 37.0 33.0 40.0 20.0 41.0 62-63 33.34996583004701 37.0 33.0 40.0 19.5 41.0 64-65 33.038114159670805 36.0 33.0 39.5 18.0 41.0 66-67 32.71100039556452 36.0 32.5 39.0 17.5 41.0 68-69 32.33436619624575 35.0 32.0 39.0 14.5 41.0 70-71 31.994341472423415 35.0 32.0 38.5 13.0 40.0 72-73 31.632095880513724 35.0 32.0 37.0 10.5 40.0 74-75 31.30740624892114 35.0 31.0 37.0 8.0 39.0 76-77 31.09660550737285 35.0 31.5 36.0 7.5 39.0 78-79 30.949541606857117 35.0 32.0 36.0 7.0 38.5 80-81 30.705630742392664 35.0 31.0 36.0 6.5 37.0 82-83 30.490023122430756 35.0 31.0 35.0 3.5 37.0 84-85 30.25528303244503 35.0 31.0 35.0 2.0 36.5 86-87 30.09572536591798 35.0 31.0 35.0 2.0 36.0 88-89 29.94570138663874 35.0 31.0 35.0 2.0 36.0 90-91 29.793740763402088 35.0 31.0 35.0 2.0 36.0 92-93 29.67462301786196 35.0 31.0 35.0 2.0 35.0 94-95 29.583072292639546 35.0 31.0 35.0 2.0 35.0 96-97 29.515062855659714 35.0 31.0 35.0 2.0 35.0 98-99 29.414095739924022 35.0 30.5 35.0 2.0 35.0 100-101 29.3163133137201 34.0 30.0 35.0 2.0 35.0 102-103 27.902924681770216 32.5 27.5 34.5 2.0 35.0 104-105 28.971659944288213 34.0 29.5 35.0 2.0 35.0 106-107 29.14847582876174 34.0 30.0 35.0 2.0 35.0 108-109 29.240594320092804 34.5 30.5 35.0 2.0 35.0 110-111 29.418501646521715 35.0 31.0 35.0 2.0 35.0 112-113 29.390394520495526 35.0 31.0 35.0 2.0 35.0 114-115 29.29890618715318 35.0 31.0 35.0 2.0 35.0 116-117 29.1800619260207 35.0 31.0 35.0 2.0 35.0 118-119 29.072646609479655 35.0 31.0 35.0 2.0 35.0 120-121 28.88431900718713 35.0 31.0 35.0 2.0 35.0 122-123 28.692499123394086 35.0 31.0 35.0 2.0 35.0 124-125 27.947235810942622 34.5 28.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43919.0 3 17376.0 4 6949.0 5 5932.0 6 6866.0 7 8205.0 8 9234.0 9 10241.0 10 11749.0 11 13425.0 12 14472.0 13 16476.0 14 20569.0 15 18533.0 16 11794.0 17 10096.0 18 9803.0 19 9508.0 20 9286.0 21 9638.0 22 9894.0 23 11129.0 24 15002.0 25 19714.0 26 24991.0 27 29540.0 28 35575.0 29 42929.0 30 51826.0 31 63056.0 32 78840.0 33 100101.0 34 135209.0 35 201925.0 36 381651.0 37 672028.0 38 265623.0 39 1055.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.524041958537495 18.77688320512206 12.34308794994654 25.355986886393904 2 16.74256255639154 20.7723867915777 37.271849551009986 25.213201101020775 3 19.117399324915926 24.370659839568745 29.790299669945032 26.721641165570297 4 13.444804200830582 16.168322729208818 35.59213109626238 34.79474197369822 5 13.726614188700045 37.52008096077224 34.295679164215734 14.457625686311976 6 33.09163142338467 36.026139264581396 16.487030666441377 14.395198645592558 7 28.184098956101334 32.18869227664771 21.18510998589551 18.442098781355444 8 26.756498152893798 34.70587745864812 19.86658401837122 18.671040370086864 9 26.7608540010342 14.636547020934849 19.1512490062572 39.45134997177375 10-11 25.65962141422831 25.532657942018627 27.646504074104733 21.161216569648325 12-13 25.98416876664687 23.015089696802033 27.96782584187279 23.032915694678305 14-15 23.489669022210453 24.272841740199837 25.21745841325617 27.020030824333542 16-17 23.157632186710416 27.25163726502995 26.123597959851903 23.467132588407736 18-19 23.220550567727592 26.022129133974893 28.16321198415609 22.594108314141423 20-21 23.9093431503249 25.316098128590912 27.97256007190511 22.801998649179076 22-23 23.583790477058155 25.165983057133452 27.822107181404988 23.428119284403408 24-25 23.259174394459496 25.409477858919345 27.958521081102667 23.372826665518495 26-27 23.128881404597085 25.44423018163159 28.06165693817354 23.365231475597785 28-29 23.22806646782702 25.5474139786948 28.088058825695118 23.13646072778306 30-31 23.35478626729349 25.443233756276868 27.91021716259677 23.29176281383288 32-33 23.16358338627099 25.610821496723275 27.833953400719118 23.391641716286617 34-35 23.327815478726237 25.608558762447416 27.922833517492947 23.140792241333404 36-37 23.50973981264647 25.469995935417657 27.799704704140428 23.220559547795453 38-39 23.407343336686644 25.527813765552754 27.717451449787276 23.347391447973326 40-41 23.43292508516005 25.511131913565798 27.754655849390232 23.301287151883923 42-43 23.350934569913118 25.41831712929545 27.819300812649594 23.41144748814184 44-45 23.305943869384272 25.589584889552874 27.58716233546196 23.517308905600892 46-47 23.38018424456442 25.65008343550421 27.386606968643147 23.583125351288224 48-49 23.396669050492594 25.813319388159666 27.136405182179637 23.653606379168107 50-51 23.358871736864515 25.983170987352967 27.037683168345666 23.620274107436853 52-53 23.38134015579337 25.970255738250497 27.017128950734765 23.631275155221367 54-55 23.265059902628977 25.987725135390654 26.94801851734363 23.799196444636742 56-57 23.270442606517232 26.037027167637394 26.797652784027047 23.89487744181833 58-59 23.11095219017594 25.88262482469814 27.016413370825358 23.990009614300554 60-61 23.234503837182718 26.039902207121123 27.003207411723373 23.722386543972785 62-63 23.010326924269084 25.960787560593346 27.0808933386533 23.94799217648427 64-65 22.95754599532262 25.91470092613644 27.00337875575338 24.12437432278756 66-67 22.967597045774518 26.081831979257846 26.870895753384076 24.079675221583553 68-69 23.10622661858906 25.787139066938046 26.821168152711227 24.28546616176166 70-71 22.905654286206378 25.729386030234625 26.982602900320916 24.38235678323808 72-73 22.93869715620891 25.748829484244972 26.855096109500405 24.457377250045713 74-75 22.917372588296107 25.897500699421432 26.66806030936788 24.517066402914587 76-77 22.796076551998414 25.650198601882686 26.869037575442924 24.68468727067598 78-79 22.833538151511092 25.846424640084404 26.756499614353856 24.56353759405065 80-81 22.83940088437075 25.837121522558775 26.629656331812512 24.693821261257966 82-83 22.66638017875265 25.58171364007647 26.77560279462997 24.976303386540906 84-85 22.609543790370843 25.6956218677593 26.625031383509924 25.069802958359933 86-87 22.633994299628533 25.600895784545763 26.66789532650248 25.097214589323226 88-89 22.478014020936968 25.446386411564546 26.689017015914274 25.38658255158421 90-91 22.448481238089336 25.54060535819252 26.699691700712986 25.31122170300516 92-93 22.43809650590669 25.47446432118577 26.706658194120454 25.380780978787087 94-95 22.313090357159126 25.21500955678921 26.87320452693737 25.598695559114287 96-97 22.295823555375655 25.076743360799668 26.80131092927796 25.826122154546717 98-99 22.229206516665652 25.26225078063704 26.649425952011104 25.8591167506862 100-101 22.168435311208775 25.156860771036943 26.763659222107027 25.911044695647256 102-103 22.02172373228823 25.059073033386483 26.735438723494102 26.183764510831182 104-105 21.89075856595217 25.258777167000733 26.583679655633507 26.266784611413584 106-107 21.854705880273027 25.18533601753285 26.656813416449506 26.303144685744616 108-109 21.77024560454704 25.21428385707823 26.72462441692971 26.29084612144503 110-111 21.7044561806442 25.284879944306322 26.608582392469454 26.40208148258002 112-113 21.651572195445308 25.393987434250498 26.5675519792605 26.386888391043694 114-115 21.477382912902282 25.566420313469084 26.475529752275477 26.480667021353156 116-117 21.322308893004625 25.480870322091555 26.54380467980629 26.65301610509754 118-119 21.353010004995635 25.49252339441928 26.503089099076206 26.651377501508883 120-121 21.316924431019242 25.590288097237156 26.50601482508122 26.586772646662382 122-123 21.225372465078667 25.664588842976926 26.281946227265053 26.828092464679354 124-125 20.997243720380602 25.731647385049122 26.336165454143494 26.93494344042678 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 116.0 1 116.0 2 250.0 3 446.5 4 337.0 5 224.0 6 402.0 7 895.0 8 1438.5 9 1533.5 10 1253.5 11 990.0 12 1007.5 13 1151.0 14 1297.0 15 1441.0 16 1618.5 17 1837.5 18 2070.0 19 2322.5 20 2701.0 21 3145.0 22 3630.0 23 4411.5 24 5438.5 25 6741.0 26 8345.5 27 10160.5 28 12322.5 29 15189.0 30 17885.5 31 20894.0 32 24935.5 33 28384.5 34 32091.5 35 35817.0 36 40331.5 37 45160.0 38 50082.5 39 55035.0 40 59531.5 41 64381.5 42 68986.0 43 75281.0 44 80701.5 45 84925.0 46 90315.5 47 94751.5 48 99267.5 49 103829.0 50 107456.0 51 107593.0 52 104271.0 53 103274.5 54 101322.0 55 95593.0 56 86428.5 57 77080.0 58 70005.5 59 61970.5 60 52194.5 61 42907.5 62 34902.5 63 26153.5 64 18128.5 65 12717.5 66 9397.0 67 7099.5 68 5303.0 69 4009.0 70 3057.5 71 2198.5 72 1574.5 73 1258.5 74 983.0 75 683.0 76 468.0 77 268.0 78 126.5 79 81.0 80 62.0 81 60.0 82 44.0 83 28.5 84 20.5 85 8.5 86 8.5 87 9.5 88 8.0 89 4.0 90 3.0 91 2.5 92 2.0 93 3.0 94 6.0 95 7.5 96 6.5 97 5.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21313066232308261 2 0.008651674036534189 3 5.823242139974935E-4 4 0.002620458962988721 5 0.08352193012192621 6 0.055611962436760635 7 0.027826778511737368 8 0.016596240098928565 9 0.015306807910791257 10-11 0.013227078575085925 12-13 0.015244416030720099 14-15 0.022398684945546445 16-17 0.02528950872217686 18-19 0.024353630521109462 20-21 0.01811444251399346 22-23 0.015577172724432951 24-25 0.01395498384258279 26-27 0.012873524588016017 28-29 0.015785145658003485 30-31 0.012145619320519151 32-33 0.016138699645073393 34-35 0.018592780261205685 36-37 0.019466266582201925 38-39 0.023500941493470272 40-41 0.024811170974964634 42-43 0.02246107682561761 44-45 0.020693306890268073 46-47 0.02104686087733798 48-49 0.019133509888489074 50-51 0.01636746987200098 52-53 0.013892591962511632 54-55 0.019403874702130765 56-57 0.022689847052545193 58-59 0.018634374847919792 60-61 0.02110925275740914 62-63 0.02645415715017185 64-65 0.019279090941988444 66-67 0.02291861727947278 68-69 0.016908199499284363 70-71 0.01767769935349534 72-73 0.02597581940295962 74-75 0.01607630776500223 76-77 0.014766078283507872 78-79 0.01667942927235678 80-81 0.017303348073068377 82-83 0.013913389255868684 84-85 0.017823280406994713 86-87 0.018967131541632643 88-89 0.019466266582201925 90-91 0.013559835268798778 92-93 0.013164686695014765 94-95 0.015410794377576526 96-97 0.015306807910791257 98-99 0.014682889110079658 100-101 0.018093645220636403 102-103 0.023563333373541433 104-105 0.008672471329891242 106-107 0.008922038850175883 108-109 0.016492253632143298 110-111 0.012291200374018525 112-113 0.010128281864884977 114-115 0.006571944700828855 116-117 0.004429823485052361 118-119 0.002495675202846401 120-121 0.0023916887360611342 122-123 0.001705378055278374 124-125 5.199323339263335E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2404159.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.09632858858586 #Duplication Level Percentage of deduplicated Percentage of total 1 70.03984766651766 32.98619679996814 2 14.073739533306881 13.256429230615838 3 5.806885422751375 8.204489518385042 4 3.087877728402706 5.817108165529198 5 1.7528753246771043 4.127699613290852 6 1.149706250895454 3.2488166023514067 7 0.7907268407073861 2.6068231779638613 8 0.5648370806551319 2.1281402199641337 9 0.4242923012098141 1.7984348671846186 >10 2.151921969057288 17.998694141836992 >50 0.11889894978370612 3.771206303173629 >100 0.03662475925843564 2.866067834129413 >500 0.0010597036662078792 0.31521157045028336 >1k 6.181604719535155E-4 0.6383252649774506 >5k 8.830863885050223E-5 0.2363566901789713 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5683 0.23638203629626825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4483 0.18646853223934023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3403 0.14154637858810504 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.159458671410668E-5 7 0.0 0.0 0.0 0.0 4.159458671410668E-5 8 0.0 0.0 0.0 0.0 4.159458671410668E-5 9 0.0 0.0 0.0 0.0 4.159458671410668E-5 10-11 0.0 0.0 0.0 0.0 4.159458671410668E-5 12-13 0.0 0.0 0.0 0.0 2.495675202846401E-4 14-15 0.0 0.0 0.0 0.0 4.159458671410668E-4 16-17 0.0 0.0 0.0 0.0 5.199323339263335E-4 18-19 0.0 0.0 0.0 0.0 5.823242139974935E-4 20-21 0.0 0.0 0.0 4.159458671410668E-5 7.487025608539202E-4 22-23 2.079729335705334E-5 0.0 0.0 1.8717564021348006E-4 7.902971475680269E-4 24-25 1.039864667852667E-4 0.0 0.0 3.9514857378401347E-4 7.902971475680269E-4 26-27 1.2478376014232005E-4 0.0 0.0 0.001102256547923827 8.942836143532936E-4 28-29 1.2478376014232005E-4 0.0 0.0 0.004596201831908788 9.566754944244537E-4 30-31 1.2478376014232005E-4 0.0 0.0 0.01744892912656775 9.566754944244537E-4 32-33 1.2478376014232005E-4 0.0 0.0 0.04814573412157848 9.566754944244537E-4 34-35 1.2478376014232005E-4 0.0 0.0 0.09793445441836418 9.982700811385604E-4 36-37 1.2478376014232005E-4 0.0 0.0 0.1870300591599807 9.982700811385604E-4 38-39 1.2478376014232005E-4 0.0 0.0 0.3270166407463067 0.0011230538412808802 40-41 1.2478376014232005E-4 0.0 0.0 0.48147813850914184 0.001164648427994987 42-43 1.2478376014232005E-4 0.0 0.0 0.630095596838645 0.0011854457213520404 44-45 1.2478376014232005E-4 0.0 0.0 0.8077252794012376 0.001227040308066147 46-47 1.4558105349937338E-4 0.0 0.0 1.0243914815950192 0.0012478376014232004 48-49 1.6637834685642673E-4 0.0 0.0 1.2530369247624638 0.0012478376014232004 50-51 1.6637834685642673E-4 0.0 0.0 1.4923305821287194 0.0013726213615655204 52-53 1.6637834685642673E-4 0.0 0.0 1.7281094969176332 0.0014558105349937339 54-55 1.6637834685642673E-4 0.0 0.0 1.9878884882405865 0.0014974051217078405 56-57 1.6637834685642673E-4 0.0 0.0 2.2932343493088436 0.0014974051217078405 58-59 1.6637834685642673E-4 0.0 0.0 2.629193826198683 0.0014974051217078405 60-61 1.6637834685642673E-4 0.0 0.0 2.9796698138517463 0.0014974051217078405 62-63 1.6637834685642673E-4 0.0 0.0 3.3304785581985215 0.0015805942951360539 64-65 1.6637834685642673E-4 0.0 0.0 3.7006911772474282 0.0015805942951360539 66-67 1.6637834685642673E-4 0.0 0.0 4.120422151779479 0.0015805942951360539 68-69 1.6637834685642673E-4 0.0 0.0 4.595806683335004 0.0015805942951360539 70-71 1.6637834685642673E-4 0.0 0.0 5.109167072560509 0.0015805942951360539 72-73 1.6637834685642673E-4 0.0 0.0 5.61175446382706 0.0016221888818501605 74-75 1.6637834685642673E-4 0.0 0.0 6.115236138707964 0.001663783468564267 76-77 1.6637834685642673E-4 0.0 0.0 6.667674642151372 0.001663783468564267 78-79 1.6637834685642673E-4 0.0 0.0 7.292508523770682 0.0016845807619213205 80-81 1.6637834685642673E-4 0.0 0.0 7.9568988573551085 0.0017261753486354273 82-83 1.6637834685642673E-4 0.0 0.0 8.636554404263613 0.0018093645220636405 84-85 1.6637834685642673E-4 0.0 0.0 9.303294831997384 0.001830161815420694 86-87 1.6637834685642673E-4 0.0 0.0 9.99823638952332 0.0019133509888489073 88-89 2.0797293357053339E-4 0.0 0.0 10.760020447898828 0.0019965401622771208 90-91 2.0797293357053339E-4 0.0 0.0 11.577853211871595 0.0020173374556341737 92-93 2.0797293357053339E-4 0.0 0.0 12.414070783171994 0.002162918509133547 94-95 2.0797293357053339E-4 0.0 0.0 13.233359357679754 0.002162918509133547 96-97 2.0797293357053339E-4 0.0 0.0 14.066457334976597 0.002454080616132294 98-99 2.0797293357053339E-4 0.0 0.0 14.959950652182322 0.002703648136416934 100-101 2.0797293357053339E-4 0.0 0.0 15.929208509087793 0.002703648136416934 102-103 2.0797293357053339E-4 0.0 0.0 16.90252183819789 0.0027244454297739875 104-105 2.0797293357053339E-4 0.0 0.0 17.86782820936552 0.0027868373098451477 106-107 2.0797293357053339E-4 0.0 0.0 18.833862485800648 0.002828431896559254 108-109 2.0797293357053339E-4 0.0 0.0 19.840077133001603 0.002828431896559254 110-111 2.0797293357053339E-4 0.0 4.159458671410668E-5 20.902694039786887 0.002870026483273361 112-113 2.0797293357053339E-4 0.0 4.159458671410668E-5 21.9917859010157 0.0029116210699874677 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5155 0.0 46.87105 1 GTATCAA 7885 0.0 41.46266 1 TCAACGC 9490 0.0 33.820286 4 CAACGCA 9680 0.0 33.094944 5 ATCAACG 9930 0.0 32.3217 3 TATCAAC 10050 0.0 32.172775 2 AACGCAG 10050 0.0 31.917833 6 GTACATG 9815 0.0 30.391914 1 TACATGG 9965 0.0 29.817965 2 CATGGGG 6720 0.0 28.887041 4 ACATGGG 10365 0.0 28.09275 3 ACGCAGA 11480 0.0 27.833689 7 CGCAGAG 12060 0.0 26.441349 8 GTATAGA 745 0.0 25.625483 1 GCAGAGT 13245 0.0 23.76375 9 GAGTACT 7385 0.0 23.446653 12-13 AGTACTT 7705 0.0 22.511492 12-13 CAGAGTA 12600 0.0 21.770517 10-11 AGAGTAC 11960 0.0 21.592201 10-11 TACCGTA 250 5.280672E-7 21.421055 7 >>END_MODULE