FastQCFastQC Report
Mon 15 Aug 2016
SRR2920926_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920926_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5055379
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89050.038.4145971
GTACATG188050.032.9910161
TCAACGC100950.032.6290664
CAACGCA105150.031.38215
TACATGG193200.031.232712
ACATGGG186150.031.1995933
AACGCAG108200.030.5875766
CATGGGG133650.030.4395034
TATCAAC110550.030.3359072
GGTATCA60850.030.0674131
ATCAACG110450.029.7161983
ACGCAGA125600.026.252747
CGCAGAG132850.024.5488158
ATGGGGA73400.021.8291955
GCAGAGT153200.020.6657669
ATGGGGG73300.019.5024325
AGAGTAC127950.019.2534810-11
GTATATA20700.018.4258651
GAGTACA129750.018.3726121
GTATAGA14000.018.3045371