FastQCFastQC Report
Mon 15 Aug 2016
SRR2920926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5055379
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71380.1411961397948601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59160.11702386705329117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA118650.048.1749231
GGTATCA71100.045.9869081
TCAACGC144750.038.7558944
CAACGCA147900.037.7693985
TATCAAC152550.037.2040672
ATCAACG153650.036.7435533
AACGCAG155550.036.0071376
GTACATG174300.031.835291
ACATGGG170700.030.6662583
ACGCAGA181650.030.6602867
TACATGG183600.029.8417912
CGCAGAG190650.029.0536928
CATGGGG130150.028.7813994
GCAGAGT212550.025.47419
AGAGTAC186650.023.5111610-11
CAGAGTA199900.021.40208810-11
TATACCG5350.021.1497525
GAGTACT117250.020.90857312-13
AGTACTT124150.020.6092312-13
ATGGGGA74200.020.305895
Produced by FastQC (version 0.11.5)