FastQCFastQC Report
Mon 15 Aug 2016
SRR2920925_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920925_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3922613
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80950.032.092441
GTACATG143950.032.0791741
TACATGG147050.030.4187682
ACATGGG142550.030.3762343
CATGGGG96350.028.9919054
TCAACGC88350.028.3138144
CAACGCA89750.027.7394245
AACGCAG90100.027.681166
ATCAACG94500.026.59793
GGTATCA60400.026.3394991
TATCAAC99700.025.5690942
ACGCAGA105750.023.3573727
CGCAGAG108550.022.4787148
TTAGCGC2806.6122084E-821.2714754
ATGGGGA52900.019.0277165
GCAGAGT131300.018.038829
CTTAGCG3706.367554E-817.7075183
AGAGTAC107100.017.66849910-11
ATGGGGC47900.017.4079725
TACACCG5555.456968E-1217.1704885
Produced by FastQC (version 0.11.5)