FastQCFastQC Report
Mon 15 Aug 2016
SRR2920925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3922613
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72530.18490225775522592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60480.15418293877066128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41020.1045731506013976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77900.046.4843521
GTATCAA117650.041.2243841
TCAACGC142650.033.811514
CAACGCA144250.033.3539165
ATCAACG145250.033.1652833
AACGCAG149500.032.404246
TATCAAC151100.032.001112
GTACATG149450.028.2613491
ACGCAGA171800.028.1241557
CGCAGAG177400.027.131588
TACATGG152700.026.7131422
ACATGGG149750.025.7269763
CATGGGG98800.025.0116564
GAGTACT106550.024.62720512-13
GCAGAGT203200.023.131019
GTACTTT118550.022.0339814-15
CAGAGTA188850.021.72368210-11
AGTACTT114900.021.69821212-13
AGAGTAC179150.021.6544410-11
ACTTTTT147300.018.11656416-17
Produced by FastQC (version 0.11.5)