FastQCFastQC Report
Mon 15 Aug 2016
SRR2920924_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920924_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4125174
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89200.031.3884771
GTACATG145700.030.9100321
CATGGGG93350.029.4692974
TACATGG149800.029.0979582
GGTATCA66100.028.1183831
ACATGGG148350.028.017613
TCAACGC98050.027.0243034
CAACGCA100050.026.4840895
AACGCAG101050.026.3856456
TATCAAC105800.025.6650662
ATCAACG104050.025.6388843
ACGCAGA115000.023.0794877
CGCAGAG119500.022.1595128
ATGGGGG52450.019.2994775
ATGGGGC38550.019.1531165
GCAGAGT134750.018.8567629
GTGTAGC28300.018.7344441
AGAGTAC115450.017.16295210-11
GGACCGT7400.016.8893766
CAGAGTA127000.016.46885310-11
Produced by FastQC (version 0.11.5)