FastQCFastQC Report
Mon 15 Aug 2016
SRR2920924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4125174
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65390.15851452569031027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55040.13342467493492396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115350.042.1956561
GGTATCA77250.041.2323341
TCAACGC140900.033.6779864
ATCAACG142900.033.3316353
CAACGCA143700.033.021775
TATCAAC145600.032.8782962
AACGCAG148500.032.1003656
ACGCAGA167700.028.2088457
GTACATG144950.027.9824561
CGCAGAG171300.027.5418688
TACATGG144250.027.1596852
CATGGGG94500.026.0835884
ACATGGG146400.025.8244723
GCAGAGT188850.024.1971329
TACCGTA3404.3655746E-1122.7517957
AGAGTAC168500.022.56532910-11
GAGTACT103950.022.41058712-13
AGTACTT112050.021.00296412-13
CAGAGTA181400.020.71461310-11
GTACTTT118100.019.75080514-15
Produced by FastQC (version 0.11.5)