##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920924_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4125174 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.934388464583556 31.0 31.0 34.0 27.0 34.0 2 31.02484889122253 31.0 31.0 34.0 27.0 34.0 3 31.11831670615591 33.0 31.0 34.0 27.0 34.0 4 34.667684563123885 37.0 35.0 37.0 32.0 37.0 5 34.34622054730298 37.0 35.0 37.0 30.0 37.0 6 34.05647131490696 37.0 35.0 37.0 30.0 37.0 7 33.94347050572897 37.0 35.0 37.0 29.0 37.0 8 33.9234820155465 37.0 35.0 37.0 29.0 37.0 9 35.44221358905103 39.0 35.0 39.0 29.0 39.0 10-11 35.40423410018583 39.0 35.0 39.0 29.5 39.0 12-13 35.28522445356245 39.0 35.0 39.0 29.0 39.0 14-15 36.46235080023291 40.0 36.0 41.0 29.0 41.0 16-17 36.46362904934435 40.0 36.0 41.0 29.0 41.0 18-19 36.49475549395007 40.0 36.5 41.0 29.0 41.0 20-21 36.488965677569 40.0 36.0 41.0 29.0 41.0 22-23 36.447891410156274 40.0 36.0 41.0 29.0 41.0 24-25 36.43800479688856 40.0 36.0 41.0 29.0 41.0 26-27 36.30573510353745 40.0 36.0 41.0 28.0 41.0 28-29 36.20603361215794 40.0 36.0 41.0 28.0 41.0 30-31 36.06605939531278 40.0 36.0 41.0 27.0 41.0 32-33 35.92499576987541 40.0 35.5 41.0 27.0 41.0 34-35 35.81233434032116 40.0 35.0 41.0 27.0 41.0 36-37 35.68283507556288 40.0 35.0 41.0 26.0 41.0 38-39 35.56500004605866 40.0 35.0 41.0 26.0 41.0 40-41 35.42948551988353 40.0 35.0 41.0 25.5 41.0 42-43 35.286069145204536 39.5 35.0 41.0 25.0 41.0 44-45 35.09610212805569 39.0 35.0 41.0 24.0 41.0 46-47 34.900929148685606 39.0 35.0 41.0 23.5 41.0 48-49 34.65746196402867 39.0 34.0 41.0 22.5 41.0 50-51 34.47903033908388 39.0 34.0 41.0 22.0 41.0 52-53 34.28213876554056 39.0 34.0 41.0 21.0 41.0 54-55 34.07909157286456 38.5 34.0 41.0 20.0 41.0 56-57 33.83108203435782 38.0 33.0 40.5 19.0 41.0 58-59 33.60578050768282 38.0 33.0 40.0 18.0 41.0 60-61 33.34463479601103 37.0 33.0 40.0 17.0 41.0 62-63 33.053814820902105 37.0 33.0 40.0 13.5 41.0 64-65 32.74867581343236 36.5 32.0 40.0 13.0 41.0 66-67 32.432675082311675 36.0 32.0 39.0 10.0 41.0 68-69 32.074228990098355 35.5 32.0 39.0 8.0 41.0 70-71 31.744544836169332 35.0 31.0 38.5 7.5 40.0 72-73 31.380638125809966 35.0 31.0 37.5 7.0 40.0 74-75 31.051665214606704 35.0 31.0 37.0 4.0 39.0 76-77 30.84233949889144 35.0 31.0 36.5 2.0 39.0 78-79 30.691687793048246 35.0 31.0 36.0 2.0 39.0 80-81 30.435778224142787 35.0 31.0 36.0 2.0 37.0 82-83 30.21071765700065 35.0 31.0 35.0 2.0 37.0 84-85 29.964969356444115 35.0 31.0 35.0 2.0 36.5 86-87 29.79262632315631 35.0 31.0 35.0 2.0 36.0 88-89 29.641917771226137 35.0 30.5 35.0 2.0 36.0 90-91 29.48103413334807 34.5 30.0 35.0 2.0 36.0 92-93 29.35974943117551 34.0 30.0 35.0 2.0 35.0 94-95 29.27015418016307 34.0 30.0 35.0 2.0 35.0 96-97 29.203194337984286 34.0 30.0 35.0 2.0 35.0 98-99 29.102488767746525 34.0 30.0 35.0 2.0 35.0 100-101 29.004292303791306 34.0 30.0 35.0 2.0 35.0 102-103 27.594879997789185 32.5 26.5 34.0 2.0 35.0 104-105 28.665968514297823 34.0 29.0 35.0 2.0 35.0 106-107 28.849872756882498 34.0 29.5 35.0 2.0 35.0 108-109 28.948080977917538 34.0 30.0 35.0 2.0 35.0 110-111 29.122988024262735 35.0 31.0 35.0 2.0 35.0 112-113 29.105015085424277 35.0 31.0 35.0 2.0 35.0 114-115 29.02631149619386 35.0 31.0 35.0 2.0 35.0 116-117 28.91447124412207 35.0 30.0 35.0 2.0 35.0 118-119 28.812671174597725 35.0 30.0 35.0 2.0 35.0 120-121 28.632137335297855 35.0 30.0 35.0 2.0 35.0 122-123 28.446466985392618 35.0 30.0 35.0 2.0 35.0 124-125 27.71810486054649 34.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 92070.0 3 35243.0 4 14800.0 5 11947.0 6 13485.0 7 15816.0 8 17771.0 9 19606.0 10 21885.0 11 24412.0 12 25277.0 13 28286.0 14 32714.0 15 29314.0 16 19942.0 17 17486.0 18 16329.0 19 15654.0 20 15229.0 21 15505.0 22 17135.0 23 20385.0 24 27622.0 25 37230.0 26 44825.0 27 54125.0 28 64816.0 29 77398.0 30 92458.0 31 112403.0 32 138770.0 33 176924.0 34 237315.0 35 350838.0 36 643582.0 37 1111886.0 38 433849.0 39 842.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20634120972586 18.748367974578418 12.291486359485981 25.75380445620974 2 16.403598195810996 20.853413885855105 37.46492292437655 25.27806499395735 3 18.953474221810872 24.61415985924404 29.86402447035917 26.56834144858592 4 13.294861585341463 16.7043990395396 36.117989723897935 33.882749651221 5 13.640309230480346 37.49633064158095 34.744978983370586 14.118381144568112 6 32.75487759002025 36.52963905554926 16.834267566563966 13.881215787866521 7 27.879938391645627 32.19526317882426 21.656204626639987 18.268593802890123 8 26.452718797922188 35.10290275849947 19.990859553524345 18.453518890054006 9 26.503079681376324 14.829287097098758 19.478642249034152 39.188990972490764 10-11 25.295512687480482 25.731350868039733 28.02019833704108 20.952938107438708 12-13 25.853724233471382 23.298576310043508 27.972930446677207 22.8747690098079 14-15 23.115353519458715 24.601823639668414 25.41903427086237 26.863788570010506 16-17 23.09232648564138 27.437483815090008 26.15916689054782 23.311022808720793 18-19 22.970492085497117 26.57701766328644 27.89911819956804 22.5533720516484 20-21 23.761471115216672 25.79781985166651 27.715741825049882 22.724967208066936 22-23 23.398320755526665 25.70177227183385 27.546463372264853 23.353443600374632 24-25 23.158441128969397 25.839144007955074 27.737249902386 23.26516496068953 26-27 23.1080498728087 26.01493323441687 27.730903576448423 23.146113316326005 28-29 23.085457249191403 26.08204273171581 27.710736170549616 23.12176384854317 30-31 23.236376754568113 25.87738406340483 27.640237562043406 23.246001619983645 32-33 23.0397388532839 26.023886564880094 27.65962966393168 23.27674491790433 34-35 23.205751255438294 26.0815971851803 27.709896771840942 23.002754787540468 36-37 23.366428935982437 25.87386332364711 27.505646255287115 23.254061485083337 38-39 23.218422799028485 26.10086461131043 27.532605077103135 23.148107512557946 40-41 23.311579302395984 25.974436599048573 27.548905349658536 23.16507874889691 42-43 23.264999132582915 25.943105440456936 27.451150355083335 23.34074507187681 44-45 23.28147522242656 25.989678486177976 27.377033480061925 23.35181281133354 46-47 23.33038623049545 26.0545227360316 27.153048954275537 23.462042079197417 48-49 23.229033403480308 26.241482016144996 27.039518498955793 23.4899660814189 50-51 23.26512121715756 26.42039073011537 26.864619878893762 23.44986817383331 52-53 23.322717090607227 26.307124633105634 26.789237741003518 23.580920535283624 54-55 23.19908971928809 26.26065538077636 26.83891542177099 23.701339478164563 56-57 23.156428976517095 26.38044001930985 26.727879196541295 23.73525180763176 58-59 23.028597904745418 26.25599987198333 26.887997298072502 23.82740492519875 60-61 23.169184985182014 26.275737511028936 26.97161196873517 23.583465535053882 62-63 22.996338131443185 26.24348831412441 26.981257110600943 23.778916443831466 64-65 22.853091047247556 26.238948336081386 26.970563608037995 23.93739700863306 66-67 22.921706155161996 26.33311935775807 26.792263661552468 23.952910825527468 68-69 23.010497045550395 26.111362923612237 26.8572034006689 24.020936630168467 70-71 22.83164125026488 25.952915042834263 27.038621874834828 24.17682183206603 72-73 22.84847175506182 25.997197522530385 26.943488301268996 24.210842421138796 74-75 22.71883548616329 26.079730473787453 26.832099629270534 24.36933441077872 76-77 22.67491773219426 25.92573556134246 26.95903811218388 24.440308594279404 78-79 22.696340749510338 26.017442759089874 26.900381971766652 24.385834519633136 80-81 22.560963339198306 26.042838782132176 26.835264603816185 24.560933274853333 82-83 22.57649113759986 25.916585053572494 26.899603105188824 24.60732070363882 84-85 22.441349749491668 25.97553626627722 26.814871620616586 24.76824236361452 86-87 22.347153780942836 25.840369471985625 26.982476944672186 24.829999802399357 88-89 22.35782267881066 25.735324692107692 26.896351237806414 25.01050139127523 90-91 22.239390046309484 25.775467489170573 26.934574427204694 25.050568037315244 92-93 22.187842225758143 25.782071849437997 26.943466517062163 25.086619407741694 94-95 22.043247725239475 25.657819769626705 27.036907731882533 25.26202477325129 96-97 22.047735579091714 25.457444044847765 27.094364013945192 25.400456362115325 98-99 21.92955904060763 25.55227054829019 26.96940320608035 25.548767205021832 100-101 21.777141734918025 25.54844755434651 27.038860844813623 25.635549865921842 102-103 21.664878663290374 25.433397349067278 27.06580462637236 25.835919361269994 104-105 21.500375588459296 25.4883043923546 27.00566479763376 26.00565522155234 106-107 21.358321237941073 25.561893295972776 26.987969741437805 26.09181572464834 108-109 21.321541236839085 25.51591735430392 26.986409857705183 26.17613155115181 110-111 21.148048998448857 25.669902940867 26.988834587322913 26.193213473361226 112-113 21.058975765418218 25.6666191894734 26.99517247858223 26.279232566526154 114-115 20.945172709933495 25.71218869756473 26.898785003057647 26.443853589444128 116-117 20.733402658493425 25.650353678316307 27.03735788958342 26.578885773606846 118-119 20.74101889492002 25.64430918501642 26.95571500847136 26.658956911592195 120-121 20.670219335804806 25.78075389069391 26.904323383799174 26.64470338970211 122-123 20.51513405368741 25.7553209000216 26.837258045318162 26.892287000972825 124-125 20.35365986691393 25.79741342739052 26.877289310691733 26.971637395003818 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 175.0 1 169.5 2 277.0 3 431.0 4 325.5 5 233.5 6 382.5 7 781.5 8 1271.5 9 1398.0 10 1199.0 11 1032.0 12 1114.0 13 1266.5 14 1382.5 15 1600.5 16 1841.0 17 2128.0 18 2493.5 19 2906.5 20 3388.5 21 4007.5 22 5044.0 23 6461.5 24 8064.0 25 10210.0 26 13614.5 27 17821.0 28 21750.5 29 26645.0 30 32737.5 31 39662.5 32 47611.0 33 55236.5 34 62758.5 35 70106.5 36 78652.0 37 87945.0 38 96719.0 39 105235.0 40 113174.5 41 121337.0 42 130034.0 43 139467.5 44 148029.0 45 156378.0 46 163856.0 47 167177.0 48 170204.5 49 172624.0 50 173889.5 51 172437.5 52 166175.0 53 161437.5 54 157004.5 55 148512.5 56 136203.0 57 123108.0 58 111434.0 59 98918.0 60 83406.5 61 68865.5 62 55861.0 63 42088.5 64 30737.5 65 23126.0 66 17803.5 67 13999.0 68 10788.5 69 7941.0 70 5989.5 71 4544.0 72 3395.5 73 2592.5 74 2006.0 75 1505.5 76 1067.0 77 702.5 78 433.0 79 267.5 80 173.0 81 137.0 82 110.5 83 68.5 84 34.0 85 24.0 86 21.5 87 13.0 88 10.5 89 10.0 90 7.0 91 5.5 92 6.0 93 6.0 94 6.5 95 10.0 96 10.0 97 6.0 98 2.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2025853939736845 2 0.007975421157992366 3 4.848280339205086E-4 4 0.0016726567170257545 5 0.07757248542728137 6 0.05296746270581556 7 0.025647402994394903 8 0.015732669700720504 9 0.013550943548078216 10-11 0.011587390010700154 12-13 0.01309035691585373 14-15 0.020350656723813348 16-17 0.023320228431576464 18-19 0.02282327969680794 20-21 0.016581118760081393 22-23 0.014084254385390772 24-25 0.012350994164124957 26-27 0.011672234916636243 28-29 0.014047892282846735 30-31 0.010690458147947213 32-33 0.014447875410831154 34-35 0.017405326417746256 36-37 0.0183992238872833 38-39 0.021647571714550708 40-41 0.02321114212394435 42-43 0.02085972615942988 44-45 0.01888405192120381 46-47 0.019017379630531947 48-49 0.017902275152514777 50-51 0.0148720999405116 52-53 0.012593408181085211 54-55 0.017744706041490612 56-57 0.02072639845010174 58-59 0.01723563660587408 60-61 0.019271914348340215 62-63 0.025453471780826697 64-65 0.017902275152514777 66-67 0.021126381578086162 68-69 0.015332686572736082 70-71 0.016290221939729087 72-73 0.023417194038360564 74-75 0.014617565222703332 76-77 0.012969149907373603 78-79 0.015865997410048644 80-81 0.016653842965169467 82-83 0.012835822198045466 84-85 0.016011445820224793 86-87 0.016811412076193633 88-89 0.017465929921986322 90-91 0.012920667103981553 92-93 0.012629770283629249 94-95 0.013672150556558342 96-97 0.014508478915071218 98-99 0.013150960420093796 100-101 0.017308360810962157 102-103 0.022483900073063584 104-105 0.007599679431703973 106-107 0.007878455551208264 108-109 0.014629685923551346 110-111 0.011441941600524003 112-113 0.009369301755513829 114-115 0.006460333551990776 116-117 0.004084676185780284 118-119 0.0023514159645144665 120-121 0.002581709280626708 122-123 0.0017696223238098563 124-125 5.817936407046103E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4125174.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.72321177589664 #Duplication Level Percentage of deduplicated Percentage of total 1 69.9690049211258 34.79083649441892 2 14.297143070329202 14.217997453525438 3 6.1095433282197495 9.11358350289261 4 3.0807107753053993 6.127313372031883 5 1.840700553559249 4.576277172031836 6 1.1438855723173333 3.4126598735836495 7 0.7435981030466764 2.588186016776143 8 0.5267193371135782 2.095214171660667 9 0.3847302627426734 1.7217021897855276 >10 1.7931428663928475 15.213325011553147 >50 0.0786200106466597 2.659666080694446 >100 0.030887122561414744 2.599114397879257 >500 9.733901283104614E-4 0.3391877773250445 >1k 2.433475078835404E-4 0.2534954120787476 >5k 9.733900315341617E-5 0.2914410737627071 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6539 0.15851452569031027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5504 0.13342467493492396 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.2120700848012714E-5 8.4844905936089E-5 14-15 0.0 0.0 0.0 4.848280339205086E-5 1.0908630763211444E-4 16-17 0.0 0.0 0.0 4.848280339205086E-5 1.2120700848012714E-4 18-19 0.0 0.0 0.0 7.272420508807629E-5 1.4544841017615257E-4 20-21 0.0 0.0 0.0 1.4544841017615257E-4 1.69689811872178E-4 22-23 0.0 0.0 0.0 3.0301752120031787E-4 1.9393121356820343E-4 24-25 0.0 0.0 0.0 4.848280339205085E-4 1.9393121356820343E-4 26-27 0.0 0.0 0.0 8.969318627529408E-4 2.0605191441621615E-4 28-29 0.0 0.0 0.0 0.0033210720323554833 2.1817261526422884E-4 30-31 0.0 0.0 0.0 0.011987373138684573 2.3029331611224157E-4 32-33 0.0 0.0 0.0 0.034883377040580595 2.424140169602543E-4 34-35 0.0 0.0 0.0 0.06966978847437708 2.424140169602543E-4 36-37 2.424140169602543E-5 0.0 0.0 0.13115810387634558 2.424140169602543E-4 38-39 2.424140169602543E-5 0.0 0.0 0.23055997153089786 2.54534717808267E-4 40-41 2.424140169602543E-5 0.0 0.0 0.34516119804885803 2.666554186562797E-4 42-43 2.424140169602543E-5 0.0 0.0 0.45676861145735914 2.666554186562797E-4 44-45 2.424140169602543E-5 0.0 0.0 0.5924962195534056 2.666554186562797E-4 46-47 2.424140169602543E-5 0.0 0.0 0.7573135096846824 2.666554186562797E-4 48-49 2.424140169602543E-5 0.0 0.0 0.9310637563409446 2.666554186562797E-4 50-51 2.424140169602543E-5 0.0 0.0 1.1165347207172351 2.9089682035230514E-4 52-53 2.424140169602543E-5 0.0 0.0 1.3003936318807399 3.636210254403814E-4 54-55 2.424140169602543E-5 0.0 0.0 1.5096817734233756 3.636210254403814E-4 56-57 2.424140169602543E-5 0.0 0.0 1.7578773646881318 3.7574172628839414E-4 58-59 4.848280339205086E-5 0.0 0.0 2.026823595804686 3.8786242713640686E-4 60-61 4.848280339205086E-5 0.0 0.0 2.3122903421770813 3.8786242713640686E-4 62-63 4.848280339205086E-5 0.0 0.0 2.600399401334344 4.121038288324323E-4 64-65 6.060350424006357E-5 0.0 0.0 2.907041012088217 4.363452305284577E-4 66-67 7.272420508807629E-5 0.0 0.0 3.267268241291155 4.363452305284577E-4 68-69 7.272420508807629E-5 0.0 0.0 3.6722814601275 4.363452305284577E-4 70-71 7.272420508807629E-5 0.0 0.0 4.106978275340628 4.7270733307249585E-4 72-73 7.272420508807629E-5 0.0 0.0 4.541250866024075 4.848280339205086E-4 74-75 7.272420508807629E-5 0.0 0.0 4.984310964822333 4.969487347685213E-4 76-77 7.272420508807629E-5 0.0 0.0 5.476144763832993 5.211901364645466E-4 78-79 7.272420508807629E-5 0.0 0.0 6.023830752351294 5.333108373125594E-4 80-81 7.272420508807629E-5 0.0 0.0 6.6066667733288345 5.333108373125594E-4 82-83 7.272420508807629E-5 0.0 0.0 7.206483896194439 5.817936407046103E-4 84-85 7.272420508807629E-5 0.0 0.0 7.804337465522666 5.817936407046103E-4 86-87 7.272420508807629E-5 0.0 0.0 8.43771680903642 5.939143415526231E-4 88-89 7.272420508807629E-5 0.0 0.0 9.130730000722394 6.060350424006357E-4 90-91 7.272420508807629E-5 0.0 0.0 9.872541134022468 6.181557432486484E-4 92-93 7.272420508807629E-5 0.0 0.0 10.63882396233468 7.272420508807628E-4 94-95 7.272420508807629E-5 0.0 0.0 11.3943799703964 7.272420508807628E-4 96-97 7.272420508807629E-5 0.0 0.0 12.171486584565887 8.484490593608899E-4 98-99 7.272420508807629E-5 0.0 0.0 12.99758507156304 9.454146661449917E-4 100-101 7.272420508807629E-5 0.0 0.0 13.8916685696167 9.454146661449917E-4 102-103 7.272420508807629E-5 0.0 0.0 14.808102640034093 9.696560678410171E-4 104-105 7.272420508807629E-5 0.0 0.0 15.711422112133937 9.938974695370426E-4 106-107 7.272420508807629E-5 0.0 0.0 16.615565791891445 9.938974695370426E-4 108-109 7.272420508807629E-5 0.0 0.0 17.562653114753463 0.001018138871233068 110-111 7.272420508807629E-5 0.0 0.0 18.55390827150564 0.001018138871233068 112-113 7.272420508807629E-5 0.0 0.0 19.57525912846343 0.001018138871233068 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11535 0.0 42.195656 1 GGTATCA 7725 0.0 41.232334 1 TCAACGC 14090 0.0 33.677986 4 ATCAACG 14290 0.0 33.331635 3 CAACGCA 14370 0.0 33.02177 5 TATCAAC 14560 0.0 32.878296 2 AACGCAG 14850 0.0 32.100365 6 ACGCAGA 16770 0.0 28.208845 7 GTACATG 14495 0.0 27.982456 1 CGCAGAG 17130 0.0 27.541868 8 TACATGG 14425 0.0 27.159685 2 CATGGGG 9450 0.0 26.083588 4 ACATGGG 14640 0.0 25.824472 3 GCAGAGT 18885 0.0 24.197132 9 TACCGTA 340 4.3655746E-11 22.751795 7 AGAGTAC 16850 0.0 22.565329 10-11 GAGTACT 10395 0.0 22.410587 12-13 AGTACTT 11205 0.0 21.002964 12-13 CAGAGTA 18140 0.0 20.714613 10-11 GTACTTT 11810 0.0 19.750805 14-15 >>END_MODULE