FastQCFastQC Report
Mon 15 Aug 2016
SRR2920923_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920923_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3708401
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102100.033.1516081
GTACATG171200.030.0044381
TACATGG169450.029.7040182
CATGGGG105150.028.8319074
ACATGGG174500.028.093413
AACGCAG117050.028.0189446
TCAACGC117400.027.903554
CAACGCA118300.027.6912635
ATCAACG122450.026.8035853
TATCAAC124250.026.798812
ACGCAGA132600.024.5516267
GGTATCA74550.024.3800261
CGCAGAG135650.023.953468
CGTACAC4400.023.0142233
GTATCGC3404.3655746E-1122.7581026
ATGGGGA48000.021.094665
GTATAGG8550.020.9091851
GCAGAGT153600.019.9144049
ATGGGGG55700.019.354785
ATGGGGC47300.017.881015
Produced by FastQC (version 0.11.5)