FastQCFastQC Report
Mon 15 Aug 2016
SRR2920923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3708401
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88710.2392136125516092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70790.19089089879977922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49110.13242904421609206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93050.040.3344231
GTATCAA141500.040.3128051
TCAACGC175300.031.897944
TATCAAC177750.031.7937242
ATCAACG177400.031.755323
CAACGCA175800.031.7394715
AACGCAG180100.030.9641176
ACGCAGA202250.027.4475047
CATGGGG102500.026.434224
CGCAGAG209950.026.411098
GTACATG169700.026.33551
TACATGG169150.025.629742
ACATGGG173600.024.2177583
GCAGAGT225800.024.1892669
GAGTACT114950.024.1473812-13
AGTACTT120800.023.1257612-13
GTATAGG6750.022.0992661
GTACTTT125250.022.01923814-15
AGAGTAC203700.021.90774210-11
CAGAGTA211950.021.1392210-11
Produced by FastQC (version 0.11.5)