FastQCFastQC Report
Mon 15 Aug 2016
SRR2920922_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920922_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909140
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74600.034.7449151
GTACATG131850.032.3122141
TACATGG135400.031.0114442
TCAACGC81800.029.9250224
CAACGCA82500.029.7433055
ACATGGG139200.029.0096193
ATCAACG84850.028.7093263
AACGCAG85600.028.5080016
CATGGGG100000.028.349994
TATCAAC88200.028.0915872
GGTATCA51250.027.9034671
ACGCAGA100450.023.8173737
CGCAGAG104900.022.5216088
ATGGGGA58100.020.502175
GTAATAC8000.020.1013533
GCAGAGT117250.019.286339
GTATAAT17100.018.8164841
ATGGGGG58550.018.717035
AGAGTAC100250.018.2514710-11
GTATAAG16750.017.430991
Produced by FastQC (version 0.11.5)