##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920922_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3909140 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.977136659214047 31.0 30.0 34.0 22.0 34.0 2 29.05315465805778 31.0 30.0 34.0 20.0 34.0 3 29.13047882654497 31.0 30.0 34.0 22.0 34.0 4 32.503559606460755 35.0 33.0 37.0 22.0 37.0 5 32.518880879170354 35.0 33.0 37.0 23.0 37.0 6 32.49502729500606 37.0 33.0 37.0 23.0 37.0 7 32.351713164532356 36.0 33.0 37.0 21.0 37.0 8 32.284489171531334 36.0 33.0 37.0 19.0 37.0 9 33.61480811636319 38.0 34.0 39.0 19.0 39.0 10-11 33.607424523040876 38.0 34.0 39.0 19.0 39.0 12-13 33.56303662698189 38.0 34.0 39.0 18.0 39.0 14-15 34.644922668413 39.0 34.0 41.0 18.0 41.0 16-17 34.63766518978599 39.0 34.0 41.0 18.0 41.0 18-19 34.600376553410726 39.0 34.0 41.0 18.0 41.0 20-21 34.59346275651422 39.0 34.0 41.0 18.0 41.0 22-23 34.51051996091212 39.0 34.0 41.0 18.0 41.0 24-25 34.5094802181554 39.0 34.0 41.0 18.0 41.0 26-27 34.42307668694393 39.0 34.0 41.0 18.0 41.0 28-29 34.31008341988263 39.0 34.0 41.0 17.5 41.0 30-31 34.163286681981205 39.0 33.5 41.0 16.5 41.0 32-33 34.022495868656534 39.0 33.0 41.0 16.0 41.0 34-35 33.95758530009158 39.0 33.0 41.0 15.5 41.0 36-37 33.830634231570116 39.0 33.0 41.0 14.5 41.0 38-39 33.69630199992837 39.0 33.0 41.0 10.0 41.0 40-41 33.55000984871353 38.5 33.0 41.0 9.0 41.0 42-43 33.36501864860302 38.0 32.5 41.0 8.5 41.0 44-45 33.207702461410946 38.0 32.0 40.0 8.0 41.0 46-47 32.99797679284958 38.0 32.0 40.0 4.5 41.0 48-49 32.880572709086906 38.0 32.0 40.0 2.0 41.0 50-51 32.673831712345944 38.0 32.0 40.0 2.0 41.0 52-53 32.464286134546214 38.0 31.0 40.0 2.0 41.0 54-55 32.21377195495684 37.5 31.0 40.0 2.0 41.0 56-57 31.938493504965287 37.0 31.0 40.0 2.0 41.0 58-59 31.656773996326557 36.5 30.0 40.0 2.0 41.0 60-61 31.37909271604496 36.0 30.0 40.0 2.0 41.0 62-63 31.06453260819515 36.0 30.0 39.0 2.0 41.0 64-65 30.779091564896625 35.0 29.0 39.0 2.0 41.0 66-67 30.433301442260955 35.0 29.0 39.0 2.0 40.0 68-69 30.105263689711805 35.0 29.0 38.0 2.0 40.0 70-71 29.781997574914175 35.0 29.0 37.5 2.0 40.0 72-73 29.8387903733302 35.0 29.0 37.0 2.0 39.5 74-75 29.84063962917675 35.0 30.0 37.0 2.0 39.0 76-77 29.58728991542897 35.0 29.0 36.0 2.0 39.0 78-79 29.30973065687082 35.0 29.0 36.0 2.0 38.5 80-81 29.054343538476495 35.0 29.0 35.5 2.0 37.0 82-83 28.82909936712423 34.0 29.0 35.0 2.0 37.0 84-85 28.613524330159578 34.0 29.0 35.0 2.0 36.5 86-87 28.42360276173276 34.0 29.0 35.0 2.0 36.0 88-89 28.240071473521027 34.0 29.0 35.0 2.0 36.0 90-91 28.07790127751884 34.0 29.0 35.0 2.0 35.5 92-93 27.92224811595389 34.0 28.0 35.0 2.0 35.0 94-95 27.819969609683973 34.0 28.0 35.0 2.0 35.0 96-97 27.73564607560742 34.0 27.5 35.0 2.0 35.0 98-99 27.613771443335363 34.0 27.0 35.0 2.0 35.0 100-101 27.502053137007117 34.0 27.0 35.0 2.0 35.0 102-103 26.201220345139852 32.0 24.5 34.0 2.0 35.0 104-105 27.200482586962863 34.0 26.5 35.0 2.0 35.0 106-107 27.36568900064976 34.0 27.0 35.0 2.0 35.0 108-109 27.3060863770548 34.0 27.0 35.0 2.0 35.0 110-111 27.21511329857718 34.0 27.0 35.0 2.0 35.0 112-113 27.110456136132242 34.0 26.5 35.0 2.0 35.0 114-115 27.018093237898874 34.0 25.5 35.0 2.0 35.0 116-117 26.81541285295487 34.0 25.0 35.0 2.0 35.0 118-119 26.638029336375777 34.0 25.0 35.0 2.0 35.0 120-121 26.474381449628307 34.0 25.0 35.0 2.0 35.0 122-123 26.19780207411349 34.0 24.5 35.0 2.0 35.0 124-125 24.95670978783057 32.5 19.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 214775.0 3 29756.0 4 19157.0 5 17635.0 6 19595.0 7 21337.0 8 22122.0 9 23442.0 10 24631.0 11 26014.0 12 25800.0 13 24688.0 14 24488.0 15 22174.0 16 19116.0 17 17931.0 18 17294.0 19 17045.0 20 17536.0 21 18702.0 22 22301.0 23 28835.0 24 38597.0 25 48375.0 26 54747.0 27 61074.0 28 70956.0 29 82767.0 30 97072.0 31 116095.0 32 142888.0 33 180033.0 34 240171.0 35 346084.0 36 600643.0 37 873326.0 38 281611.0 39 327.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36234912476026 18.198503835219448 11.861365817285456 25.577781222734835 2 16.265017338339455 20.227012354079225 38.37442308007608 25.133547227505247 3 19.167305788690246 24.301516288707028 29.07949260074788 27.451685321854846 4 13.214359548195755 16.046782043724825 35.82465597332069 34.91420243475873 5 14.034455658960058 37.78484034027737 34.02827513381529 14.152428866947284 6 33.48367412685479 35.86695812908172 16.783528207849304 13.865839536214189 7 28.45059609202102 32.387461606265305 21.060176689285914 18.101765612427766 8 26.470965543147308 35.65804274465692 19.625173190706043 18.24581852148973 9 26.45075233110097 15.783345441341373 18.905342179477298 38.86056004808036 10-11 25.52908539028665 26.10124294132482 27.507306712958517 20.86236495543001 12-13 25.516503457010163 23.61089172608836 27.56263822856486 23.309966588336614 14-15 22.98487192303926 25.08466462159967 24.536861447244377 27.39360200811669 16-17 23.350864327462773 27.821517438169934 25.388098372058582 23.439519862308714 18-19 23.48932450049696 27.207461361674184 26.548096227655243 22.75511791017361 20-21 24.272313509058 26.392810425727443 26.244505937555196 23.09037012765936 22-23 23.85683851747419 26.14985490753475 26.211388344328213 23.78191823066285 24-25 23.605904682259908 26.32699781921931 26.281408636628743 23.785688861892037 26-27 23.53363127676419 26.3874881679167 26.290041081889214 23.788839473429892 28-29 23.648088846485795 26.57695715787088 26.226495923957042 23.548458071686287 30-31 23.824681390034605 26.42886525525274 26.121895909438926 23.62455744527373 32-33 23.614612820169846 26.53788085819374 26.112432007608366 23.735074314028047 34-35 23.73219331283259 26.44836468709701 26.21338905439523 23.606052945675167 36-37 23.703671846040102 26.34921756440425 26.291976169557408 23.655134419998237 38-39 23.70760316852446 26.51145907356703 26.19711787558135 23.583819882327163 40-41 23.776173470961645 26.451584990764093 26.301542296052595 23.47069924222167 42-43 23.60929528377552 26.49141539982577 26.19633616888265 23.702953147516062 44-45 23.66630129664547 26.43580947037047 26.160911619534616 23.73697761344944 46-47 23.542328764545996 26.46672638586357 26.21397569994393 23.776969149646504 48-49 23.505343598201957 26.550749209479186 26.1712301102813 23.772677082037553 50-51 23.425442592100087 26.676766407257684 26.16273935476506 23.735051645877174 52-53 23.575432193539157 26.559299920315176 26.038110489292464 23.827157396853206 54-55 23.523170379267082 26.446342447999662 26.063563323342446 23.966923849390806 56-57 23.43647224749039 26.589580948643526 26.04880593677527 23.925140867090814 58-59 23.52632748266787 26.406893331961406 26.163299040212383 23.903480145158344 60-61 23.44876540547348 26.345034737390044 26.31700471429151 23.889195142844965 62-63 23.40667666647896 26.422349580468623 26.230258476376722 23.940715276675697 64-65 23.38863950603254 26.36783467938647 26.19156033128418 24.051965483296808 66-67 23.357971393434536 26.467696189720357 26.127171092264295 24.047161324580813 68-69 23.39302409665539 26.250245574067378 26.270096277833062 24.086634051444168 70-71 23.175138576334213 26.16364191097123 26.378609281286924 24.282610231407634 72-73 23.2283088707667 26.127203640181435 26.4124950282284 24.231992460823466 74-75 23.116214110814873 26.241256103402677 26.40730679346327 24.23522299231918 76-77 23.177188491802685 26.0906253835701 26.482735617793125 24.24945050683409 78-79 23.11753542165233 26.075972403688773 26.552625257354016 24.253866917304876 80-81 23.14004423974646 26.080999382117863 26.52412632643732 24.254830051698356 82-83 23.076960487878235 26.00613687339419 26.621398426426314 24.295504212301264 84-85 23.116890563418103 26.069788413922257 26.50874652546423 24.304574497195414 86-87 23.03016636401716 25.928127642370796 26.693084988819095 24.34862100479295 88-89 23.006147178291204 25.814040721904714 26.6988694019283 24.48094269787578 90-91 22.953007525051724 25.850289378796088 26.677855655895836 24.51884744025635 92-93 23.01962326022697 25.83951511205266 26.681340152805944 24.459521474914432 94-95 22.928320090798636 25.690201607943752 26.80616581937568 24.57531248188193 96-97 22.945072208146396 25.602875605786892 26.814417392045453 24.63763479402126 98-99 22.841435330334104 25.701456736825563 26.78338800980542 24.67371992303491 100-101 22.783537924320274 25.621975539169167 26.92087493486564 24.673611601644918 102-103 22.577351143068903 25.67128169902257 26.969262748197252 24.782104409711277 104-105 22.505717957110907 25.748017813256652 26.814819573084797 24.931444656547647 106-107 22.4473250438958 25.79594725337729 26.911693942636 24.845033760090914 108-109 22.383505267575103 25.792132698280007 26.950076510726923 24.874285523417964 110-111 22.37119798664504 25.913395737030083 26.83987792294955 24.87552835337533 112-113 22.363999841298483 25.945393880520797 26.877598897280446 24.813007380900274 114-115 22.28355706115307 26.042176753382158 26.804129516995186 24.870136668469595 116-117 22.141446488476245 25.95524888678982 26.977367179530496 24.925937445203434 118-119 22.294669315409525 25.89613283030768 26.891382345314874 24.91781550896792 120-121 22.216259516846804 25.942072964315958 26.886606451163715 24.95506106767352 122-123 22.16202511751639 25.998954897946412 26.875978023420988 24.963041961116215 124-125 22.189651043380636 26.01919224927115 26.742623133583777 25.04853357376444 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1944.0 1 1500.5 2 837.5 3 576.5 4 474.0 5 403.0 6 410.0 7 452.0 8 496.5 9 553.5 10 584.0 11 612.5 12 667.5 13 767.0 14 922.5 15 1007.5 16 1143.0 17 1356.5 18 1538.0 19 1799.0 20 2209.0 21 2897.0 22 3835.0 23 5076.0 24 6735.5 25 8886.5 26 11783.5 27 15346.0 28 19279.0 29 23973.5 30 29885.5 31 36572.5 32 42270.0 33 48093.0 34 54748.5 35 61572.5 36 70567.0 37 79580.5 38 87856.0 39 95143.0 40 102673.0 41 111138.5 42 119190.5 43 128411.0 44 136036.0 45 143038.0 46 150243.0 47 154819.5 48 158465.0 49 162424.0 50 165683.5 51 167232.0 52 164215.0 53 159777.5 54 154895.0 55 146127.0 56 135746.0 57 124295.5 58 112975.0 59 99844.5 60 83120.0 61 68006.0 62 55047.0 63 42398.5 64 32175.5 65 24764.0 66 19337.0 67 15288.0 68 12053.0 69 9219.0 70 6905.5 71 5253.0 72 3916.5 73 3025.0 74 2350.5 75 1688.5 76 1188.5 77 836.0 78 541.5 79 360.5 80 247.0 81 172.0 82 116.5 83 88.5 84 78.5 85 61.5 86 42.0 87 36.5 88 34.0 89 24.5 90 17.5 91 14.0 92 9.5 93 9.5 94 15.0 95 12.5 96 8.5 97 12.0 98 14.5 99 13.5 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16614907626741432 2 0.14458423080268296 3 0.17013972382672404 4 0.14302378528269644 5 0.1677862650096952 6 0.1083102677315215 7 0.1075939976567736 8 0.09626158183129793 9 0.10260568820763646 10-11 0.08915004323201522 12-13 0.07722926270228235 14-15 0.07655136423868165 16-17 0.07701182357244815 18-19 0.07373744608788634 20-21 0.06778984636006896 22-23 0.059373672981781166 24-25 0.06424686759747668 26-27 0.07463278368132122 28-29 0.06949098778759523 30-31 0.06551313076533458 32-33 0.07450487831083052 34-35 0.07105143330758172 36-37 0.07357116910624843 38-39 0.06947819725054616 40-41 0.07465836475541936 42-43 0.07421069595870192 44-45 0.06645963050696573 46-47 0.06455384048665436 48-49 0.05684114664606538 50-51 0.06239223972536159 52-53 0.06392710417124994 54-55 0.06597359009910109 56-57 0.06931192026890826 58-59 0.05714811953524305 60-61 0.06688171822958502 62-63 0.06139457783553416 64-65 0.055306282200177026 66-67 0.060870165816522305 68-69 0.06337711107813994 70-71 0.058017876054579774 72-73 0.05325979627232588 74-75 0.05629115355295538 76-77 0.06613986708073899 78-79 0.059181814926045115 80-81 0.05745509242442071 82-83 0.06209805737323299 84-85 0.06402942846764251 86-87 0.05769811262835303 88-89 0.056930680405408864 90-91 0.059424835129977434 92-93 0.05354118808740541 94-95 0.06327478678174739 96-97 0.04933310139826152 98-99 0.048962175823838494 100-101 0.046864527747791075 102-103 0.04512501470911761 104-105 0.0438203799301125 106-107 0.055255120051980744 108-109 0.055549302404109345 110-111 0.062085266836183914 112-113 0.06228991542896903 114-115 0.06479686069058668 116-117 0.06766194098957827 118-119 0.06090853742766951 120-121 0.06266084100339205 122-123 0.05997482822308743 124-125 0.07222816271609613 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3909140.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.026432331744644 #Duplication Level Percentage of deduplicated Percentage of total 1 72.05631728987467 36.76776798266511 2 13.196137563763626 13.467036408755567 3 5.5377021305502945 8.477075491136482 4 2.8789588644246624 5.876119987257657 5 1.6814877955522833 4.290016160820152 6 1.05078489731397 3.2170682674806312 7 0.7470107425949967 2.668210517567694 8 0.5267865519557168 2.1504030677313173 9 0.380141382475753 1.7457532676455383 >10 1.838146370669628 16.01805866110528 >50 0.07988661272693916 2.789993484918552 >100 0.025783977161475343 2.1438639513728472 >500 6.041089169488486E-4 0.19471454827133203 >1k 2.517120190067375E-4 0.1939182032718952 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.5581074098139233E-5 0.0 10-11 0.0 0.0 0.0 5.1162148196278466E-5 0.0 12-13 0.0 0.0 0.0 5.1162148196278466E-5 0.0 14-15 0.0 0.0 0.0 7.67432222944177E-5 2.5581074098139233E-5 16-17 0.0 0.0 0.0 7.67432222944177E-5 2.5581074098139233E-5 18-19 0.0 0.0 0.0 1.0232429639255693E-4 5.1162148196278466E-5 20-21 0.0 0.0 0.0 1.534864445888354E-4 5.1162148196278466E-5 22-23 0.0 0.0 0.0 2.174391298341835E-4 5.1162148196278466E-5 24-25 0.0 0.0 0.0 4.34878259668367E-4 5.1162148196278466E-5 26-27 0.0 0.0 0.0 8.825470563858035E-4 5.1162148196278466E-5 28-29 0.0 0.0 0.0 0.0030569383547276383 5.1162148196278466E-5 30-31 0.0 0.0 0.0 0.011601017103506143 6.395268524534808E-5 32-33 0.0 0.0 0.0 0.03416352445806495 7.67432222944177E-5 34-35 0.0 0.0 0.0 0.06964447423218406 7.67432222944177E-5 36-37 0.0 1.2790537049069617E-5 0.0 0.12867280271364034 7.67432222944177E-5 38-39 0.0 2.5581074098139233E-5 0.0 0.22223558122758458 7.67432222944177E-5 40-41 0.0 2.5581074098139233E-5 0.0 0.3287807548463345 7.67432222944177E-5 42-43 0.0 2.5581074098139233E-5 0.0 0.4333050236113314 7.67432222944177E-5 44-45 0.0 2.5581074098139233E-5 0.0 0.559074374414833 7.67432222944177E-5 46-47 0.0 2.5581074098139233E-5 0.0 0.7184444660462403 7.67432222944177E-5 48-49 0.0 2.5581074098139233E-5 0.0 0.8800401111241859 7.67432222944177E-5 50-51 0.0 2.5581074098139233E-5 0.0 1.0515867940263077 7.67432222944177E-5 52-53 0.0 2.5581074098139233E-5 1.2790537049069617E-5 1.2261136720608623 7.67432222944177E-5 54-55 0.0 3.837161114720885E-5 2.5581074098139233E-5 1.4216554024670387 7.67432222944177E-5 56-57 0.0 5.1162148196278466E-5 2.5581074098139233E-5 1.6595721821167828 7.67432222944177E-5 58-59 0.0 7.67432222944177E-5 2.5581074098139233E-5 1.9171352266738975 7.67432222944177E-5 60-61 0.0 7.67432222944177E-5 2.5581074098139233E-5 2.183063282461104 7.67432222944177E-5 62-63 0.0 7.67432222944177E-5 2.5581074098139233E-5 2.449502959730273 7.67432222944177E-5 64-65 0.0 7.67432222944177E-5 2.5581074098139233E-5 2.737750502668106 7.67432222944177E-5 66-67 0.0 7.67432222944177E-5 2.5581074098139233E-5 3.0758939306343596 7.67432222944177E-5 68-69 0.0 7.67432222944177E-5 2.5581074098139233E-5 3.4525368751183123 7.67432222944177E-5 70-71 0.0 7.67432222944177E-5 2.5581074098139233E-5 3.858431777833488 7.67432222944177E-5 72-73 0.0 7.67432222944177E-5 2.5581074098139233E-5 4.26024649923001 7.67432222944177E-5 74-75 0.0 7.67432222944177E-5 2.5581074098139233E-5 4.675811047954282 7.67432222944177E-5 76-77 0.0 7.67432222944177E-5 2.5581074098139233E-5 5.137690131333235 7.67432222944177E-5 78-79 0.0 7.67432222944177E-5 2.5581074098139233E-5 5.657740577211356 7.67432222944177E-5 80-81 0.0 7.67432222944177E-5 2.5581074098139233E-5 6.203858137595481 7.67432222944177E-5 82-83 0.0 7.67432222944177E-5 2.5581074098139233E-5 6.76147439078672 7.67432222944177E-5 84-85 0.0 7.67432222944177E-5 2.5581074098139233E-5 7.3291951682467245 7.67432222944177E-5 86-87 0.0 7.67432222944177E-5 2.5581074098139233E-5 7.926538829512374 7.67432222944177E-5 88-89 0.0 7.67432222944177E-5 2.5581074098139233E-5 8.59072583739646 7.67432222944177E-5 90-91 0.0 8.953375934348732E-5 2.5581074098139233E-5 9.287515924218626 7.67432222944177E-5 92-93 0.0 1.0232429639255693E-4 2.5581074098139233E-5 9.995024481087913 7.67432222944177E-5 94-95 0.0 1.0232429639255693E-4 2.5581074098139233E-5 10.703952787569644 7.67432222944177E-5 96-97 0.0 1.0232429639255693E-4 2.5581074098139233E-5 11.438027289889849 7.67432222944177E-5 98-99 0.0 1.0232429639255693E-4 2.5581074098139233E-5 12.224542994111237 7.67432222944177E-5 100-101 0.0 1.0232429639255693E-4 2.5581074098139233E-5 13.067119622218698 7.67432222944177E-5 102-103 0.0 1.0232429639255693E-4 2.5581074098139233E-5 13.92727044823158 7.67432222944177E-5 104-105 0.0 1.4069590753976578E-4 2.5581074098139233E-5 14.772929595767867 1.0232429639255693E-4 106-107 0.0 1.534864445888354E-4 2.5581074098139233E-5 15.630010692888973 1.0232429639255693E-4 108-109 0.0 1.534864445888354E-4 2.5581074098139233E-5 16.516023984815074 1.0232429639255693E-4 110-111 0.0 1.534864445888354E-4 2.5581074098139233E-5 17.4622295440941 1.0232429639255693E-4 112-113 0.0 1.534864445888354E-4 2.5581074098139233E-5 18.429449955744744 1.0232429639255693E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7460 0.0 34.744915 1 GTACATG 13185 0.0 32.312214 1 TACATGG 13540 0.0 31.011444 2 TCAACGC 8180 0.0 29.925022 4 CAACGCA 8250 0.0 29.743305 5 ACATGGG 13920 0.0 29.009619 3 ATCAACG 8485 0.0 28.709326 3 AACGCAG 8560 0.0 28.508001 6 CATGGGG 10000 0.0 28.34999 4 TATCAAC 8820 0.0 28.091587 2 GGTATCA 5125 0.0 27.903467 1 ACGCAGA 10045 0.0 23.817373 7 CGCAGAG 10490 0.0 22.521608 8 ATGGGGA 5810 0.0 20.50217 5 GTAATAC 800 0.0 20.101353 3 GCAGAGT 11725 0.0 19.28633 9 GTATAAT 1710 0.0 18.816484 1 ATGGGGG 5855 0.0 18.71703 5 AGAGTAC 10025 0.0 18.25147 10-11 GTATAAG 1675 0.0 17.43099 1 >>END_MODULE