FastQCFastQC Report
Mon 15 Aug 2016
SRR2920922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909140
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58330.14921440521444615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47700.12202172344812415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70100.041.1866951
GTATCAA101300.037.157761
TCAACGC122250.030.3927084
ATCAACG122950.030.3649583
CAACGCA123500.030.0847055
TATCAAC126150.030.067092
GTACATG135800.029.0795571
AACGCAG128450.029.0028326
TACATGG139300.027.6989382
CATGGGG94500.026.7157734
ACATGGG142450.026.0828973
ACGCAGA150750.024.6673727
CGCAGAG154300.024.136558
GAGTACT90300.020.95348212-13
GCAGAGT169950.020.9348139
AGAGTAC151800.020.14638110-11
AGTACTT95750.019.60547812-13
TATACCG5001.8189894E-1219.0536465
ATGGGGG54150.018.9128995
CAGAGTA165300.018.73499510-11
Produced by FastQC (version 0.11.5)