FastQCFastQC Report
Mon 15 Aug 2016
SRR2920921_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920921_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457534
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG53200.029.7823374
GTACATG101850.027.5577341
GTATCAA69200.027.5567171
TACATGG101300.026.9898782
ACATGGG98650.026.4468943
AACGCAG73000.025.3591236
TCAACGC75200.024.475384
GGTATCA54200.023.96851
CAACGCA76300.023.966395
TATCAAC78800.023.6600572
ATCAACG77500.023.595783
ACGCAGA81750.022.6429987
CGCAGAG83800.021.6611638
ATGGGGG31400.021.2460025
GTATAGC9850.019.3596461
GCAGAGT91850.019.1139779
GTATAGT6900.018.1365151
ATGGGGA31250.017.9170555
GTGTAGC16000.017.5049761
TACACCG2050.001376290817.4335655
Produced by FastQC (version 0.11.5)