##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920921_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2457534
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.75894982531269	31.0	30.0	34.0	25.0	34.0
2	29.85269623940096	31.0	30.0	34.0	25.0	34.0
3	29.92137891072921	31.0	30.0	34.0	25.0	34.0
4	33.30455977414758	37.0	35.0	37.0	27.0	37.0
5	33.393394760764245	37.0	35.0	37.0	27.0	37.0
6	33.34512971132851	37.0	35.0	37.0	27.0	37.0
7	33.30401573284439	37.0	35.0	37.0	27.0	37.0
8	33.21416550086387	37.0	35.0	37.0	26.0	37.0
9	34.60917244685119	39.0	35.0	39.0	26.0	39.0
10-11	34.63560382074063	39.0	35.0	39.0	27.0	39.0
12-13	34.587117004281524	39.0	35.0	39.0	27.0	39.0
14-15	35.74463364494652	40.0	36.0	41.0	26.5	41.0
16-17	35.737979413509635	40.0	36.0	41.0	26.5	41.0
18-19	35.725617834788856	40.0	36.0	41.0	26.0	41.0
20-21	35.756372648354	40.0	36.0	41.0	26.0	41.0
22-23	35.684910768274214	40.0	36.0	41.0	26.0	41.0
24-25	35.692715746760776	40.0	36.0	41.0	26.0	41.0
26-27	35.63694459568006	40.0	36.0	41.0	26.0	41.0
28-29	35.529685245453365	40.0	35.0	41.0	26.0	41.0
30-31	35.377445642664554	40.0	35.0	41.0	25.0	41.0
32-33	35.244244230191725	40.0	35.0	41.0	25.0	41.0
34-35	35.172742879650905	40.0	35.0	41.0	24.5	41.0
36-37	35.028171736382895	40.0	35.0	41.0	24.0	41.0
38-39	34.89144666971037	39.0	35.0	41.0	24.0	41.0
40-41	34.76305719473261	39.0	34.5	41.0	23.0	41.0
42-43	34.571174600229334	39.0	34.0	41.0	22.0	41.0
44-45	34.41073409360766	39.0	34.0	41.0	21.0	41.0
46-47	34.21514534488638	39.0	33.0	41.0	19.5	41.0
48-49	34.09146668977927	39.0	33.0	41.0	18.5	41.0
50-51	33.89792979466408	38.5	33.0	40.5	17.5	41.0
52-53	33.7174981098939	38.0	33.0	40.0	15.5	41.0
54-55	33.48972506585871	38.0	33.0	40.0	13.5	41.0
56-57	33.22960435135384	38.0	32.5	40.0	11.0	41.0
58-59	32.97566910569701	37.5	32.0	40.0	8.5	41.0
60-61	32.71766494380139	37.0	32.0	40.0	8.0	41.0
62-63	32.40816505488836	37.0	31.0	40.0	7.0	41.0
64-65	32.131197777935114	36.0	31.0	39.5	4.0	41.0
66-67	31.782177784722407	36.0	31.0	39.0	2.0	41.0
68-69	31.457595093292706	35.0	31.0	39.0	2.0	40.0
70-71	31.114184381579257	35.0	30.0	38.0	2.0	40.0
72-73	31.171147174362595	35.0	31.0	38.0	2.0	40.0
74-75	31.1616014264706	35.0	31.0	37.0	2.0	39.5
76-77	30.893873289240354	35.0	31.0	37.0	2.0	39.0
78-79	30.593810502723464	35.0	31.0	36.5	2.0	39.0
80-81	30.31276372982022	35.0	31.0	36.0	2.0	38.0
82-83	30.066026146535513	35.0	31.0	36.0	2.0	37.0
84-85	29.817635076462828	35.0	31.0	35.0	2.0	37.0
86-87	29.613042790048887	35.0	30.5	35.0	2.0	36.5
88-89	29.409201052762647	35.0	30.0	35.0	2.0	36.0
90-91	29.23576540548371	35.0	30.0	35.0	2.0	36.0
92-93	29.060365594128097	34.5	30.0	35.0	2.0	35.5
94-95	28.9424874691459	34.0	30.0	35.0	2.0	35.0
96-97	28.83764436219397	34.0	30.0	35.0	2.0	35.0
98-99	28.705606514497866	34.0	29.0	35.0	2.0	35.0
100-101	28.580755749462675	34.0	29.0	35.0	2.0	35.0
102-103	27.230053785624126	32.5	26.0	34.5	2.0	35.0
104-105	28.233824842301267	34.0	29.0	35.0	2.0	35.0
106-107	28.376303237310246	34.0	29.0	35.0	2.0	35.0
108-109	28.304379308689118	34.0	29.0	35.0	2.0	35.0
110-111	28.198406817565903	34.0	29.0	35.0	2.0	35.0
112-113	28.07965179728948	34.0	29.0	35.0	2.0	35.0
114-115	27.95923515198569	34.0	29.0	35.0	2.0	35.0
116-117	27.742911796947674	34.0	29.0	35.0	2.0	35.0
118-119	27.537472319813276	34.0	28.0	35.0	2.0	35.0
120-121	27.35396519437778	34.0	27.5	35.0	2.0	35.0
122-123	27.060047999335918	34.0	27.0	35.0	2.0	35.0
124-125	25.794680765352584	33.0	22.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94730.0
3	13569.0
4	9250.0
5	8980.0
6	12227.0
7	12321.0
8	11340.0
9	12115.0
10	13144.0
11	14410.0
12	15127.0
13	14772.0
14	14877.0
15	13376.0
16	10798.0
17	9706.0
18	9353.0
19	9450.0
20	9885.0
21	10481.0
22	12451.0
23	16233.0
24	21854.0
25	27883.0
26	30686.0
27	34029.0
28	39121.0
29	46899.0
30	55221.0
31	67276.0
32	83925.0
33	107578.0
34	146008.0
35	210449.0
36	371662.0
37	618229.0
38	247539.0
39	580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.355053227802934	19.084161432331058	12.48728862434283	25.07349671552318
2	15.737839864380257	20.840328943078827	39.15408815283302	24.267743039707895
3	18.77793941953264	25.240461924942696	29.47838808070276	26.5032105748219
4	12.70387906601841	16.86986825821211	36.74279238473867	33.68346029103081
5	13.399444351147901	38.381835760764474	34.57043191589616	13.64828797219147
6	32.149809707193896	37.18325097427554	17.16065128491943	13.50628803361113
7	27.258566534049226	33.27893940807298	21.48948381848614	17.973010239391655
8	25.427000308324054	36.34203849855552	20.1625604989229	18.06840069419752
9	25.906410738928614	16.58288080004301	19.47897166188472	38.03173679914365
10-11	24.52989869164588	26.97614417349692	28.00289161533371	20.491065519523495
12-13	24.8506556004837	24.675019786007734	27.74990893447945	22.72441567902911
14-15	22.446185957272917	26.18341619987006	25.121663034574677	26.248734808282347
16-17	22.56648510810543	28.71687553943783	26.058122765513364	22.65851658694338
18-19	22.579648148355524	28.058117315779775	27.39332314624311	21.96891138962159
20-21	23.442258715954583	27.22114554378301	27.14292432922051	22.193671411041898
22-23	23.055107229174673	27.04020849755379	26.807069731536192	23.09761454173534
24-25	22.751076403090334	27.079490248395704	27.15512195638361	23.014311392130352
26-27	22.617184620878856	27.282233838643588	27.070870079190655	23.029711461286897
28-29	22.6685128811533	27.551330789189503	27.059298747961996	22.720857581695196
30-31	23.053679114292578	27.371482436102596	26.93217122916743	22.6426672204374
32-33	22.613495458552805	27.41486695320915	26.892799042234135	23.07883854600391
34-35	22.802895139063224	27.510336364763578	27.030548241153546	22.656220255019655
36-37	22.949226862477133	27.26551772442	26.97405391846262	22.811201494640244
38-39	22.73814398063042	27.478678619005258	26.991120443177696	22.79205695718662
40-41	22.840436606839447	27.4508945578072	27.04742680377023	22.66124203158312
42-43	22.730862710436732	27.456752086424363	26.890558340576696	22.921826862562213
44-45	22.825026716672163	27.400097766019975	26.948321842806006	22.82655367450186
46-47	22.65435359960748	27.43301665053223	26.904417984651417	23.008211765208866
48-49	22.551526349261152	27.50307191580874	26.99519812126596	22.95020361366414
50-51	22.507391692842717	27.557460576111275	26.937093808079222	22.998053922966783
52-53	22.662661937160657	27.48957166726488	26.729513204674642	23.11825319089982
54-55	22.611860547831807	27.33236518130048	26.777085946532843	23.27868832433487
56-57	22.575773215921423	27.504129512254366	26.66413795757266	23.255959314251555
58-59	22.62073267230388	27.23685766790161	26.91525266390852	23.227156995885988
60-61	22.7087920119778	27.235749184507203	26.943877889650008	23.111580913864984
62-63	22.545246151277887	27.233297095695864	27.037003106664876	23.184453646361376
64-65	22.563481516586133	27.229364842835203	26.88966625789651	23.31748738268215
66-67	22.567861684860226	27.478890337243623	26.67328571338415	23.279962264512008
68-69	22.809987752283416	27.16688491055258	26.79961628164536	23.22351105551864
70-71	22.54722254907509	27.11140055120287	26.950412506417738	23.390964393304298
72-73	22.68982386599126	27.193410303241027	26.86752045234138	23.24924537842633
74-75	22.641372694952878	27.284893482923557	26.7548120377895	23.31892178433407
76-77	22.599768393346928	27.06493537164561	26.96358710169193	23.371709133315527
78-79	22.717274269098827	27.107842009592193	26.88856809512632	23.28631562618266
80-81	22.71000124791412	27.124502539291484	26.92923349776078	23.23626271503362
82-83	22.66273126555122	27.092215503520567	27.024524052757393	23.22052917817082
84-85	22.744706273479974	27.10601337690372	26.745521436821072	23.403758912795233
86-87	22.6251754558289	27.100395850743197	26.89944739684113	23.374981296586768
88-89	22.74160057326189	26.82977623243164	26.954607266745924	23.474015927560544
90-91	22.82993527992334	26.927855569039327	26.85012877444714	23.392080376590187
92-93	22.951117683210214	26.85888156562166	26.886078330346642	23.303922420821486
94-95	22.74632737432738	26.928109392015266	26.887494149471014	23.43806908418634
96-97	22.68854107507445	26.87720733869507	26.904504548517178	23.529747037713303
98-99	22.601147503408598	26.943796108682648	26.796846787979756	23.658209599929
100-101	22.575339643891702	27.192640165252683	26.73317973203841	23.498840458817206
102-103	22.598773571578906	27.18186077924573	26.751210409049662	23.468155240125697
104-105	22.51712285199778	27.282027503628726	26.614414887889566	23.586434756483925
106-107	22.407551262696586	27.44072890660752	26.65937076067963	23.492349070016267
108-109	22.451862779684646	27.44267880854896	26.629648501138863	23.475809910627532
110-111	22.417600449184665	27.748003821652677	26.455661438868926	23.378734290293732
112-113	22.432906226594515	27.900956893089084	26.374158369541963	23.291978510774438
114-115	22.508478951698425	27.95219090737151	26.294797357177075	23.244532783752987
116-117	22.418768910215757	27.918741730200374	26.317278936383904	23.345210423199966
118-119	22.51919423015731	27.872345060273535	26.24069553420251	23.367765175366646
120-121	22.54809669420478	28.0802446264032	26.235224899124184	23.136433780267833
122-123	22.628431924010965	28.095874718576276	26.057836634075564	23.217856723337196
124-125	22.747319220032345	28.220168146956848	25.830529221300786	23.20198341171002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2152.0
1	1564.0
2	805.5
3	562.5
4	446.5
5	391.0
6	381.5
7	402.0
8	458.0
9	532.5
10	562.5
11	580.0
12	660.5
13	738.0
14	814.5
15	978.5
16	1138.5
17	1312.5
18	1570.0
19	1909.0
20	2439.0
21	3224.5
22	4109.5
23	5167.5
24	6543.5
25	8501.0
26	11306.0
27	14751.0
28	18012.5
29	21570.5
30	25832.0
31	30449.0
32	35767.0
33	40380.5
34	44741.0
35	49575.0
36	55509.5
37	60787.5
38	64551.0
39	68573.0
40	72951.5
41	77305.5
42	80213.5
43	83381.0
44	87036.0
45	90386.0
46	93368.5
47	95037.5
48	96509.5
49	98518.5
50	100008.5
51	98034.5
52	92673.5
53	88030.5
54	84081.0
55	78829.0
56	71936.5
57	64809.0
58	58875.5
59	51724.5
60	43506.0
61	36468.0
62	29903.0
63	22858.0
64	16731.0
65	12875.5
66	10094.0
67	7756.5
68	5831.5
69	4390.5
70	3418.5
71	2618.0
72	1992.0
73	1649.0
74	1372.0
75	1028.5
76	698.5
77	482.5
78	356.0
79	273.0
80	205.0
81	131.5
82	91.5
83	72.0
84	55.0
85	43.0
86	37.5
87	30.0
88	21.5
89	16.0
90	16.0
91	19.5
92	14.0
93	10.5
94	9.5
95	8.5
96	11.5
97	10.5
98	11.0
99	13.0
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.163700685321139
2	0.1470579857694746
3	0.17232721907407994
4	0.14514549951292638
5	0.17033335042363607
6	0.10713992156364877
7	0.10591918565521373
8	0.09460703290371567
9	0.10034449167336038
10-11	0.08785229421037512
12-13	0.0766825606481945
14-15	0.07643841346650748
16-17	0.07538044234586377
18-19	0.07371210327100255
20-21	0.06830017407694054
22-23	0.06046711866448236
24-25	0.0632137744584612
26-27	0.07385452246031998
28-29	0.06909365241742332
30-31	0.06689632778224024
32-33	0.07401728724811131
34-35	0.07171823462055865
36-37	0.07430212562674617
38-39	0.07017196913654095
40-41	0.07420039763437658
42-43	0.07477007439164626
44-45	0.06797464450135787
46-47	0.06537040789669644
48-49	0.0574559700903426
50-51	0.0637834512157309
52-53	0.06528902550280077
54-55	0.06724220295629683
56-57	0.07061957230296713
58-59	0.05759838927966002
60-61	0.0674253133425621
62-63	0.062013384148500085
64-65	0.05639799896969889
66-67	0.06065022905074762
68-69	0.06372241442030914
70-71	0.059042926771308145
72-73	0.054363439122307164
74-75	0.05704905812086425
76-77	0.06697771017613592
78-79	0.059958478702634425
80-81	0.05841221321861671
82-83	0.06254236970882193
84-85	0.06447520156384408
86-87	0.05824944843082537
88-89	0.05753735248423827
90-91	0.06085368503548679
92-93	0.05531968225058127
94-95	0.06345792164014821
96-97	0.050416393018367194
98-99	0.050660540200054206
100-101	0.04779181081523186
102-103	0.04610312614189671
104-105	0.04453651505940508
106-107	0.05542141024295086
108-109	0.055034843871946425
110-111	0.062196494534765334
112-113	0.06154543538359999
114-115	0.0650855695180616
116-117	0.06858501245557538
118-119	0.061952347353078335
120-121	0.06282720808745677
122-123	0.06085368503548679
124-125	0.07184030821140217
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2457534.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.55263504638642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.71549670551825	37.87001187194561
2	14.467860460081965	15.495841022416279
3	6.044620500083946	9.711160669047041
4	3.0244107560123497	6.4786066178838
5	1.7524431102915892	4.6923973162499895
6	1.0920773564608368	3.509017206778179
7	0.6775506102791388	2.5399234390414582
8	0.4973418427347185	2.1307172957815754
9	0.34463323428898773	1.6610416038660554
>10	1.306566227421418	11.291352017482827
>50	0.05332660218339369	1.9156959148433756
>100	0.022453857414011274	2.038148681430618
>500	8.378818452362319E-4	0.2974116926036872
>1k	3.8085538419603294E-4	0.3686746506294688
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0691196947834696E-5
2	0.0	0.0	0.0	4.0691196947834696E-5	4.0691196947834696E-5
3	0.0	0.0	0.0	4.0691196947834696E-5	4.0691196947834696E-5
4	0.0	0.0	0.0	4.0691196947834696E-5	4.0691196947834696E-5
5	0.0	0.0	0.0	4.0691196947834696E-5	4.0691196947834696E-5
6	0.0	0.0	0.0	4.0691196947834696E-5	1.220735908435041E-4
7	0.0	0.0	0.0	4.0691196947834696E-5	1.220735908435041E-4
8	0.0	0.0	0.0	4.0691196947834696E-5	1.220735908435041E-4
9	0.0	0.0	0.0	8.138239389566939E-5	1.220735908435041E-4
10-11	0.0	0.0	0.0	1.220735908435041E-4	1.220735908435041E-4
12-13	0.0	0.0	0.0	1.220735908435041E-4	2.034559847391735E-4
14-15	0.0	0.0	0.0	1.220735908435041E-4	2.441471816870082E-4
16-17	0.0	0.0	0.0	1.627647877913388E-4	3.051839771087602E-4
18-19	0.0	0.0	0.0	2.441471816870082E-4	3.2552957558267757E-4
20-21	0.0	0.0	0.0	3.865663710044296E-4	3.6622077253051227E-4
22-23	0.0	0.0	0.0	6.103679542175204E-4	3.6622077253051227E-4
24-25	0.0	0.0	0.0	9.562431282741155E-4	3.865663710044296E-4
26-27	0.0	0.0	0.0	0.0017497214687568921	4.882943633740164E-4
28-29	0.0	0.0	0.0	0.004170847687153057	4.882943633740164E-4
30-31	0.0	0.0	0.0	0.01597129480202512	4.882943633740164E-4
32-33	0.0	0.0	0.0	0.04722213405796217	4.882943633740164E-4
34-35	0.0	0.0	0.0	0.09798440225038596	5.289855603218511E-4
36-37	0.0	0.0	0.0	0.18343591584083885	5.289855603218511E-4
38-39	0.0	0.0	0.0	0.3194258960405024	6.103679542175204E-4
40-41	0.0	0.0	0.0	0.47289274532926096	6.510591511653551E-4
42-43	0.0	0.0	0.0	0.6144777651092518	6.714047496392725E-4
44-45	0.0	0.0	0.0	0.7894092207879932	7.324415450610245E-4
46-47	0.0	0.0	0.0	1.0002710033716726	7.731327420088592E-4
48-49	0.0	0.0	0.0	1.2156698544150355	7.731327420088592E-4
50-51	0.0	0.0	0.0	1.4426453509900576	8.13823938956694E-4
52-53	0.0	0.0	0.0	1.6665486621955181	8.545151359045287E-4
54-55	0.0	0.0	0.0	1.9174709281743407	8.545151359045287E-4
56-57	0.0	0.0	0.0	2.221393478177718	8.545151359045287E-4
58-59	0.0	0.0	0.0	2.545763354647382	8.545151359045287E-4
60-61	0.0	0.0	0.0	2.8737954388423517	8.545151359045287E-4
62-63	0.0	0.0	0.0	3.20815500416271	9.358975298001981E-4
64-65	0.0	0.0	0.0	3.5630025871463022	9.765887267480329E-4
66-67	0.0	0.0	0.0	3.982508482080004	9.969343252219502E-4
68-69	0.0	0.0	0.0	4.443254905120336	0.0010172799236958675
70-71	0.0	0.0	0.0	4.932546202819575	0.0010376255221697848
72-73	0.0	0.0	0.0	5.417870108816398	0.0010579711206437023
74-75	0.0	0.0	0.0	5.9084838704164415	0.0010783167191176196
76-77	0.0	0.0	0.0	6.459340949097754	0.001342809499278545
78-79	0.0	0.0	0.0	7.069342682542744	0.0013631550977524624
80-81	0.0	0.0	0.0	7.712365322310902	0.0013835006962263797
82-83	0.0	0.0	0.0	8.368287071511524	0.001505574287069884
84-85	0.0	0.0	0.0	9.021848731289172	0.001505574287069884
86-87	0.0	0.0	0.0	9.718868589407105	0.001586956680965553
88-89	0.0	0.0	0.0	10.478471508430808	0.001627647877913388
90-91	0.0	0.0	0.0	11.279152190773353	0.001627647877913388
92-93	0.0	0.0	0.0	12.094013755252217	0.0017700670672308094
94-95	4.0691196947834696E-5	0.0	0.0	12.90830564297381	0.0018311038626525615
96-97	4.0691196947834696E-5	0.0	0.0	13.750857566975675	0.0019328318550221482
98-99	4.0691196947834696E-5	0.0	0.0	14.645656987858562	0.0020549054458656522
100-101	4.0691196947834696E-5	0.0	0.0	15.613212268884174	0.0020752510443395695
102-103	4.0691196947834696E-5	0.0	0.0	16.583534551302243	0.002115942241287404
104-105	4.0691196947834696E-5	0.0	0.0	17.55052015557058	0.002156633438235239
106-107	4.0691196947834696E-5	0.0	0.0	18.521493497139815	0.002156633438235239
108-109	4.0691196947834696E-5	0.0	0.0	19.534399117163794	0.0022583614306048256
110-111	4.0691196947834696E-5	0.0	0.0	20.59411995927625	0.002319398226026578
112-113	4.0691196947834696E-5	0.0	0.0	21.671968729628972	0.002319398226026578
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	5320	0.0	29.782337	4
GTACATG	10185	0.0	27.557734	1
GTATCAA	6920	0.0	27.556717	1
TACATGG	10130	0.0	26.989878	2
ACATGGG	9865	0.0	26.446894	3
AACGCAG	7300	0.0	25.359123	6
TCAACGC	7520	0.0	24.47538	4
GGTATCA	5420	0.0	23.9685	1
CAACGCA	7630	0.0	23.96639	5
TATCAAC	7880	0.0	23.660057	2
ATCAACG	7750	0.0	23.59578	3
ACGCAGA	8175	0.0	22.642998	7
CGCAGAG	8380	0.0	21.661163	8
ATGGGGG	3140	0.0	21.246002	5
GTATAGC	985	0.0	19.359646	1
GCAGAGT	9185	0.0	19.113977	9
GTATAGT	690	0.0	18.136515	1
ATGGGGA	3125	0.0	17.917055	5
GTGTAGC	1600	0.0	17.504976	1
TACACCG	205	0.0013762908	17.433565	5
>>END_MODULE