FastQCFastQC Report
Mon 15 Aug 2016
SRR2920921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457534
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60650.24679210948861746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50100.20386289670865182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36100.14689522098168326No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT24780.10083278603673439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59050.040.916511
GTATCAA86700.040.1843721
ATCAACG109150.031.639933
TATCAAC109950.031.6276232
TCAACGC108900.031.5485324
CAACGCA109550.031.4156995
AACGCAG111100.031.0133136
ACGCAGA122900.027.8852627
CGCAGAG126700.027.0936788
CATGGGG54150.026.0604024
GCAGAGT137050.024.615479
GTACATG97700.024.0583081
GAGTACT84000.023.76436212-13
AGTACTT90100.023.6743112-13
TACATGG96500.023.6330832
ACATGGG94000.023.6271973
AGAGTAC129600.022.31229410-11
GTACTTT92750.021.61866814-15
CAGAGTA134900.021.14898910-11
GTGTACG3602.1118467E-919.885761
Produced by FastQC (version 0.11.5)