FastQCFastQC Report
Mon 15 Aug 2016
SRR2920920_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920920_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654826
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103700.033.1519171
GTACATG166450.029.3522641
TACATGG166050.028.83742
TCAACGC119350.027.9434934
CAACGCA121250.027.6529675
GGTATCA75400.027.578181
AACGCAG120900.027.5212766
CATGGGG113750.027.172594
ATCAACG123200.027.1684283
ACATGGG171450.027.1649043
TATCAAC125700.027.1018942
ACGCAGA134300.024.6396337
CGCAGAG137750.023.6742828
ATGGGGA58800.020.965645
GCAGAGT152100.020.7361729
AGAGTAC133500.018.71946710-11
CTATACT9150.018.224354
CAGAGTA138850.017.8696310-11
ATGGGGG53500.017.6993965
GTATAGG6400.017.6882651
Produced by FastQC (version 0.11.5)