FastQCFastQC Report
Mon 15 Aug 2016
SRR2920920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654826
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96390.26373348553392145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78400.2145108960043515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55020.1505406823744824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93200.044.6719171
GTATCAA146350.043.120921
TATCAAC185000.034.1820342
TCAACGC185200.033.82364
ATCAACG185400.033.819233
CAACGCA187350.033.4032065
AACGCAG193250.032.458056
ACGCAGA217200.028.5439537
CGCAGAG223450.027.689668
GCAGAGT237550.025.4453989
GTACATG159650.025.4432891
GAGTACT131050.024.85760312-13
TACATGG162150.024.6772862
CATGGGG106900.024.5643884
AGTACTT137150.024.40275812-13
GTATAGG5700.024.0684451
ACATGGG163300.023.7742313
AGAGTAC218250.022.77678910-11
GTACTTT144700.022.63606614-15
CAGAGTA222250.022.42039710-11
Produced by FastQC (version 0.11.5)