FastQCFastQC Report
Mon 15 Aug 2016
SRR2920919_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920919_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888320
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40370.1397698315976069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91700.031.5768891
CATGGGG75550.030.8206144
GTACATG132900.028.9159281
TACATGG131400.028.598322
AACGCAG99950.028.3453776
TCAACGC104250.027.0199184
CAACGCA105100.026.7447345
ACATGGG136700.026.6617983
ATCAACG106450.026.461963
TATCAAC108750.026.4499232
ACGCAGA112450.024.9801737
ATGGGGA43050.024.8999425
GGTATCA69300.024.8466011
CGCAGAG113500.024.43328
GCAGAGT124400.021.0474769
GAGTACT38200.020.9487912-13
ATGGGGG41800.019.9457665
CTATACT6950.018.8511074
GTACTTT43600.018.21775214-15
CGTACAC3604.6607965E-818.196873
Produced by FastQC (version 0.11.5)