##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920919_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2888320 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.46842870596056 31.0 30.0 34.0 23.0 34.0 2 29.551229780633726 31.0 30.0 34.0 23.0 34.0 3 29.61923436461334 31.0 30.0 34.0 23.0 34.0 4 32.97910169233326 37.0 35.0 37.0 25.0 37.0 5 33.080815837580325 37.0 35.0 37.0 26.0 37.0 6 33.01902905495236 37.0 35.0 37.0 26.0 37.0 7 32.9843078329271 37.0 35.0 37.0 25.0 37.0 8 32.885244017283405 37.0 35.0 37.0 25.0 37.0 9 34.23341492632395 39.0 35.0 39.0 24.0 39.0 10-11 34.26075175188345 39.0 35.0 39.0 25.0 39.0 12-13 34.21136075642588 39.0 35.0 39.0 25.0 39.0 14-15 35.342025814314205 40.0 36.0 41.0 25.0 41.0 16-17 35.33946463688234 40.0 36.0 41.0 25.0 41.0 18-19 35.33388613450033 40.0 35.5 41.0 25.0 41.0 20-21 35.36703793208508 40.0 35.0 41.0 24.5 41.0 22-23 35.292389693662756 40.0 35.0 41.0 24.5 41.0 24-25 35.306080004985596 40.0 35.0 41.0 25.0 41.0 26-27 35.233575919565695 40.0 35.0 41.0 25.0 41.0 28-29 35.12083096748283 40.0 35.0 41.0 24.0 41.0 30-31 34.95727914497009 39.5 34.5 41.0 24.0 41.0 32-33 34.81131263156437 39.0 34.0 41.0 23.0 41.0 34-35 34.73004220446488 39.0 34.0 41.0 23.0 41.0 36-37 34.57704374861511 39.0 34.0 41.0 21.5 41.0 38-39 34.427641154719694 39.0 34.0 41.0 19.5 41.0 40-41 34.2850726373809 39.0 34.0 41.0 18.5 41.0 42-43 34.077948772989146 39.0 33.0 41.0 17.0 41.0 44-45 33.90559287059605 39.0 33.0 41.0 15.0 41.0 46-47 33.68690640233769 38.0 33.0 41.0 13.5 41.0 48-49 33.54592081209839 38.0 33.0 40.0 10.0 41.0 50-51 33.34135535536228 38.0 33.0 40.0 8.5 41.0 52-53 33.13681967372037 38.0 32.0 40.0 7.5 41.0 54-55 32.89884084865943 38.0 32.0 40.0 4.5 41.0 56-57 32.6310498836694 37.5 31.5 40.0 2.0 41.0 58-59 32.36400191114558 37.0 31.0 40.0 2.0 41.0 60-61 32.08837975016618 37.0 31.0 40.0 2.0 41.0 62-63 31.774741545258145 36.0 31.0 40.0 2.0 41.0 64-65 31.485420417405273 36.0 30.5 39.0 2.0 41.0 66-67 31.14353361123421 35.0 30.0 39.0 2.0 40.5 68-69 30.816455240416573 35.0 30.0 38.5 2.0 40.0 70-71 30.47366514098161 35.0 30.0 38.0 2.0 40.0 72-73 30.52778293263904 35.0 30.0 37.0 2.0 40.0 74-75 30.521376613394636 35.0 30.0 37.0 2.0 39.0 76-77 30.25444531769333 35.0 30.0 37.0 2.0 39.0 78-79 29.949326251938842 35.0 30.0 36.0 2.0 39.0 80-81 29.67439705434301 35.0 30.0 36.0 2.0 37.5 82-83 29.423763468036782 35.0 30.0 35.0 2.0 37.0 84-85 29.172266057777534 35.0 30.0 35.0 2.0 37.0 86-87 28.95804758475515 35.0 29.5 35.0 2.0 36.0 88-89 28.744917287558167 34.0 29.0 35.0 2.0 36.0 90-91 28.554863034566807 34.0 29.0 35.0 2.0 36.0 92-93 28.373882049080436 34.0 29.0 35.0 2.0 35.0 94-95 28.2414268502105 34.0 29.0 35.0 2.0 35.0 96-97 28.127335094449368 34.0 29.0 35.0 2.0 35.0 98-99 27.973943330378905 34.0 29.0 35.0 2.0 35.0 100-101 27.821237085918458 34.0 28.5 35.0 2.0 35.0 102-103 26.457894900842014 32.5 24.5 34.0 2.0 35.0 104-105 27.43979631758254 34.0 27.0 35.0 2.0 35.0 106-107 27.56534248282739 34.0 27.0 35.0 2.0 35.0 108-109 27.4754331237536 34.0 27.0 35.0 2.0 35.0 110-111 27.34865008032351 34.0 27.0 35.0 2.0 35.0 112-113 27.19964391064702 34.0 27.0 35.0 2.0 35.0 114-115 27.052983222080655 34.0 25.5 35.0 2.0 35.0 116-117 26.80811994515843 34.0 25.0 35.0 2.0 35.0 118-119 26.575984136106804 34.0 25.0 35.0 2.0 35.0 120-121 26.360780488311544 34.0 24.5 35.0 2.0 35.0 122-123 26.03467171227565 34.0 23.5 35.0 2.0 35.0 124-125 24.740926559384 32.5 10.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 125192.0 3 17289.0 4 12130.0 5 12571.0 6 17281.0 7 17047.0 8 15566.0 9 16398.0 10 17700.0 11 18295.0 12 18589.0 13 18305.0 14 19231.0 15 17841.0 16 14501.0 17 13385.0 18 12814.0 19 12649.0 20 12982.0 21 14201.0 22 16538.0 23 21030.0 24 28477.0 25 35839.0 26 39900.0 27 42391.0 28 49528.0 29 57774.0 30 68252.0 31 82198.0 32 102301.0 33 130681.0 34 175390.0 35 252457.0 36 436557.0 37 677932.0 38 246756.0 39 352.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.12095377536661 18.769190328525244 12.268736384273346 24.8411195118348 2 15.870129780070801 21.09888319100173 38.68756739514096 24.343419633786507 3 18.660571006841664 25.75735347925322 29.414125103221483 26.167950410683638 4 12.913584904090325 17.16156318000452 36.01175658039812 33.91309533550704 5 13.430809834121169 38.65677335673139 34.077539596938365 13.83487721220907 6 31.81577924897987 37.12548554348116 17.096017598969073 13.962717608569896 7 27.140945421978095 33.85740859674291 21.208758187015224 17.792887794263777 8 25.714596623721558 36.09659045226827 19.897010754297977 18.29180216971219 9 25.892301379244376 16.97538737095144 19.309178650200945 37.82313259960324 10-11 24.638216776595122 27.28095267055371 27.575937614626124 20.50489293822504 12-13 24.89656374379478 25.100629678279546 27.471806885263284 22.530999692662395 14-15 22.50166661815803 26.369606705833924 24.911558360740713 26.21716831526733 16-17 22.512089815103874 28.899396314828245 25.90080686544193 22.687707004625953 18-19 22.620049525962322 28.09203776779066 27.300710320241787 21.987202386005226 20-21 23.663314830588412 27.24048908500886 26.925157687352634 22.171038397050093 22-23 23.261894624671367 27.0090837694612 26.74493006768917 22.984091538178262 24-25 22.83170569725172 27.26663149138904 26.89160944910041 23.01005336225882 26-27 22.742317784170698 27.293109678921507 27.03624757854589 22.928324958361905 28-29 22.905469430744738 27.48594661358861 26.981306614142948 22.62727734152371 30-31 23.036321353248653 27.416346557764683 26.70720753883531 22.84012455015136 32-33 22.688656356126806 27.566069635210066 26.74450533506598 23.000768673597143 34-35 22.877511094541937 27.59870924443818 26.824633564391302 22.699146096628578 36-37 22.99367057358099 27.34634444657148 26.799434188950745 22.860550790896784 38-39 22.917963754641367 27.512989935463484 26.794294926363516 22.774751383531633 40-41 22.884289455580372 27.4109243576646 26.955331337464706 22.74945484929032 42-43 22.822992319470128 27.432297724737875 26.79554517486918 22.94916478092282 44-45 22.76383921278756 27.44408006481573 26.79363615434306 22.998444568053657 46-47 22.771243872551143 27.450918913787675 26.779857379286355 22.997979834374828 48-49 22.65294100224117 27.51057840019635 26.657239394795475 23.179241202767003 50-51 22.41562059420992 27.592650750232593 26.743759638714604 23.24796901684288 52-53 22.716660291961496 27.42836425065341 26.47742453596304 23.377550921422056 54-55 22.59535166686992 27.353546048182352 26.494733173316774 23.55636911163095 56-57 22.544795056294383 27.41634837658198 26.490980010089192 23.547876557034442 58-59 22.579353332544642 27.199497822807594 26.54983032986037 23.671318514787394 60-61 22.548412623071343 27.04092804228478 26.753591233724162 23.657068100919712 62-63 22.456925932437827 27.085202337471344 26.724569345935883 23.733302384154946 64-65 22.38046911821178 27.017002707483833 26.668083544522613 23.93444462978177 66-67 22.440924150034938 27.10255426899211 26.562817750626106 23.893703830346848 68-69 22.526998917687425 26.83452674115823 26.660365879853604 23.978108461300742 70-71 22.331088454463256 26.71355622763349 26.74272579566752 24.212629522235734 72-73 22.38448747704992 26.61551252295008 26.77683860463505 24.22316139536495 74-75 22.15560606089475 26.74104704716114 26.856528788938277 24.246818103005836 76-77 22.390620947997693 26.589710129336662 26.963648499318072 24.056020423347572 78-79 22.388659766849248 26.57363940396359 26.92240069856191 24.115300130625254 80-81 22.295566179920918 26.630964318495614 26.921120236378947 24.152349265204517 82-83 22.30059688115436 26.384305405258868 27.044638852862914 24.270458860723863 84-85 22.351947252622377 26.42718176500068 26.886167931494377 24.334703050882574 86-87 22.22790948555845 26.32003691582423 27.084127962385768 24.36792563623155 88-89 22.37686798676781 26.148298498197015 27.011027171440972 24.463806343594204 90-91 22.309688599296173 26.185395076261752 26.968175364956803 24.53674095948527 92-93 22.381549374230953 26.081862674455987 27.002104141493643 24.53448380981942 94-95 22.2653384310585 26.053536507857316 27.082349187244738 24.598775873839443 96-97 22.221183002593026 26.018145121132306 27.023555471725174 24.737116404549493 98-99 22.188360982637583 26.108141541020686 26.90433965992923 24.7991578164125 100-101 22.056693686918635 26.167805221405914 27.022543972714868 24.752957118960584 102-103 21.799208775861047 26.307042213515512 27.01418016923925 24.879568841384188 104-105 21.72337295912509 26.406350749134084 26.906962723675527 24.963313568065303 106-107 21.614491359389433 26.527018265760027 26.946330678623397 24.91215969622714 108-109 21.547427853237146 26.600351414944 26.95810596213698 24.89411476968187 110-111 21.538144936363178 26.848722733726937 26.756688262634125 24.85644406727576 112-113 21.550799925097692 27.024332682644093 26.634456134601038 24.790411257657176 114-115 21.506523382925998 27.073420646682354 26.612821221283994 24.80723474910765 116-117 21.30898791246389 26.99306623242197 26.74086938444948 24.957076470664664 118-119 21.416387588216534 26.963889185775734 26.59719110203263 25.0225321239751 120-121 21.412816457094078 27.11386418435066 26.51152018411923 24.96179917443603 122-123 21.332246657041324 27.105667721296978 26.46105850469993 25.101027116961767 124-125 21.450512563270248 27.160976552870164 26.27582977421507 25.11268110964452 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3292.0 1 2320.0 2 1094.5 3 793.5 4 638.0 5 509.0 6 506.0 7 570.5 8 646.0 9 707.5 10 730.5 11 722.0 12 806.5 13 946.5 14 1048.5 15 1182.5 16 1371.5 17 1604.0 18 1879.5 19 2207.0 20 2728.5 21 3517.5 22 4606.5 23 5909.5 24 7599.5 25 9791.0 26 12662.5 27 16479.0 28 20474.5 29 24859.0 30 29287.5 31 34409.5 32 40311.0 33 44577.5 34 48065.5 35 52472.0 36 58623.5 37 64779.0 38 70894.0 39 77578.5 40 82706.0 41 86989.5 42 91694.5 43 96836.0 44 102054.0 45 107346.5 46 111682.5 47 113298.5 48 114695.0 49 116284.0 50 118232.5 51 117337.0 52 111330.5 53 106887.0 54 102810.5 55 96606.0 56 89777.0 57 82356.5 58 75302.0 59 67080.5 60 55363.0 61 43895.0 62 34658.0 63 26436.0 64 19705.0 65 15119.0 66 12144.0 67 9532.0 68 7227.0 69 5350.0 70 4044.0 71 3140.5 72 2415.0 73 1731.5 74 1207.0 75 900.5 76 670.0 77 458.5 78 346.5 79 261.0 80 158.5 81 119.5 82 99.0 83 70.0 84 53.5 85 52.5 86 51.5 87 39.0 88 27.0 89 20.0 90 13.0 91 12.0 92 13.5 93 10.0 94 12.0 95 14.0 96 11.5 97 11.0 98 10.5 99 17.5 100 26.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16293208508752494 2 0.14575947263461114 3 0.17162225792155994 4 0.14361289607799688 5 0.16930257035231552 6 0.10909456015953913 7 0.10770967205849767 8 0.09611123421227566 9 0.10289718590737867 10-11 0.0893079714159096 12-13 0.07765760026589852 14-15 0.07741524484821626 16-17 0.07661893419011744 18-19 0.07468009084865943 20-21 0.0689501163306005 22-23 0.06034649900288056 24-25 0.06311627520496343 26-27 0.07412613560824285 28-29 0.06941751606470196 30-31 0.06690740638156437 32-33 0.07545909040549524 34-35 0.0728278030135165 36-37 0.07608229005096388 38-39 0.07087164857079549 40-41 0.07587455683580767 42-43 0.07585724573454465 44-45 0.0686731387103922 46-47 0.06657849545756703 48-49 0.05745554509195657 50-51 0.06399914136937736 52-53 0.06619765122978064 54-55 0.06758253933082206 56-57 0.07146022601373808 58-59 0.058494211167737654 60-61 0.06875969421670729 62-63 0.06465696321737203 64-65 0.05856345557278972 66-67 0.06297778639485929 68-69 0.06605916241967649 70-71 0.06058885442056282 72-73 0.056087968092178156 74-75 0.05942901063594061 76-77 0.06978104919122535 78-79 0.06296047529359627 80-81 0.061229365167294485 82-83 0.06576487369820518 84-85 0.0678075836472413 86-87 0.06069272102814093 88-89 0.05965405495235985 90-91 0.06280467538222911 92-93 0.056503434522490584 94-95 0.06697665078661644 96-97 0.05326625858630623 98-99 0.05326625858630623 100-101 0.050998504320850874 102-103 0.04850570573897629 104-105 0.046861151118989586 106-107 0.058494211167737654 108-109 0.058996233104365164 110-111 0.06583411810325726 112-113 0.06555714048304898 114-115 0.0682922944826058 116-117 0.07210073676046976 118-119 0.06528016286284069 120-121 0.06680353977398626 122-123 0.06379140815422114 124-125 0.07577069022822956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2888320.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.37203559781466 #Duplication Level Percentage of deduplicated Percentage of total 1 69.74728581665964 33.738181923744136 2 13.588623270029988 13.146187370863663 3 6.150508329324109 8.92537823552164 4 3.2482092031218346 6.284899648102342 5 1.9405543220405714 4.69342813726198 6 1.2794683860187335 3.713429418886599 7 0.8744364969960693 2.960879135250555 8 0.6186646462542699 2.3940854633376767 9 0.4549827466056178 1.9807597453678587 >10 1.981560430699129 16.0485341117563 >50 0.08398170121252857 2.7383649123616216 >100 0.030217675871351456 2.539448603807617 >500 0.0010046501108795713 0.3053278258954643 >1k 5.023250553041683E-4 0.5310954678425229 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4037 0.1397698315976069 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.7311101263017947E-5 0.0 20-21 0.0 0.0 0.0 1.557999113671615E-4 0.0 22-23 0.0 0.0 0.0 3.11599822734323E-4 0.0 24-25 0.0 0.0 0.0 0.0012637103922003103 1.7311101263017947E-5 26-27 0.0 0.0 0.0 0.0029082650121870154 6.924440505207179E-5 28-29 0.0 1.7311101263017947E-5 0.0 0.0074610846443607355 6.924440505207179E-5 30-31 0.0 3.4622202526035894E-5 0.0 0.025533874362951473 6.924440505207179E-5 32-33 0.0 3.4622202526035894E-5 0.0 0.06820573897629072 6.924440505207179E-5 34-35 0.0 3.4622202526035894E-5 0.0 0.13584021161090185 6.924440505207179E-5 36-37 0.0 3.4622202526035894E-5 0.0 0.24824119211167736 6.924440505207179E-5 38-39 0.0 3.4622202526035894E-5 0.0 0.4176822512740971 6.924440505207179E-5 40-41 0.0 3.4622202526035894E-5 0.0 0.6112203633946377 6.924440505207179E-5 42-43 0.0 3.4622202526035894E-5 0.0 0.7909095945047641 6.924440505207179E-5 44-45 0.0 3.4622202526035894E-5 0.0 1.005913472191447 6.924440505207179E-5 46-47 0.0 3.4622202526035894E-5 0.0 1.2629833259472636 6.924440505207179E-5 48-49 0.0 3.4622202526035894E-5 0.0 1.5332615499667628 6.924440505207179E-5 50-51 1.7311101263017947E-5 3.4622202526035894E-5 0.0 1.8112778362508308 6.924440505207179E-5 52-53 6.924440505207179E-5 3.4622202526035894E-5 0.0 2.0869744349656547 6.924440505207179E-5 54-55 6.924440505207179E-5 3.4622202526035894E-5 0.0 2.38619682029692 6.924440505207179E-5 56-57 6.924440505207179E-5 3.4622202526035894E-5 0.0 2.7428920618214048 6.924440505207179E-5 58-59 6.924440505207179E-5 3.4622202526035894E-5 0.0 3.1275793540881898 6.924440505207179E-5 60-61 6.924440505207179E-5 3.4622202526035894E-5 0.0 3.527309993352537 6.924440505207179E-5 62-63 6.924440505207179E-5 3.4622202526035894E-5 0.0 3.9199777033015737 6.924440505207179E-5 64-65 6.924440505207179E-5 3.4622202526035894E-5 0.0 4.332449313095502 6.924440505207179E-5 66-67 6.924440505207179E-5 3.4622202526035894E-5 0.0 4.81902974739641 6.924440505207179E-5 68-69 6.924440505207179E-5 3.4622202526035894E-5 0.0 5.359499639929094 6.924440505207179E-5 70-71 6.924440505207179E-5 3.4622202526035894E-5 0.0 5.935197623532019 6.924440505207179E-5 72-73 6.924440505207179E-5 3.4622202526035894E-5 0.0 6.495661838023487 6.924440505207179E-5 74-75 6.924440505207179E-5 3.4622202526035894E-5 0.0 7.059899872590295 6.924440505207179E-5 76-77 6.924440505207179E-5 3.4622202526035894E-5 0.0 7.688933359184578 6.924440505207179E-5 78-79 6.924440505207179E-5 3.4622202526035894E-5 0.0 8.375803235098605 6.924440505207179E-5 80-81 6.924440505207179E-5 3.4622202526035894E-5 0.0 9.10712801905606 6.924440505207179E-5 82-83 6.924440505207179E-5 3.4622202526035894E-5 0.0 9.857720058719256 6.924440505207179E-5 84-85 6.924440505207179E-5 3.4622202526035894E-5 0.0 10.596280883004653 6.924440505207179E-5 86-87 6.924440505207179E-5 3.4622202526035894E-5 0.0 11.377686682916021 6.924440505207179E-5 88-89 6.924440505207179E-5 3.4622202526035894E-5 0.0 12.223420535120763 6.924440505207179E-5 90-91 6.924440505207179E-5 3.4622202526035894E-5 0.0 13.116984960115222 6.924440505207179E-5 92-93 6.924440505207179E-5 3.4622202526035894E-5 0.0 14.031149595612675 6.924440505207179E-5 94-95 6.924440505207179E-5 3.4622202526035894E-5 0.0 14.936520191668514 6.924440505207179E-5 96-97 6.924440505207179E-5 3.4622202526035894E-5 0.0 15.852866025925106 6.924440505207179E-5 98-99 6.924440505207179E-5 3.4622202526035894E-5 0.0 16.824313095501886 6.924440505207179E-5 100-101 6.924440505207179E-5 3.4622202526035894E-5 0.0 17.869089990028804 6.924440505207179E-5 102-103 6.924440505207179E-5 3.4622202526035894E-5 0.0 18.94012782517173 6.924440505207179E-5 104-105 6.924440505207179E-5 3.4622202526035894E-5 0.0 19.981269388433414 6.924440505207179E-5 106-107 6.924440505207179E-5 3.4622202526035894E-5 0.0 21.030512547086197 6.924440505207179E-5 108-109 6.924440505207179E-5 3.4622202526035894E-5 0.0 22.107436156658544 6.924440505207179E-5 110-111 6.924440505207179E-5 3.4622202526035894E-5 0.0 23.21901659095945 6.924440505207179E-5 112-113 6.924440505207179E-5 3.4622202526035894E-5 0.0 24.36800631508974 6.924440505207179E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9170 0.0 31.576889 1 CATGGGG 7555 0.0 30.820614 4 GTACATG 13290 0.0 28.915928 1 TACATGG 13140 0.0 28.59832 2 AACGCAG 9995 0.0 28.345377 6 TCAACGC 10425 0.0 27.019918 4 CAACGCA 10510 0.0 26.744734 5 ACATGGG 13670 0.0 26.661798 3 ATCAACG 10645 0.0 26.46196 3 TATCAAC 10875 0.0 26.449923 2 ACGCAGA 11245 0.0 24.980173 7 ATGGGGA 4305 0.0 24.899942 5 GGTATCA 6930 0.0 24.846601 1 CGCAGAG 11350 0.0 24.4332 8 GCAGAGT 12440 0.0 21.047476 9 GAGTACT 3820 0.0 20.94879 12-13 ATGGGGG 4180 0.0 19.945766 5 CTATACT 695 0.0 18.851107 4 GTACTTT 4360 0.0 18.217752 14-15 CGTACAC 360 4.6607965E-8 18.19687 3 >>END_MODULE