FastQCFastQC Report
Mon 15 Aug 2016
SRR2920919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888320
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85860.2972662308885442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73500.25447318856636386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52810.18283985153999557No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT33950.11754237757589188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84750.043.2166021
GTATCAA126050.039.751951
TATCAAC157400.031.5462132
ATCAACG157150.031.4827383
TCAACGC157500.031.3371774
CAACGCA158150.031.2078385
AACGCAG159950.031.0293086
CATGGGG79300.028.604724
GTACATG131650.027.4129961
TACATGG130700.027.3313792
ACGCAGA182500.026.9292817
CGCAGAG185750.026.2961258
ACATGGG138200.025.5034873
GAGTACT112800.024.8187912-13
GCAGAGT197350.023.9381689
AGTACTT119550.022.62113812-13
GTACTTT125600.022.09999314-15
AGAGTAC184150.021.8749510-11
CAGAGTA191100.021.42189610-11
ATGGGGG40400.020.6312275
Produced by FastQC (version 0.11.5)