FastQCFastQC Report
Mon 15 Aug 2016
SRR2920918_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920918_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824633
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33440.11838706125716154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84550.031.7863751
CATGGGG73150.029.4767594
GTACATG122600.029.1634461
TACATGG123900.028.124542
AACGCAG95550.027.4736376
TCAACGC94900.027.4284134
TATCAAC98950.026.7882962
ACATGGG125150.026.6537323
CAACGCA97900.026.5879125
ATCAACG98150.026.5818183
GGTATCA62950.024.423171
ACGCAGA106850.024.1764587
CGCAGAG108950.023.654578
GCAGAGT119300.020.8536729
ATGGGGA40000.019.8051035
GAGTACT37300.019.5422212-13
TAGGACG3051.6286504E-719.5292534
GTATAGG6150.019.3790721
ATGGGGG38450.019.0543545
GTATTAG9100.018.3355831
Produced by FastQC (version 0.11.5)