FastQCFastQC Report
Mon 15 Aug 2016
SRR2920918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824633
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86280.30545561140155203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74360.2632554388481618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48050.1701105948985231No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT32590.11537782076467987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83600.044.3172531
GTATCAA124300.038.973771
TCAACGC152100.031.6311194
ATCAACG152800.031.5262993
CAACGCA153550.031.2548685
TATCAAC155300.031.1721612
AACGCAG156650.030.7720386
ACGCAGA176550.027.1975987
CATGGGG69300.026.8932674
CGCAGAG181400.026.4015548
GAGTACT113700.026.1130212-13
GTACATG122450.025.9203191
TACATGG123850.025.048952
GCAGAGT193800.024.13039
ACATGGG124350.024.0862963
GTACTTT128950.023.0478914-15
AGTACTT120750.023.01159712-13
CAGAGTA187050.022.17134910-11
AGAGTAC180450.021.51496510-11
TACTTTT140300.019.95349514-15
Produced by FastQC (version 0.11.5)