##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920918_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2824633
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.024718255433537	31.0	31.0	34.0	28.0	34.0
2	31.141289859603	33.0	31.0	34.0	27.0	34.0
3	31.216016381597186	33.0	31.0	34.0	27.0	34.0
4	34.74597160055837	37.0	35.0	37.0	32.0	37.0
5	34.440377210065876	37.0	35.0	37.0	30.0	37.0
6	34.13919578224853	37.0	35.0	37.0	30.0	37.0
7	34.04061554191288	37.0	35.0	37.0	30.0	37.0
8	34.0382704584985	37.0	35.0	37.0	30.0	37.0
9	35.568817612765976	39.0	35.0	39.0	30.0	39.0
10-11	35.533885110030226	39.0	35.0	39.0	30.0	39.0
12-13	35.4259625940786	39.0	35.0	39.0	29.0	39.0
14-15	36.61609083374725	40.0	36.0	41.0	29.0	41.0
16-17	36.62918492420077	40.0	36.5	41.0	29.5	41.0
18-19	36.68267913035074	40.0	37.0	41.0	30.0	41.0
20-21	36.687917509991564	40.0	37.0	41.0	30.0	41.0
22-23	36.631144293789674	40.0	37.0	41.0	30.0	41.0
24-25	36.60558398206068	40.0	36.5	41.0	30.0	41.0
26-27	36.457439603658244	40.0	36.0	41.0	29.0	41.0
28-29	36.33473693750658	40.0	36.0	41.0	29.0	41.0
30-31	36.149912749727136	40.0	36.0	41.0	28.0	41.0
32-33	35.955670701291105	40.0	35.0	41.0	27.5	41.0
34-35	35.80117009891197	40.0	35.0	41.0	27.0	41.0
36-37	35.64037221826694	40.0	35.0	41.0	26.0	41.0
38-39	35.492948818483676	40.0	35.0	41.0	26.0	41.0
40-41	35.33174982378242	40.0	35.0	41.0	25.0	41.0
42-43	35.156362968215696	39.0	35.0	41.0	24.5	41.0
44-45	34.917800825806395	39.0	35.0	41.0	23.5	41.0
46-47	34.637018331231	39.0	34.5	41.0	22.5	41.0
48-49	34.32083796372839	39.0	34.0	41.0	19.0	41.0
50-51	34.10425973922984	39.0	34.0	41.0	17.5	41.0
52-53	33.89733763642923	39.0	33.5	41.0	15.0	41.0
54-55	33.68071303422427	38.0	33.0	41.0	13.5	41.0
56-57	33.42255843502501	38.0	33.0	40.0	10.5	41.0
58-59	33.19100906206222	37.5	33.0	40.0	8.0	41.0
60-61	32.91029507196156	37.0	33.0	40.0	7.5	41.0
62-63	32.609305704493295	36.5	32.0	40.0	6.5	41.0
64-65	32.2965224154784	36.0	32.0	39.5	2.0	41.0
66-67	31.961511991115305	35.5	32.0	39.0	2.0	41.0
68-69	31.58883791274831	35.0	31.0	39.0	2.0	41.0
70-71	31.24723211829643	35.0	31.0	38.0	2.0	40.0
72-73	30.887466088514863	35.0	31.0	37.0	2.0	39.5
74-75	30.565536301530145	35.0	31.0	37.0	2.0	39.0
76-77	30.360438329510416	35.0	30.5	36.0	2.0	39.0
78-79	30.21536585460837	35.0	31.0	36.0	2.0	38.5
80-81	29.974429244436358	35.0	31.0	35.5	2.0	37.0
82-83	29.756717067314586	35.0	30.5	35.0	2.0	37.0
84-85	29.52166865571563	35.0	30.0	35.0	2.0	36.5
86-87	29.356255131197575	35.0	30.0	35.0	2.0	36.0
88-89	29.20265783200862	35.0	30.0	35.0	2.0	36.0
90-91	29.041301294716874	34.5	30.0	35.0	2.0	35.5
92-93	28.919957920197067	34.0	30.0	35.0	2.0	35.0
94-95	28.83111186479801	34.0	30.0	35.0	2.0	35.0
96-97	28.763771789113843	34.0	29.5	35.0	2.0	35.0
98-99	28.658957818590945	34.0	29.0	35.0	2.0	35.0
100-101	28.55975714367141	34.0	29.0	35.0	2.0	35.0
102-103	27.17117073262261	32.5	26.0	34.0	2.0	35.0
104-105	28.218714254205768	34.0	29.0	35.0	2.0	35.0
106-107	28.3902108698723	34.0	29.0	35.0	2.0	35.0
108-109	28.484607734880957	34.0	29.5	35.0	2.0	35.0
110-111	28.646628075222516	35.0	30.0	35.0	2.0	35.0
112-113	28.608487368093485	35.0	30.0	35.0	2.0	35.0
114-115	28.512857068511202	35.0	30.0	35.0	2.0	35.0
116-117	28.39043727096582	35.0	30.0	35.0	2.0	35.0
118-119	28.280105061436302	35.0	30.0	35.0	2.0	35.0
120-121	28.090107104179552	35.0	29.0	35.0	2.0	35.0
122-123	27.88950599954047	35.0	29.0	35.0	2.0	35.0
124-125	27.151510125386203	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57543.0
3	22801.0
4	9758.0
5	8699.0
6	10212.0
7	12468.0
8	13528.0
9	14989.0
10	17195.0
11	19260.0
12	20900.0
13	24206.0
14	30637.0
15	27926.0
16	16227.0
17	13951.0
18	12827.0
19	12575.0
20	12152.0
21	12622.0
22	12862.0
23	13959.0
24	18301.0
25	24990.0
26	30624.0
27	35976.0
28	43340.0
29	51625.0
30	61188.0
31	75088.0
32	93298.0
33	118510.0
34	159283.0
35	236707.0
36	435787.0
37	747717.0
38	293954.0
39	948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08795267620744	18.90478939117163	12.943833506757882	25.063424425863047
2	16.366926533897043	20.782320861382154	37.93200020818647	24.918752396534334
3	18.993386721045663	24.816789515049813	29.945550197902726	26.244273566001798
4	13.267678525340662	16.817102490962345	35.44078021051693	34.47443877318007
5	13.573289228274858	37.56136725681276	34.27892764616178	14.586415868750603
6	32.72599879704404	36.262745984302065	16.442452584902973	14.568802633750922
7	27.401717458920054	32.772415268237424	21.265213860783767	18.56065341205876
8	26.351356614811756	34.844920369298926	19.837424340388406	18.96629867550091
9	26.460461509590296	15.040382974353891	19.467001391539583	39.03215412451624
10-11	25.22988241209233	25.82646135107479	27.60159262393468	21.342063612898198
12-13	26.02792668338281	23.394771611878905	27.791548558688756	22.785753146049533
14-15	23.306430509162386	24.62403385846485	25.59220266636827	26.477332966004496
16-17	22.679301641431245	27.486240253974326	26.5412376290654	23.293220475529033
18-19	22.691589857324697	25.957322943083387	28.94962498178513	22.401462217806785
20-21	23.51110004773148	25.284813174455017	28.82910572168723	22.374981056126273
22-23	23.16255611514065	25.17805387018094	28.608263073409233	23.051126941269175
24-25	22.87523311490374	25.4034786568191	28.811851354571534	22.90943687370563
26-27	22.75156207787959	25.478289534016245	28.841868892033833	22.92827949607033
28-29	22.726425347649762	25.504897025349884	28.9616989077848	22.806978719215557
30-31	23.039485750501893	25.373099978401804	28.772425831442018	22.81498843965429
32-33	22.635429885786802	25.595002039521393	28.733634257160983	23.035933817530818
34-35	22.85899779948802	25.67343651490174	28.77801917095813	22.689546514652108
36-37	23.124532922532374	25.321003166875965	28.58970003114292	22.964763879448732
38-39	22.99260205657976	25.384827443584996	28.590927528908427	23.031642970926825
40-41	23.08404218664293	25.39876207848149	28.57311821445809	22.944077520417487
42-43	23.127033120037762	25.36275031068349	28.423870194763452	23.086346374515294
44-45	23.011756465772894	25.578784892546665	28.20170796473025	23.207750676950194
46-47	23.103356515559344	25.604529908824997	27.86406661722274	23.42804695839292
48-49	23.216593985269913	25.778823466090856	27.46427292252513	23.5403096261141
50-51	23.067204849809336	26.02098616826361	27.39930886463693	23.512500117290124
52-53	23.143272420837953	25.94861681071569	27.2837228735213	23.62438789492506
54-55	22.94998086994056	26.11480620568151	27.10485981411	23.83035311026793
56-57	22.983512649638538	26.18935780583202	26.865505423398297	23.961624121131152
58-59	22.830197331159667	25.997176810185014	27.16593419414715	24.00669166450817
60-61	22.920333112136753	26.025129300851198	27.211322526570147	23.843215060441906
62-63	22.75669633569315	26.060275652989784	27.288817340276744	23.894210671040323
64-65	22.620430284956313	26.04379051316499	27.194075809413466	24.14170339246523
66-67	22.70794879217559	26.187573754440756	27.01479205342101	24.08968539996264
68-69	22.92396600630769	25.83359860291746	27.050893872927666	24.191541517847185
70-71	22.623117531605075	25.78544844096509	27.150814527976724	24.44061949945311
72-73	22.75018338911585	25.831634007203125	26.98891095637313	24.429271647307893
74-75	22.669953257609315	25.87161758556308	26.92472885270641	24.533700304121197
76-77	22.666381751805044	25.69144818128658	27.06010175142972	24.58206831547865
78-79	22.68682749510966	25.733276750727953	27.028037601584455	24.551858152577935
80-81	22.653858104333953	25.776139921622505	26.871990800780054	24.698011173263485
82-83	22.41534034790297	25.76483187038608	26.974013942391018	24.845813839319934
84-85	22.50796349750862	25.753717243078945	26.76317641926593	24.975142840146507
86-87	22.366162781268933	25.646316145802533	26.879637947524206	25.107883125404328
88-89	22.36206456373781	25.553354652272432	26.835890319476025	25.24869046451374
90-91	22.358635971094635	25.65738668411554	26.711471466240077	25.272505878549744
92-93	22.321225137172544	25.59049298199553	26.798238058911828	25.290043821920094
94-95	22.084857420505365	25.493678545827425	26.891156054310706	25.530307979356508
96-97	22.174869831085804	25.36887241233405	26.906467814950346	25.549789941629797
98-99	22.089581304771176	25.482853855005754	26.712968044613618	25.714596795609452
100-101	22.046198976082568	25.491195857448968	26.744297007935298	25.718308158533166
102-103	21.881761247706265	25.37361924477284	26.811899300211966	25.93272020730893
104-105	21.756644726325206	25.57278064042949	26.655133839859523	26.015440793385785
106-107	21.662770265270407	25.65740487983116	26.714868037506008	25.96495681739243
108-109	21.56597321930926	25.660886502686246	26.7847272801952	25.98841299780929
110-111	21.52259301242585	25.866958466190106	26.64576719262929	25.964681328754757
112-113	21.474223761756804	25.9487720201411	26.55586219178795	26.02114202631414
114-115	21.327632633414982	26.07448852404283	26.519212511752293	26.078666330789886
116-117	21.219376967599313	26.03653450441634	26.587480014218407	26.15660851376594
118-119	21.176083438084106	26.07576432660432	26.49336897072342	26.25478326458815
120-121	21.096346779368485	26.11806253180802	26.569601486955946	26.215989201867547
122-123	21.009123480305036	26.21267586685454	26.406970239823263	26.371230413017155
124-125	20.792174375437668	26.319576664879996	26.385833411810022	26.502415547872317
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	187.0
1	165.5
2	326.0
3	559.5
4	406.5
5	256.0
6	468.0
7	1023.5
8	1775.0
9	1992.0
10	1560.5
11	1258.5
12	1350.5
13	1509.5
14	1683.5
15	1909.0
16	2152.0
17	2435.0
18	2722.0
19	3136.5
20	3636.5
21	4210.0
22	5108.5
23	6330.5
24	7731.5
25	9471.0
26	12051.5
27	15337.0
28	18575.5
29	22531.0
30	26670.5
31	31301.0
32	36888.5
33	40332.0
34	43020.0
35	46311.5
36	50763.0
37	55613.0
38	60329.5
39	65423.5
40	70566.5
41	75698.0
42	80243.0
43	86594.5
44	93643.0
45	99434.5
46	104281.5
47	108517.5
48	113090.0
49	116458.0
50	119557.5
51	119427.5
52	114050.0
53	110680.0
54	108984.5
55	104194.5
56	96626.0
57	88017.5
58	80522.5
59	71442.0
60	59710.0
61	49354.0
62	40143.5
63	30545.0
64	22626.0
65	17247.5
66	12841.5
67	9836.5
68	7561.0
69	5689.5
70	4468.5
71	3449.0
72	2677.5
73	2322.0
74	1829.5
75	1309.0
76	962.5
77	585.0
78	345.0
79	221.5
80	130.0
81	71.0
82	49.0
83	37.5
84	26.0
85	24.0
86	25.5
87	17.5
88	10.5
89	9.5
90	8.0
91	5.0
92	5.0
93	5.5
94	5.5
95	11.0
96	13.0
97	6.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2097263609113113
2	0.008567484696241955
3	4.6023678120308024E-4
4	0.0021949754180454594
5	0.08075385368647892
6	0.056184290136099095
7	0.028676291751884228
8	0.01667473261128083
9	0.014975396803761762
10-11	0.01285122704436293
12-13	0.014745278413160222
14-15	0.0227286164255675
16-17	0.025684752674064202
18-19	0.024905890428951305
20-21	0.017736817490980245
22-23	0.014780681242483535
24-25	0.013523880801505895
26-27	0.01239099026315985
28-29	0.01481608407180685
30-31	0.011435113871430378
32-33	0.015612647731581412
34-35	0.018320964174814925
36-37	0.019701674518424164
38-39	0.02380840271992857
40-41	0.024994397502259583
42-43	0.02186124710714631
44-45	0.020179612714288902
46-47	0.01977248017707079
48-49	0.019453854713160967
50-51	0.015559543487596441
52-53	0.01285122704436293
54-55	0.019241437737221083
56-57	0.021932052765792936
58-59	0.018497978321431494
60-61	0.021206294764665005
62-63	0.026782240383086936
64-65	0.019506958957145937
66-67	0.023029540474815667
68-69	0.016356107147371002
70-71	0.01706416373383728
72-73	0.02547233569812432
74-75	0.01522321660902496
76-77	0.01407262465601726
78-79	0.015701154804889695
80-81	0.01701105948985231
82-83	0.013045942605641157
84-85	0.017595206173686987
86-87	0.018126248613536698
88-89	0.019117527834589483
90-91	0.013523880801505895
92-93	0.012798122800377962
94-95	0.014214235973310514
96-97	0.014798382657145193
98-99	0.01355928363082921
100-101	0.016975656660528998
102-103	0.022480796620304302
104-105	0.008195754988347159
106-107	0.008213456403008816
108-109	0.015364827926318214
110-111	0.011311203968798778
112-113	0.009151631380076634
114-115	0.006531822010151407
116-117	0.004425353665414232
118-119	0.002531302296616941
120-121	0.002495899467293627
122-123	0.0018763499541356347
124-125	5.310424398497079E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2824633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.54438372401409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.35706669728748	31.132804759092856
2	13.973487713684255	12.728277727896643
3	6.386788897514831	8.726470929380651
4	3.3948522327644217	6.1846581110139525
5	2.100586338872043	4.783495513150512
6	1.3289513427999646	3.631576194421523
7	0.9103106023485377	2.9021674766980143
8	0.6668931600373119	2.429859038692774
9	0.48480023035835806	1.987193494883834
>10	2.25570585769473	17.427406896523337
>50	0.10023000924521124	3.1103206378985173
>100	0.037140581446435554	2.9529402882774374
>500	0.0024091808375596244	0.7666921887100467
>1k	6.217240870941095E-4	0.6675506522667781
>5k	1.5543102177352738E-4	0.5685860910929758
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8628	0.30545561140155203	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7436	0.2632554388481618	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4805	0.1701105948985231	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	3259	0.11537782076467987	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	3.5402829323313864E-5	0.0
14-15	0.0	0.0	0.0	3.5402829323313864E-5	1.7701414661656932E-5
16-17	0.0	0.0	0.0	3.5402829323313864E-5	3.5402829323313864E-5
18-19	0.0	0.0	0.0	8.850707330828467E-5	7.080565864662773E-5
20-21	0.0	0.0	0.0	2.1241697593988315E-4	1.0620848796994158E-4
22-23	0.0	0.0	0.0	4.7793819586473715E-4	1.0620848796994158E-4
24-25	0.0	0.0	0.0	0.0012036961969926713	1.0620848796994158E-4
26-27	0.0	0.0	0.0	0.0026198093699252257	1.2390990263159852E-4
28-29	0.0	0.0	0.0	0.007292982840602655	1.4161131729325545E-4
30-31	0.0	0.0	0.0	0.025313022966169413	1.4161131729325545E-4
32-33	0.0	0.0	0.0	0.0670706601530181	1.4161131729325545E-4
34-35	0.0	0.0	0.0	0.12902561146881736	1.4161131729325545E-4
36-37	0.0	0.0	0.0	0.2317646221650742	1.4161131729325545E-4
38-39	0.0	0.0	0.0	0.3875193697729935	1.4161131729325545E-4
40-41	0.0	0.0	0.0	0.555293377936178	1.4161131729325545E-4
42-43	0.0	0.0	0.0	0.7137387405726692	1.4161131729325545E-4
44-45	0.0	0.0	0.0	0.8965058469542768	1.4161131729325545E-4
46-47	0.0	0.0	0.0	1.119862297155064	1.4161131729325545E-4
48-49	0.0	0.0	0.0	1.3510250712216418	1.4161131729325545E-4
50-51	0.0	0.0	0.0	1.5864361848070174	1.4161131729325545E-4
52-53	0.0	0.0	0.0	1.816997110775099	1.4161131729325545E-4
54-55	0.0	0.0	0.0	2.0725701356601016	1.4161131729325545E-4
56-57	0.0	0.0	0.0	2.3770521692552626	1.4161131729325545E-4
58-59	0.0	0.0	0.0	2.7000498825865167	1.4161131729325545E-4
60-61	0.0	0.0	0.0	3.035102259302359	1.4161131729325545E-4
62-63	0.0	0.0	0.0	3.36629572762196	1.4161131729325545E-4
64-65	0.0	0.0	0.0	3.7170492591426925	1.4161131729325545E-4
66-67	0.0	0.0	0.0	4.123243621383734	1.4161131729325545E-4
68-69	0.0	0.0	0.0	4.581745663949972	1.4161131729325545E-4
70-71	0.0	0.0	0.0	5.065507625238394	1.4161131729325545E-4
72-73	0.0	0.0	0.0	5.54183499236892	1.4161131729325545E-4
74-75	0.0	0.0	0.0	6.026836052683658	1.4161131729325545E-4
76-77	0.0	0.0	0.0	6.564711238592766	1.4161131729325545E-4
78-79	0.0	0.0	0.0	7.156133203853385	1.770141466165693E-4
80-81	0.0	0.0	0.0	7.777806886770777	1.770141466165693E-4
82-83	0.0	0.0	0.0	8.420669163038172	1.770141466165693E-4
84-85	0.0	0.0	0.0	9.069178190582635	1.770141466165693E-4
86-87	0.0	0.0	0.0	9.74324806089853	1.770141466165693E-4
88-89	0.0	0.0	0.0	10.480635891459173	1.770141466165693E-4
90-91	0.0	0.0	0.0	11.256736715884859	1.770141466165693E-4
92-93	0.0	0.0	0.0	12.056504331713182	1.770141466165693E-4
94-95	0.0	0.0	0.0	12.840623896980599	1.770141466165693E-4
96-97	0.0	0.0	0.0	13.651631911119072	1.770141466165693E-4
98-99	0.0	0.0	0.0	14.506716447765072	1.770141466165693E-4
100-101	0.0	0.0	0.0	15.437651546236273	1.770141466165693E-4
102-103	0.0	0.0	0.0	16.383455833023262	1.770141466165693E-4
104-105	0.0	0.0	0.0	17.328074125027925	1.770141466165693E-4
106-107	0.0	0.0	0.0	18.269630072296117	1.770141466165693E-4
108-109	0.0	0.0	0.0	19.233330489306045	1.770141466165693E-4
110-111	0.0	0.0	0.0	20.259269080266357	1.770141466165693E-4
112-113	0.0	0.0	0.0	21.30958251921577	1.770141466165693E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8360	0.0	44.317253	1
GTATCAA	12430	0.0	38.97377	1
TCAACGC	15210	0.0	31.631119	4
ATCAACG	15280	0.0	31.526299	3
CAACGCA	15355	0.0	31.254868	5
TATCAAC	15530	0.0	31.172161	2
AACGCAG	15665	0.0	30.772038	6
ACGCAGA	17655	0.0	27.197598	7
CATGGGG	6930	0.0	26.893267	4
CGCAGAG	18140	0.0	26.401554	8
GAGTACT	11370	0.0	26.11302	12-13
GTACATG	12245	0.0	25.920319	1
TACATGG	12385	0.0	25.04895	2
GCAGAGT	19380	0.0	24.1303	9
ACATGGG	12435	0.0	24.086296	3
GTACTTT	12895	0.0	23.04789	14-15
AGTACTT	12075	0.0	23.011597	12-13
CAGAGTA	18705	0.0	22.171349	10-11
AGAGTAC	18045	0.0	21.514965	10-11
TACTTTT	14030	0.0	19.953495	14-15
>>END_MODULE