FastQCFastQC Report
Mon 15 Aug 2016
SRR2920917_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920917_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3317481
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG147050.028.081431
GTATCAA97050.027.9975681
TACATGG148700.027.1180322
CATGGGG87550.027.0757924
ACATGGG151350.026.0135443
AACGCAG105500.024.8823456
TCAACGC107300.024.6454934
CAACGCA108150.024.4517945
TATCAAC111650.023.9534532
ATCAACG111400.023.8468043
ACGCAGA120750.021.7855347
CGCAGAG121600.021.5334138
GTACTAG6650.021.5050581
GGTATCA74000.020.9359471
ATGGGGG47650.018.4992055
GCAGAGT136050.018.4589799
ATACCGT3905.86806E-918.3156286
GTATAGG7900.018.1023581
ATGGGGA43850.017.5216245
TAGACAG17600.017.2588465
Produced by FastQC (version 0.11.5)