##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920917_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3317481
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.700473943935172	31.0	30.0	34.0	25.0	34.0
2	29.739639805020737	31.0	30.0	34.0	25.0	34.0
3	29.850090173839728	31.0	30.0	34.0	25.0	34.0
4	33.26004127830725	37.0	35.0	37.0	27.0	37.0
5	33.347888955505695	37.0	35.0	37.0	27.0	37.0
6	33.37131154632084	37.0	35.0	37.0	27.0	37.0
7	33.25057294977726	37.0	35.0	37.0	26.0	37.0
8	33.17302525621096	37.0	35.0	37.0	26.0	37.0
9	34.58884617575805	39.0	35.0	39.0	26.0	39.0
10-11	34.60676850899824	39.0	35.0	39.0	27.0	39.0
12-13	34.560695449348465	39.0	35.0	39.0	26.5	39.0
14-15	35.6967061755591	40.0	36.0	41.0	26.5	41.0
16-17	35.684064053418844	40.0	36.0	41.0	26.5	41.0
18-19	35.66935861878335	40.0	36.0	41.0	26.0	41.0
20-21	35.70974860142379	40.0	36.0	41.0	26.0	41.0
22-23	35.62783584894684	40.0	36.0	41.0	26.0	41.0
24-25	35.63821420529612	40.0	36.0	41.0	26.0	41.0
26-27	35.58022050465398	40.0	35.5	41.0	26.0	41.0
28-29	35.4710659985694	40.0	35.0	41.0	25.5	41.0
30-31	35.3283239602578	40.0	35.0	41.0	25.0	41.0
32-33	35.20607277027359	40.0	35.0	41.0	25.0	41.0
34-35	35.14319584648714	40.0	35.0	41.0	24.5	41.0
36-37	34.995252421943036	40.0	35.0	41.0	24.0	41.0
38-39	34.85074202384279	39.0	35.0	41.0	23.5	41.0
40-41	34.720758611729806	39.0	34.0	41.0	23.0	41.0
42-43	34.51783898686986	39.0	34.0	41.0	22.0	41.0
44-45	34.350529814639486	39.0	34.0	41.0	21.0	41.0
46-47	34.14553964287964	39.0	33.0	41.0	19.5	41.0
48-49	34.01007225060219	39.0	33.0	40.5	18.5	41.0
50-51	33.80770620841536	38.0	33.0	40.0	17.5	41.0
52-53	33.61209212652612	38.0	33.0	40.0	15.5	41.0
54-55	33.37158404222963	38.0	33.0	40.0	13.5	41.0
56-57	33.102160796098005	38.0	32.0	40.0	10.5	41.0
58-59	32.837468549179334	37.0	32.0	40.0	8.0	41.0
60-61	32.56368597137406	37.0	31.5	40.0	7.5	41.0
62-63	32.24141253559553	36.0	31.0	40.0	7.0	41.0
64-65	31.951137625204183	36.0	31.0	39.0	4.0	41.0
66-67	31.593635803792093	35.0	31.0	39.0	2.0	40.5
68-69	31.255988655247762	35.0	30.5	38.5	2.0	40.0
70-71	30.904182872486686	35.0	30.0	38.0	2.0	40.0
72-73	30.963470777978834	35.0	30.5	37.0	2.0	40.0
74-75	30.95859946145886	35.0	31.0	37.0	2.0	39.0
76-77	30.701455562217237	35.0	31.0	36.5	2.0	39.0
78-79	30.415875177582027	35.0	31.0	36.0	2.0	39.0
80-81	30.150366196520793	35.0	31.0	36.0	2.0	37.0
82-83	29.9178955357996	35.0	31.0	35.0	2.0	37.0
84-85	29.688361591219362	35.0	30.0	35.0	2.0	36.5
86-87	29.49271374877505	35.0	30.0	35.0	2.0	36.0
88-89	29.30151596949613	35.0	30.0	35.0	2.0	36.0
90-91	29.135987666545795	34.0	30.0	35.0	2.0	36.0
92-93	28.974871295419625	34.0	30.0	35.0	2.0	35.0
94-95	28.861018797093337	34.0	30.0	35.0	2.0	35.0
96-97	28.764477626247142	34.0	29.5	35.0	2.0	35.0
98-99	28.629957790263155	34.0	29.0	35.0	2.0	35.0
100-101	28.49963797833356	34.0	29.0	35.0	2.0	35.0
102-103	27.121586679772996	32.5	26.0	34.5	2.0	35.0
104-105	28.137838769837717	34.0	29.0	35.0	2.0	35.0
106-107	28.284597560619037	34.0	29.0	35.0	2.0	35.0
108-109	28.206436299107665	34.0	29.0	35.0	2.0	35.0
110-111	28.09541923525711	34.0	29.0	35.0	2.0	35.0
112-113	27.965843511989974	34.0	29.0	35.0	2.0	35.0
114-115	27.846030165658824	34.0	29.0	35.0	2.0	35.0
116-117	27.6190143967667	34.0	28.0	35.0	2.0	35.0
118-119	27.40235573316019	34.0	27.0	35.0	2.0	35.0
120-121	27.211789607837993	34.0	27.0	35.0	2.0	35.0
122-123	26.900562806539057	34.0	27.0	35.0	2.0	35.0
124-125	25.6050765626088	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	128292.0
3	19068.0
4	12764.0
5	11992.0
6	16022.0
7	16674.0
8	15741.0
9	16760.0
10	18062.0
11	19655.0
12	19943.0
13	20126.0
14	19836.0
15	17684.0
16	14166.0
17	12938.0
18	12453.0
19	12609.0
20	13374.0
21	14414.0
22	16960.0
23	22419.0
24	30407.0
25	38661.0
26	42715.0
27	47147.0
28	55238.0
29	64567.0
30	76899.0
31	93568.0
32	117102.0
33	150320.0
34	204136.0
35	299093.0
36	531431.0
37	816918.0
38	276960.0
39	367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46900784525903	18.233252739077503	11.936635282410002	25.361104133253466
2	16.049668929147668	20.9064413543492	38.323258576372695	24.720631140130436
3	19.259073845326064	24.66580452542627	28.93488317462829	27.140238454619375
4	12.924058744816	16.426464688724497	35.95763993217898	34.69183663428053
5	13.693317773728522	38.19915187070029	33.96889186124979	14.138638494321398
6	32.76232852882629	36.17842989872485	17.050664128778127	14.008577443670733
7	28.088369082589153	32.73400713023773	21.05951772073789	18.118106066435228
8	26.11277504277496	35.61100385314076	19.847702733403754	18.42851837068053
9	26.2611779593588	16.26251462258273	19.119292817652887	38.357014600405584
10-11	25.026299807853768	26.62229915982409	27.43307539861518	20.918325633706957
12-13	25.34111408075969	24.11187564479249	27.397376184759064	23.14963408968875
14-15	22.872147638707666	25.517094296189924	24.659789019041558	26.95096904606085
16-17	23.02676122765995	28.252733716365587	25.57181462598398	23.14869042999048
18-19	23.187257125146015	27.46322705975598	26.90086218923519	22.44865362586281
20-21	23.950545302304423	26.64282178747915	26.608390690844946	22.79824221937148
22-23	23.642489548563702	26.50691147503011	26.356439746613937	23.494159229792256
24-25	23.29220853842193	26.65995463041524	26.508737762171318	23.539099068991508
26-27	23.20367576770292	26.736899481979066	26.54767539124562	23.511749359072397
28-29	23.22930931510651	26.89561301376061	26.680335780602427	23.19474189053046
30-31	23.528753516361327	26.79951058614593	26.421109162122335	23.250626735370407
32-33	23.07825149742866	26.93545095705272	26.41232940368758	23.57396814183104
34-35	23.27180540752177	27.047155758049513	26.499363314390873	23.181675520037835
36-37	23.499695939342313	26.778694517979396	26.401182699431214	23.320426843247077
38-39	23.378398718410065	26.904822160091964	26.413578193183817	23.303200928314155
40-41	23.404333298995457	26.861910916291855	26.485164605363554	23.24859117934913
42-43	23.33490995461382	26.849307382629696	26.38051392625153	23.43526873650496
44-45	23.18567518605946	26.946619017427487	26.488023242275748	23.379682554237306
46-47	23.188704794815866	26.965355416779964	26.40728208408908	23.438657704315087
48-49	23.17009393599227	26.979996754795877	26.407169970883643	23.442739338328213
50-51	23.029713326750077	27.074479668416235	26.377250728551687	23.518556276282002
52-53	23.15875626089228	26.964485968905315	26.268511218179768	23.60824655202264
54-55	23.058634180079288	26.902493938474898	26.319890775668597	23.718981105777214
56-57	23.07066415324379	27.056859531503726	26.234165488656796	23.638310826595692
58-59	23.016027765273954	26.795148164652353	26.422265300721353	23.766558769352336
60-61	23.175016118268502	26.826409075999052	26.460367911260917	23.53820689447153
62-63	23.05115127042327	26.757738186171302	26.571747550399422	23.61936299300601
64-65	22.974558600901744	26.762410770525552	26.458475995184692	23.80455463338801
66-67	22.980348192531974	26.994406913903752	26.320606528926664	23.704638364637603
68-69	23.15081985458622	26.77378281073482	26.336434534769264	23.738962799909697
70-71	23.02928799119071	26.6857703498411	26.449990363708913	23.834951295259277
72-73	23.05951662159888	26.77082321745996	26.392009520632765	23.777650640308394
74-75	22.93027450164297	26.911958306754798	26.405480683245642	23.752286508356594
76-77	23.019706227674682	26.70177637318843	26.489232947925878	23.789284451211007
78-79	23.06242723658891	26.78138402794115	26.48025660978302	23.675932125686916
80-81	23.062082955824533	26.737274384454558	26.413380882351166	23.78726177736974
82-83	23.014355582299157	26.686528313780844	26.488620648572507	23.81049545534749
84-85	23.08241610827325	26.74144319996187	26.359783669460256	23.81635702230462
86-87	23.088443178915245	26.605233005459933	26.493534068703518	23.812789746921304
88-89	23.02194107576221	26.53122837421118	26.4694603334117	23.97737021661491
90-91	23.08713020896954	26.567241381910417	26.479337546805887	23.866290862314152
92-93	23.109927858805932	26.632737384338846	26.42617591352708	23.831158843328147
94-95	22.948436608564656	26.55885578969323	26.579758216531346	23.912949385210762
96-97	23.153441326742566	26.428423114974574	26.474776230110496	23.943359328172363
98-99	23.06600586362923	26.575618515171534	26.261884344188367	24.096491277010866
100-101	22.901714581945782	26.712791352194454	26.462439981187835	23.92305408467193
102-103	22.860144778062057	26.74369687078178	26.432822713822674	23.96333563733349
104-105	22.796082688741123	26.902579876660486	26.278192427737824	24.02314500686057
106-107	22.80475053164201	26.955222638192993	26.274048299725756	23.965978530439237
108-109	22.849250881686007	26.961005855688956	26.230684678742378	23.95905858388266
110-111	22.80161938435344	27.229288868881596	26.130315348635897	23.838776398129067
112-113	22.82232968199665	27.359146300542687	26.127233009672135	23.69129100778853
114-115	22.82503192693934	27.493332593348217	25.985979896132633	23.69565558357981
116-117	22.802331591331143	27.424929036196854	26.03854645499211	23.73419291747989
118-119	22.898698210467312	27.384466386334744	25.928852717763323	23.78798268543462
120-121	22.926499472768555	27.58194105624848	25.85227204177314	23.63928742920983
122-123	22.95516485478615	27.741693319065103	25.719132179408145	23.584009646740604
124-125	23.089644425487656	27.864206118470943	25.48384051120586	23.562308944835543
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2471.0
1	1859.0
2	1063.0
3	818.5
4	673.0
5	556.5
6	539.5
7	588.5
8	658.0
9	687.0
10	670.0
11	698.0
12	809.0
13	930.5
14	1066.5
15	1237.5
16	1402.5
17	1641.5
18	1954.5
19	2343.0
20	2821.0
21	3475.5
22	4491.5
23	5852.0
24	7452.0
25	9397.0
26	12603.5
27	16426.0
28	19627.0
29	23513.0
30	28750.0
31	34542.0
32	40416.5
33	45977.5
34	51463.5
35	57611.0
36	64711.0
37	71186.0
38	77334.0
39	83391.5
40	88607.0
41	94173.5
42	99431.5
43	107080.0
44	114884.0
45	120537.0
46	125724.0
47	129250.5
48	133773.5
49	138383.0
50	142161.5
51	140960.0
52	134513.0
53	130990.0
54	127314.5
55	119759.0
56	109883.5
57	98940.5
58	89908.5
59	80055.5
60	67455.5
61	55500.0
62	45138.0
63	34931.0
64	25736.5
65	19427.5
66	14646.0
67	11389.0
68	8875.0
69	6325.0
70	4801.0
71	3684.0
72	2735.0
73	2181.5
74	1673.5
75	1247.0
76	905.0
77	578.0
78	357.5
79	245.5
80	172.0
81	124.5
82	103.0
83	87.0
84	68.0
85	50.5
86	38.0
87	30.5
88	29.5
89	26.5
90	18.5
91	14.5
92	11.5
93	9.0
94	9.5
95	6.5
96	6.5
97	8.5
98	9.0
99	9.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16328654180687094
2	0.14306035211656074
3	0.16795876148197986
4	0.13998573013681165
5	0.16536643314611296
6	0.10369313343467527
7	0.10327112649627834
8	0.0916960790431053
9	0.09820704323551516
10-11	0.08494396802875434
12-13	0.07418279109963252
14-15	0.07362513907389372
16-17	0.07362513907389372
18-19	0.07154524773465168
20-21	0.065230215335069
22-23	0.05751351703295361
24-25	0.06161301300595241
26-27	0.07125888588359662
28-29	0.06694838644139936
30-31	0.06289410549751452
32-33	0.07178639455659279
34-35	0.06828976563844677
36-37	0.0709273090034276
38-39	0.06703881649962729
40-41	0.07190696796756334
42-43	0.07159046276376564
44-45	0.06459720492747359
46-47	0.06198980491523538
48-49	0.05483075863885882
50-51	0.06025656213253369
52-53	0.062065163297091976
54-55	0.06337639914139674
56-57	0.06675245464857221
58-59	0.05511712048991388
60-61	0.06461227660384491
62-63	0.06003048698696391
64-65	0.054333393318605296
66-67	0.05905082802282817
68-69	0.06165822803506636
70-71	0.05697093668358613
72-73	0.052298717008477213
74-75	0.05534319563548368
76-77	0.06506442689498447
78-79	0.058508247673460675
80-81	0.05682021991987294
82-83	0.06167329971143769
84-85	0.06304482226122772
86-87	0.056428356334218645
88-89	0.05578027425025193
90-91	0.0580560973823211
92-93	0.05308244417978581
94-95	0.06259267197008815
96-97	0.04914873664687153
98-99	0.04872672970847459
100-101	0.046465978252776735
102-103	0.04548631928864099
104-105	0.04313513777471521
106-107	0.05455946846417508
108-109	0.05475540025700222
110-111	0.06099507427472832
112-113	0.06114579103844151
114-115	0.0632708974067975
116-117	0.06735532170342498
118-119	0.060482637278103484
120-121	0.06176372976966559
122-123	0.05899054131734289
124-125	0.07050530206503067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3317481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.785848509306774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.18759044878807	31.902142769516523
2	14.153530732593042	13.243698894538314
3	6.316508384805633	8.86569613197847
4	3.48755106082674	6.52672142401248
5	2.073998753325654	4.851689574079257
6	1.3356419685657124	3.7493485682392613
7	0.9372577239790573	3.0695278521783287
8	0.6592821238727321	2.467605885792293
9	0.48635746561934595	2.0479182037053385
>10	2.24850456897514	17.647375763528565
>50	0.08493944777432988	2.7152840879133806
>100	0.027480188162029517	2.189847759683925
>500	9.047551423482738E-4	0.2962476879688666
>1k	4.5237757004919416E-4	0.4268953968650243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.0143352742638165E-5	0.0	0.0	0.0	0.0
3	3.0143352742638165E-5	0.0	0.0	0.0	0.0
4	3.0143352742638165E-5	0.0	0.0	0.0	0.0
5	3.0143352742638165E-5	0.0	0.0	0.0	0.0
6	6.028670548527633E-5	0.0	0.0	0.0	0.0
7	1.2057341097055266E-4	0.0	0.0	0.0	0.0
8	1.2057341097055266E-4	0.0	0.0	0.0	0.0
9	1.2057341097055266E-4	0.0	0.0	0.0	0.0
10-11	1.2057341097055266E-4	0.0	0.0	0.0	0.0
12-13	2.4114682194110532E-4	0.0	0.0	0.0	0.0
14-15	2.712901746837435E-4	0.0	0.0	0.0	0.0
16-17	2.712901746837435E-4	0.0	0.0	0.0	0.0
18-19	3.0143352742638163E-4	0.0	0.0	0.0	0.0
20-21	3.4664855654033884E-4	0.0	0.0	3.0143352742638165E-5	0.0
22-23	3.6172023291165795E-4	0.0	0.0	2.1100346919846716E-4	0.0
24-25	5.124369966248487E-4	0.0	0.0	5.275086729961678E-4	0.0
26-27	5.124369966248487E-4	0.0	0.0	0.0010550173459923358	0.0
28-29	6.330104075954015E-4	0.0	0.0	0.003391127183546793	0.0
30-31	6.932971130806778E-4	0.0	0.0	0.012946570002963093	0.0
32-33	7.083687894519968E-4	0.0	0.0	0.03832727301226443	0.0
34-35	8.289422004225495E-4	0.0	0.0	0.08060332523381444	0.0
36-37	9.344439350217831E-4	0.0	0.0	0.15442439610053532	0.0
38-39	0.001085160698734974	0.0	0.0	0.2750731654529446	0.0
40-41	0.0011002323751062928	0.0	0.0	0.4145012435640174	0.0
42-43	0.0012358774624481646	0.0	0.0	0.5485939482396434	0.0
44-45	0.0013564508734187175	0.0	0.0	0.7151359721427191	0.0
46-47	0.0014920959607605893	0.0	0.0	0.9188447499774679	0.0
48-49	0.0015071676371319082	0.0	0.0	1.1329981995375407	0.0
50-51	0.0015071676371319082	0.0	0.0	1.36024893586429	0.0
52-53	0.0015071676371319082	0.0	0.0	1.587650388954752	0.0
54-55	0.0015674543426171846	0.0	0.0	1.8444265392929153	0.0
56-57	0.0017483144590730135	0.0	0.0	2.1493868389901856	0.0
58-59	0.0017483144590730135	0.0	0.0	2.4749350486106776	0.0
60-61	0.0017633861354443324	0.0	0.0	2.8126461010628248	0.0
62-63	0.0017784578118156516	0.0	0.0	3.1576367732023183	0.0
64-65	0.00180860116455829	0.0	0.0	3.521014890514821	0.0
66-67	0.0018990312227862044	0.0	0.0	3.9438055560830643	0.0
68-69	0.0019291745755288425	0.0	0.0	4.42112554676274	0.0
70-71	0.0019894612810141188	0.0	0.0	4.934934065937378	0.0
72-73	0.0020648196628707143	0.0	0.0	5.443603143469398	0.0
74-75	0.0021100346919846716	0.0	0.0	5.950840411746141	0.0
76-77	0.0021100346919846716	0.0	0.0	6.5153801935866404	0.0
78-79	0.0021100346919846716	0.0	0.0	7.15316832259175	0.0
80-81	0.002381324866668415	0.0	0.0	7.829796161605749	0.0
82-83	0.0024416115721536914	0.0	0.0	8.513929695452664	0.0
84-85	0.002547113306752925	0.0	0.0	9.19554625934557	0.0
86-87	0.0026676867177234777	0.0	0.0	9.916831475447786	0.0
88-89	0.0026827583940947967	0.0	0.0	10.70649688724668	0.0
90-91	0.002743045099580073	0.0	0.0	11.539418010231257	0.0
92-93	0.002803331805065349	0.0	0.0	12.391208872032728	0.0
94-95	0.002803331805065349	0.0	0.0	13.24865161247344	0.0
96-97	0.0028334751578079874	0.0	0.0	14.117654328690955	0.0
98-99	0.0029841919215211786	0.0	0.0	15.03954355729543	0.0
100-101	0.003104765332491731	0.0	0.0	16.044040644091105	0.0
102-103	0.0031951953907196455	0.0	0.0	17.064619209574975	0.0
104-105	0.0033308404780615173	0.0	0.0	18.05573264775292	0.0
106-107	0.0034514138890320697	0.0	0.0	19.04877526050639	0.0
108-109	0.003496628918146027	0.0	0.0	20.08183317402571	0.0
110-111	0.0035870589763739415	0.0	0.0	21.178403131773777	0.0
112-113	0.0036473456818592177	0.0	0.0	22.309637945175872	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	14705	0.0	28.08143	1
GTATCAA	9705	0.0	27.997568	1
TACATGG	14870	0.0	27.118032	2
CATGGGG	8755	0.0	27.075792	4
ACATGGG	15135	0.0	26.013544	3
AACGCAG	10550	0.0	24.882345	6
TCAACGC	10730	0.0	24.645493	4
CAACGCA	10815	0.0	24.451794	5
TATCAAC	11165	0.0	23.953453	2
ATCAACG	11140	0.0	23.846804	3
ACGCAGA	12075	0.0	21.785534	7
CGCAGAG	12160	0.0	21.533413	8
GTACTAG	665	0.0	21.505058	1
GGTATCA	7400	0.0	20.935947	1
ATGGGGG	4765	0.0	18.499205	5
GCAGAGT	13605	0.0	18.458979	9
ATACCGT	390	5.86806E-9	18.315628	6
GTATAGG	790	0.0	18.102358	1
ATGGGGA	4385	0.0	17.521624	5
TAGACAG	1760	0.0	17.258846	5
>>END_MODULE