FastQCFastQC Report
Mon 15 Aug 2016
SRR2920917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3317481
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79990.24111667858836267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66410.20018200556386007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46020.13871970932162084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122100.037.4882471
GGTATCA83700.036.9334371
TCAACGC148850.030.1274724
CAACGCA150100.029.8368995
ATCAACG149850.029.8071923
TATCAAC151950.029.6704962
AACGCAG156300.028.5611956
GTACATG148900.027.8150731
TACATGG150900.026.837952
CATGGGG85950.026.0530454
ACATGGG149550.025.725433
ACGCAGA179650.024.6084947
CGCAGAG182900.024.167948
GAGTACT103950.022.64043412-13
AGTACTT110700.022.30813212-13
GCAGAGT197900.021.976699
GTATCGC2505.266065E-721.4277526
GTACTTT114800.020.57838814-15
AGAGTAC182700.019.80270410-11
CAGAGTA190500.019.4135810-11
Produced by FastQC (version 0.11.5)