##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920917_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3317481 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.094175369806187 31.0 31.0 34.0 28.0 34.0 2 31.2076406767665 33.0 31.0 34.0 28.0 34.0 3 31.283542241839516 33.0 31.0 34.0 27.0 34.0 4 34.82577624408399 37.0 35.0 37.0 32.0 37.0 5 34.519571626785506 37.0 35.0 37.0 31.0 37.0 6 34.234455299065765 37.0 35.0 37.0 30.0 37.0 7 34.131448228339515 37.0 35.0 37.0 30.0 37.0 8 34.12756516163921 37.0 35.0 37.0 30.0 37.0 9 35.67452835449547 39.0 35.0 39.0 30.0 39.0 10-11 35.63901104482588 39.0 35.0 39.0 30.0 39.0 12-13 35.5349540811236 39.0 35.0 39.0 30.0 39.0 14-15 36.74631535191912 40.0 37.0 41.0 30.0 41.0 16-17 36.75445842794578 40.0 37.0 41.0 30.0 41.0 18-19 36.7942001777855 40.0 37.0 41.0 30.0 41.0 20-21 36.792985249953205 40.0 37.0 41.0 30.0 41.0 22-23 36.7463947796536 40.0 37.0 41.0 30.0 41.0 24-25 36.723459757569074 40.0 37.0 41.0 30.0 41.0 26-27 36.59113631698267 40.0 36.5 41.0 30.0 41.0 28-29 36.49059768541252 40.0 36.0 41.0 29.5 41.0 30-31 36.33645256747514 40.0 36.0 41.0 29.0 41.0 32-33 36.17407439560317 40.0 36.0 41.0 28.5 41.0 34-35 36.04700268064836 40.0 35.0 41.0 28.0 41.0 36-37 35.913508924391735 40.0 35.0 41.0 27.0 41.0 38-39 35.78819954055501 40.0 35.0 41.0 27.0 41.0 40-41 35.64848012693969 40.0 35.0 41.0 26.5 41.0 42-43 35.49340026363376 40.0 35.0 41.0 26.0 41.0 44-45 35.29043271084296 39.5 35.0 41.0 24.5 41.0 46-47 35.059959800824785 39.0 35.0 41.0 24.0 41.0 48-49 34.78970098095513 39.0 34.5 41.0 23.0 41.0 50-51 34.597690235452745 39.0 34.0 41.0 22.5 41.0 52-53 34.396767909145524 39.0 34.0 41.0 21.5 41.0 54-55 34.1954950156459 38.5 34.0 41.0 20.5 41.0 56-57 33.95066814248521 38.0 34.0 41.0 19.5 41.0 58-59 33.71958739175899 38.0 33.0 40.0 18.5 41.0 60-61 33.45971371049299 37.5 33.0 40.0 18.0 41.0 62-63 33.16456205777817 37.0 33.0 40.0 16.0 41.0 64-65 32.8583328736472 36.5 32.5 40.0 13.0 41.0 66-67 32.53584798224918 36.0 32.0 39.0 12.0 41.0 68-69 32.17448977100396 35.5 32.0 39.0 8.0 41.0 70-71 31.842712738972732 35.0 32.0 38.5 7.5 40.0 72-73 31.475929025667366 35.0 31.0 37.5 7.0 40.0 74-75 31.143372185100684 35.0 31.0 37.0 5.5 39.0 76-77 30.929067868060134 35.0 31.0 36.5 2.0 39.0 78-79 30.773920935794358 35.0 31.0 36.0 2.0 39.0 80-81 30.518636429266664 35.0 31.0 36.0 2.0 37.0 82-83 30.289347700860983 35.0 31.0 35.0 2.0 37.0 84-85 30.047797259426655 35.0 31.0 35.0 2.0 36.5 86-87 29.880657492838694 35.0 31.0 35.0 2.0 36.0 88-89 29.723334361221664 35.0 31.0 35.0 2.0 36.0 90-91 29.56053779961362 35.0 31.0 35.0 2.0 36.0 92-93 29.434666242248262 35.0 30.0 35.0 2.0 35.0 94-95 29.340084992197394 34.5 30.0 35.0 2.0 35.0 96-97 29.27248264571824 34.0 30.0 35.0 2.0 35.0 98-99 29.168413926108393 34.0 30.0 35.0 2.0 35.0 100-101 29.062273604581307 34.0 30.0 35.0 2.0 35.0 102-103 27.65676261597278 32.5 27.0 34.5 2.0 35.0 104-105 28.721947616278737 34.0 29.0 35.0 2.0 35.0 106-107 28.89475810110141 34.0 30.0 35.0 2.0 35.0 108-109 28.99022888149171 34.5 30.0 35.0 2.0 35.0 110-111 29.159871450657896 35.0 31.0 35.0 2.0 35.0 112-113 29.128094931063657 35.0 31.0 35.0 2.0 35.0 114-115 29.04033271027023 35.0 31.0 35.0 2.0 35.0 116-117 28.91984701645616 35.0 31.0 35.0 2.0 35.0 118-119 28.81262439784885 35.0 30.5 35.0 2.0 35.0 120-121 28.623283147665354 35.0 30.0 35.0 2.0 35.0 122-123 28.42063556656391 35.0 30.0 35.0 2.0 35.0 124-125 27.670648000696914 34.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65148.0 3 25921.0 4 10948.0 5 9000.0 6 10660.0 7 12687.0 8 13948.0 9 15489.0 10 17549.0 11 19460.0 12 20945.0 13 23637.0 14 29426.0 15 27256.0 16 17042.0 17 14785.0 18 14015.0 19 13203.0 20 12753.0 21 13096.0 22 13493.0 23 15869.0 24 20837.0 25 28195.0 26 34837.0 27 41304.0 28 50084.0 29 59683.0 30 71592.0 31 87271.0 32 108538.0 33 139094.0 34 186576.0 35 276303.0 36 514388.0 37 913656.0 38 367806.0 39 987.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44358117701077 18.51762012070976 12.543665154270457 25.495133548009015 2 16.31978777282045 20.690703002532256 37.91378270830821 25.07572651633908 3 19.124240949037898 24.330143648840306 29.761670432091975 26.78394497002983 4 13.295898552306104 16.28940494011299 35.982364576745624 34.43233193083528 5 13.58117207524219 37.608093072872165 34.48431488963223 14.326419962253404 6 32.92881432610745 36.166213006597545 16.6595739868049 14.245398680490103 7 28.034914405493872 32.362972808035295 21.230832595622946 18.371280190847887 8 26.515763847539514 34.8744997248958 19.837620687835873 18.772115739728815 9 26.61300169219225 14.952853467848499 19.381736322501155 39.0524085174581 10-11 25.344423458690134 25.76889509249894 27.679801270098718 21.206880178712204 12-13 25.97202663963029 23.210294924982204 27.843710591426685 22.973967843960814 14-15 23.182587074201603 24.53506884516174 25.510329450069335 26.77201463056732 16-17 22.878630477855662 27.442181676418194 26.355576641021894 23.32361120470425 18-19 22.940020547671576 26.347210423684547 28.342475263271755 22.370293765372125 20-21 23.668676872761278 25.555359254872084 28.241385724712813 22.53457814765382 22-23 23.332384183495737 25.4773321764659 27.980495046206617 23.20978859383175 24-25 23.002387510481817 25.67025222837075 28.226285768378474 23.10107449276896 26-27 22.931014783213996 25.75375941379644 28.224687849800112 23.090537953189454 28-29 22.884550190074123 25.875133083539005 28.331256403573658 22.90906032281322 30-31 23.22218788860836 25.75796811998342 28.10706560651166 22.91277838489656 32-33 22.88251496885035 25.817767804155086 28.14183058250576 23.157886644488805 34-35 22.9546962671837 25.91516000432937 28.226414047663482 22.90372968082345 36-37 23.327415570885695 25.62133867655476 28.058424701384645 22.992821051174904 38-39 23.152722058372163 25.79406811191063 28.023998422536977 23.02921140718023 40-41 23.15322741610732 25.80990678021804 28.019150487324968 23.017715316349673 42-43 23.211163838516953 25.695753773014474 27.901817580461806 23.191264808006768 44-45 23.088527099974208 25.854881203248713 27.826257103753427 23.23033459302365 46-47 23.2001413403698 25.883121041384268 27.560226434025886 23.356511184220047 48-49 23.242137182996636 26.016763135874076 27.364754301619165 23.37634537951012 50-51 23.088399024290535 26.24569222944669 27.20067495314249 23.46523379312029 52-53 23.178839292160948 26.167319881937388 27.094588616071462 23.559252209830202 54-55 23.033960912570638 26.193006543514493 27.026216266410042 23.746816277504827 56-57 23.017248072094727 26.291796405966988 26.92544427937309 23.765511242565193 58-59 22.864438415371502 26.04322843289092 27.23334894031791 23.85898421141967 60-61 23.033736236187572 26.122627969179373 27.265897040359178 23.57773875427388 62-63 22.79293110809331 26.12004223582109 27.338015615916945 23.749011040168654 64-65 22.681147448065275 26.0633563832738 27.26274520208294 23.99275096657799 66-67 22.706855009701517 26.212063949960644 27.138905123826806 23.94217591651103 68-69 22.85426543624227 25.97052390013321 27.11627233201742 24.058938331607102 70-71 22.759616309354726 25.880095015199235 27.129845161644322 24.230443513801713 72-73 22.834113404515865 25.89429293018374 27.010482908458734 24.26111075684166 74-75 22.705612282659505 26.011278559868 26.953515094883134 24.32959406258936 76-77 22.735376880968044 25.802492099888998 27.0387743055409 24.42335671360206 78-79 22.794310063721305 25.886930861660613 26.985758577775204 24.333000496842878 80-81 22.702559782037675 25.932454772953147 26.837621293474474 24.527364151534705 82-83 22.6375581633196 25.83694207567212 26.915110532352365 24.61038922865591 84-85 22.634935350269263 25.833648008501882 26.739972790961474 24.79144385026738 86-87 22.59087919570429 25.738768204367375 26.86024059589564 24.810112004032696 88-89 22.51209464127459 25.642976048291434 26.83443369582963 25.01049561460435 90-91 22.539805570013772 25.61345927131489 26.796143959527647 25.05059119914369 92-93 22.483208021561264 25.61189728253333 26.763534575799053 25.14136012010636 94-95 22.278779130500844 25.524018145240913 26.90455237890234 25.2926503453559 96-97 22.47406854478154 25.289356632199922 26.888807222399425 25.347767600619107 98-99 22.457115061314372 25.366753445725553 26.634749699619647 25.541381793340427 100-101 22.26974817759107 25.3729309376433 26.792297991408244 25.565022893357387 102-103 22.23764070918742 25.24538132564784 26.765314163020122 25.751663802144613 104-105 22.184178317989158 25.344158662976827 26.59817558075738 25.873487438276626 106-107 22.148752034272086 25.389690187728064 26.623630611007385 25.83792716699247 108-109 22.111571501184507 25.334399157791665 26.6076001343385 25.94642920668533 110-111 22.042359597594224 25.482503614980494 26.531477274046196 25.94365951337908 112-113 21.952514327729457 25.552918768986444 26.47625128535809 26.018315617926007 114-115 21.930762239374907 25.670409207585166 26.322331800444044 26.07649675259589 116-117 21.8517363532625 25.54213231954935 26.444737885664736 26.161393441523412 118-119 21.84454934901707 25.460612077537075 26.410224372563324 26.28461420088253 120-121 21.798058958326926 25.638377593978888 26.29340982711128 26.270153620582903 122-123 21.687593559094985 25.761329128461803 26.16991446972981 26.381162842713408 124-125 21.592152681663965 25.77667372175347 26.141319673503666 26.489853923078904 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 178.0 1 150.0 2 285.5 3 495.5 4 375.0 5 248.5 6 438.0 7 1029.0 8 1716.0 9 1885.0 10 1536.0 11 1232.0 12 1275.0 13 1388.0 14 1501.5 15 1749.5 16 2003.5 17 2233.0 18 2500.0 19 2805.0 20 3269.0 21 3899.0 22 4731.0 23 5851.0 24 7152.0 25 8971.5 26 11946.5 27 15671.0 28 18916.5 29 22991.0 30 28267.0 31 33959.0 32 40004.0 33 45571.0 34 51028.0 35 57212.5 36 64577.5 37 70878.0 38 76369.5 39 82060.5 40 87476.5 41 92671.0 42 97585.0 43 105120.0 44 112971.0 45 118697.5 46 123508.5 47 127215.0 48 131781.0 49 136241.5 50 141005.5 51 140976.5 52 135749.5 53 133739.5 54 130288.0 55 122783.0 56 114098.0 57 103284.0 58 93312.0 59 82952.5 60 69588.5 61 56841.0 62 45974.0 63 35321.5 64 25587.5 65 18903.5 66 14237.5 67 10932.5 68 8191.5 69 5803.0 70 4322.5 71 3219.0 72 2410.0 73 1937.0 74 1482.0 75 1077.0 76 722.5 77 439.5 78 259.5 79 144.5 80 82.5 81 67.5 82 47.5 83 27.0 84 23.5 85 15.0 86 9.5 87 10.0 88 6.5 89 6.0 90 10.0 91 8.5 92 4.5 93 8.5 94 12.0 95 10.5 96 8.5 97 6.0 98 4.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21284221371576806 2 0.008470282120681325 3 4.822936438822106E-4 4 0.002019604633756757 5 0.08274350327854176 6 0.05594606269033643 7 0.027520881054028647 8 0.01675970412490682 9 0.014529096021951595 10-11 0.012539634740937475 12-13 0.014107089083554661 14-15 0.021899145767526627 16-17 0.02473262092533461 18-19 0.024220183928709765 20-21 0.017302284474274305 22-23 0.014393450934609722 24-25 0.012886283297477815 26-27 0.012298487918996369 28-29 0.014634597756550828 30-31 0.010731033576379187 32-33 0.015358038222374146 34-35 0.018146298351068173 36-37 0.019005383904233362 38-39 0.02299937814263292 40-41 0.024205112252338446 42-43 0.022200579294953008 44-45 0.019909684486512508 46-47 0.02004532957385438 48-49 0.01867380702406434 50-51 0.015373109898745465 52-53 0.012886283297477815 54-55 0.018357301820266642 56-57 0.022110149236725093 58-59 0.017784578118156518 60-61 0.020391978130394717 62-63 0.02607400012238201 64-65 0.018839595464148852 66-67 0.02204986253123982 68-69 0.01574990180802844 70-71 0.016217123775539334 72-73 0.0240091804595113 74-75 0.014453737640094999 76-77 0.013232931854018155 78-79 0.016186980422796694 80-81 0.01740778620887354 82-83 0.012675279828279349 84-85 0.017136496034189794 86-87 0.017829793147270474 88-89 0.01867380702406434 90-91 0.013609723763301131 92-93 0.012690351504650667 94-95 0.014107089083554661 96-97 0.014076945730812022 98-99 0.013157573472161559 100-101 0.017483144590730137 102-103 0.022773302997063132 104-105 0.00786741506582856 106-107 0.007912630094942518 108-109 0.014679812785664787 110-111 0.01149968907131646 112-113 0.009404726055703107 114-115 0.006510964192409843 116-117 0.004235141060340663 118-119 0.0022456797793265434 120-121 0.0026224716886095205 122-123 0.0017784578118156518 124-125 4.973653202535298E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3317481.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.260352519213406 #Duplication Level Percentage of deduplicated Percentage of total 1 66.99333137288819 30.32141794373398 2 14.565494214172963 13.184788055000633 3 6.607868126826329 8.97223322461902 4 3.6432185667671058 6.595734265456904 5 2.172713002634562 4.916887821115944 6 1.4027033005376988 3.8092107517320226 7 0.9536168676183547 3.021272491767236 8 0.700954335315283 2.5380352252992497 9 0.5097826005209053 2.076564618696376 >10 2.3206774450605874 17.660186889017346 >50 0.09465967497291121 2.9087985673928913 >100 0.03264724594425165 2.5279460429679896 >500 0.0016666048161645653 0.5025523035700358 >1k 5.333135398494422E-4 0.5226471532819599 >5k 1.3332838496236054E-4 0.4417246463484523 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7999 0.24111667858836267 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6641 0.20018200556386007 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4602 0.13871970932162084 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.043005822791449E-5 0.0 0.0 0.0 0.0 7 1.2057341097055266E-4 0.0 0.0 0.0 0.0 8 1.2057341097055266E-4 0.0 0.0 0.0 0.0 9 1.2057341097055266E-4 0.0 0.0 0.0 0.0 10-11 1.5071676371319082E-4 0.0 0.0 0.0 0.0 12-13 2.8636185105506253E-4 0.0 0.0 0.0 0.0 14-15 3.6172023291165795E-4 0.0 0.0 0.0 0.0 16-17 3.7679190928297706E-4 0.0 0.0 0.0 0.0 18-19 4.8229364388221064E-4 0.0 0.0 3.0143352742638165E-5 0.0 20-21 6.028670548527633E-4 0.0 0.0 6.028670548527633E-5 0.0 22-23 6.028670548527633E-4 0.0 0.0 2.4114682194110532E-4 0.0 24-25 7.535838185659541E-4 0.0 0.0 5.576520257388061E-4 0.0 26-27 7.686554949372733E-4 0.0 0.0 0.001085160698734974 0.0 28-29 9.796589641357404E-4 0.0 0.0 0.003466485565403389 0.0 30-31 0.0011755907569628884 0.0 0.0 0.01299178503207705 0.0 32-33 0.0012208057860768457 1.5071676371319082E-5 0.0 0.03805598283758068 0.0 34-35 0.0013112358443047602 3.0143352742638165E-5 0.0 0.08019638997178884 0.0 36-37 0.0014468809316466318 3.0143352742638165E-5 0.0 0.15294737181614604 0.0 38-39 0.0014770242843892701 3.0143352742638165E-5 0.0 0.2719684001204528 0.0 40-41 0.0014770242843892701 3.0143352742638165E-5 0.0 0.40957280539059604 0.0 42-43 0.0015373109898745465 3.0143352742638165E-5 0.0 0.5420076256653767 0.0 44-45 0.0015975976953598228 3.0143352742638165E-5 0.0 0.706816406785751 0.0 46-47 0.0018839595464148853 3.0143352742638165E-5 0.0 0.907993142990118 0.0 48-49 0.002019604633756757 3.0143352742638165E-5 0.0 1.1197200526544084 0.0 50-51 0.002019604633756757 3.0143352742638165E-5 0.0 1.344860754289173 0.0 52-53 0.002019604633756757 3.0143352742638165E-5 0.0 1.5700014559239375 0.0 54-55 0.002019604633756757 3.0143352742638165E-5 0.0 1.8237331276350943 0.0 56-57 0.0021100346919846716 3.0143352742638165E-5 0.0 2.126553249287637 0.0 58-59 0.0021100346919846716 3.0143352742638165E-5 0.0 2.4501119976271153 0.0 60-61 0.0021100346919846716 3.0143352742638165E-5 0.0 2.785984305561961 0.0 62-63 0.0021100346919846716 3.0143352742638165E-5 0.0 3.1291362331841537 0.0 64-65 0.0021100346919846716 3.0143352742638165E-5 0.0 3.4906906776557274 0.0 66-67 0.002170321397469948 3.0143352742638165E-5 0.0 3.9106478680661625 0.0 68-69 0.002200464750212586 3.0143352742638165E-5 0.0 4.38423008300575 0.0 70-71 0.0022607514556978623 3.0143352742638165E-5 0.0 4.891452279606122 0.0 72-73 0.0022908948084405007 3.0143352742638165E-5 0.0 5.393896754796787 0.0 74-75 0.0023210381611831385 3.0143352742638165E-5 0.0 5.895708219579856 0.0 76-77 0.0023210381611831385 3.0143352742638165E-5 0.0 6.455997788683643 0.0 78-79 0.0023210381611831385 3.0143352742638165E-5 0.0 7.08849575928242 0.0 80-81 0.002351181513925777 3.0143352742638165E-5 0.0 7.759652579773629 0.0 82-83 0.002381324866668415 3.0143352742638165E-5 0.0 8.438164378334044 0.0 84-85 0.0024114682194110535 3.0143352742638165E-5 0.0 9.113933131794877 0.0 86-87 0.0024566832485250103 3.0143352742638165E-5 0.0 9.83003369122536 0.0 88-89 0.0024717549248963293 3.0143352742638165E-5 0.0 10.612886705304415 0.0 90-91 0.002562184983124244 3.0143352742638165E-5 0.0 11.438271990103335 0.0 92-93 0.0026224716886095205 3.0143352742638165E-5 0.0 12.283988966327161 0.0 94-95 0.0026224716886095205 3.0143352742638165E-5 0.0 13.137030777267451 0.0 96-97 0.0026224716886095205 3.0143352742638165E-5 0.0 14.000652905020406 0.0 98-99 0.0026978300704661156 3.0143352742638165E-5 0.0 14.91579002261053 0.0 100-101 0.00278826012869403 3.0143352742638165E-5 0.0 15.916338330196918 0.0 102-103 0.0028786901869219447 3.0143352742638165E-5 0.0 16.933148976587958 0.0 104-105 0.0031951953907196455 3.0143352742638165E-5 0.0 17.91977105520725 0.0 106-107 0.0033459121544328362 3.0143352742638165E-5 1.5071676371319082E-5 18.913115101488145 0.0 108-109 0.0033459121544328362 3.0143352742638165E-5 3.0143352742638165E-5 19.949971077453043 0.0 110-111 0.0034061988599181124 3.0143352742638165E-5 3.0143352742638165E-5 21.04851542480575 0.0 112-113 0.0034061988599181124 3.0143352742638165E-5 3.0143352742638165E-5 22.186185844018397 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12210 0.0 37.488247 1 GGTATCA 8370 0.0 36.933437 1 TCAACGC 14885 0.0 30.127472 4 CAACGCA 15010 0.0 29.836899 5 ATCAACG 14985 0.0 29.807192 3 TATCAAC 15195 0.0 29.670496 2 AACGCAG 15630 0.0 28.561195 6 GTACATG 14890 0.0 27.815073 1 TACATGG 15090 0.0 26.83795 2 CATGGGG 8595 0.0 26.053045 4 ACATGGG 14955 0.0 25.72543 3 ACGCAGA 17965 0.0 24.608494 7 CGCAGAG 18290 0.0 24.16794 8 GAGTACT 10395 0.0 22.640434 12-13 AGTACTT 11070 0.0 22.308132 12-13 GCAGAGT 19790 0.0 21.97669 9 GTATCGC 250 5.266065E-7 21.427752 6 GTACTTT 11480 0.0 20.578388 14-15 AGAGTAC 18270 0.0 19.802704 10-11 CAGAGTA 19050 0.0 19.41358 10-11 >>END_MODULE