FastQCFastQC Report
Mon 15 Aug 2016
SRR2920916_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920916_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512144
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42050.16738690138781853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG66200.028.4300734
GTACATG114450.028.3190861
TACATGG116350.027.1310772
GTATCGC1807.272083E-726.4544056
ACATGGG117900.025.8145543
GTATCAA90700.024.0419641
ATGGGGA30250.023.2330115
CTATGCG2403.5583253E-722.317799
AACGCAG98400.021.9579626
TCAACGC101250.021.0589584
CAACGCA102050.020.9522325
TATCAAC104650.020.7735712
TATACCG1754.7535964E-420.4202985
ATCAACG105300.020.3059643
ACGCAGA106400.019.8015377
CGCAGAG107750.019.6631328
GGTATCA72350.018.6108421
ATGGGGG34950.018.0637365
GAGTACT32450.017.69305212-13
GTATAGG6100.017.5808051
Produced by FastQC (version 0.11.5)