##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920916_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2512144
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.659927934067476	31.0	30.0	34.0	16.0	34.0
2	28.69376437019534	31.0	30.0	34.0	16.0	34.0
3	28.794779678235006	31.0	30.0	34.0	16.0	34.0
4	32.12311953454898	35.0	33.0	37.0	18.0	37.0
5	32.228820879694794	35.0	33.0	37.0	19.0	37.0
6	32.23153330382335	37.0	33.0	37.0	19.0	37.0
7	32.09244334719666	36.0	33.0	37.0	17.0	37.0
8	31.98597652045424	36.0	33.0	37.0	17.0	37.0
9	33.264453789273226	38.0	33.0	39.0	17.0	39.0
10-11	33.28540203109375	38.0	33.0	39.0	17.0	39.0
12-13	33.21655307179843	38.0	33.0	39.0	16.5	39.0
14-15	34.24326929507225	39.0	33.0	41.0	16.5	41.0
16-17	34.2224751049303	39.0	33.0	41.0	17.0	41.0
18-19	34.20286237572368	39.0	33.5	41.0	16.5	41.0
20-21	34.24826741619907	39.0	34.0	41.0	17.0	41.0
22-23	34.16107615646237	39.0	33.0	41.0	17.0	41.0
24-25	34.16777461801553	39.0	33.0	41.0	17.0	41.0
26-27	34.08745895935902	39.0	33.0	41.0	16.5	41.0
28-29	33.95985918800833	39.0	33.0	41.0	16.0	41.0
30-31	33.78823526836041	39.0	33.0	41.0	13.0	41.0
32-33	33.63603181187065	39.0	33.0	41.0	9.5	41.0
34-35	33.54971649714348	39.0	33.0	41.0	9.0	41.0
36-37	33.37981540867084	38.0	32.0	41.0	8.0	41.0
38-39	33.21732631568891	38.0	32.0	41.0	7.5	41.0
40-41	33.07140633657943	38.0	32.0	41.0	2.0	41.0
42-43	32.850406266519755	38.0	31.5	40.5	2.0	41.0
44-45	32.66540711838175	38.0	31.0	40.0	2.0	41.0
46-47	32.442862749906055	38.0	31.0	40.0	2.0	41.0
48-49	32.29776816137928	38.0	31.0	40.0	2.0	41.0
50-51	32.08190872020076	38.0	31.0	40.0	2.0	41.0
52-53	31.874872220700723	37.5	30.0	40.0	2.0	41.0
54-55	31.627233152239683	37.0	30.0	40.0	2.0	41.0
56-57	31.348751106624462	37.0	30.0	40.0	2.0	41.0
58-59	31.072098374933923	36.0	29.5	40.0	2.0	41.0
60-61	30.800583485660056	36.0	29.0	40.0	2.0	41.0
62-63	30.488755421663726	35.5	29.0	39.0	2.0	41.0
64-65	30.206870505830878	35.0	29.0	39.0	2.0	40.5
66-67	29.86054143393054	35.0	28.0	39.0	2.0	40.0
68-69	29.536250708558107	35.0	28.0	38.0	2.0	40.0
70-71	29.198483247775606	34.5	28.0	37.5	2.0	40.0
72-73	29.257533803794686	35.0	28.5	37.0	2.0	39.5
74-75	29.26567426071117	35.0	29.0	37.0	2.0	39.0
76-77	29.006102556222892	35.0	29.0	36.0	2.0	39.0
78-79	28.717674623747683	34.5	29.0	36.0	2.0	39.0
80-81	28.45286894381851	34.0	28.5	35.5	2.0	37.0
82-83	28.20957337636696	34.0	28.0	35.0	2.0	37.0
84-85	27.9710100217185	34.0	28.0	35.0	2.0	36.5
86-87	27.770337608035206	34.0	27.0	35.0	2.0	36.0
88-89	27.560310236992784	34.0	27.0	35.0	2.0	36.0
90-91	27.377121096561346	34.0	26.5	35.0	2.0	35.5
92-93	27.204013185549872	34.0	26.0	35.0	2.0	35.0
94-95	27.076069285837118	34.0	26.0	35.0	2.0	35.0
96-97	26.97347425147603	34.0	25.5	35.0	2.0	35.0
98-99	26.833597914769218	34.0	25.0	35.0	2.0	35.0
100-101	26.695433462412982	34.0	25.0	35.0	2.0	35.0
102-103	25.354349113745073	31.5	21.5	34.0	2.0	35.0
104-105	26.346542833531835	33.5	24.5	35.0	2.0	35.0
106-107	26.50406425746295	34.0	25.0	35.0	2.0	35.0
108-109	26.432880041908426	34.0	25.0	35.0	2.0	35.0
110-111	26.32595683209243	34.0	24.0	35.0	2.0	35.0
112-113	26.19731611722895	34.0	24.0	35.0	2.0	35.0
114-115	26.077226862791306	34.0	23.5	35.0	2.0	35.0
116-117	25.844581560611175	34.0	21.5	35.0	2.0	35.0
118-119	25.627957832035108	34.0	19.0	35.0	2.0	35.0
120-121	25.432593434134347	34.0	17.5	35.0	2.0	35.0
122-123	25.11668777745225	34.0	2.0	35.0	2.0	35.0
124-125	23.825773124470572	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	146178.0
3	20566.0
4	13575.0
5	13361.0
6	18079.0
7	18105.0
8	16528.0
9	17231.0
10	18394.0
11	19125.0
12	19230.0
13	18677.0
14	19064.0
15	17193.0
16	14599.0
17	13130.0
18	12611.0
19	12506.0
20	12704.0
21	13714.0
22	15679.0
23	19873.0
24	26253.0
25	32123.0
26	35836.0
27	39247.0
28	45512.0
29	52958.0
30	62038.0
31	74026.0
32	91544.0
33	115251.0
34	152369.0
35	219208.0
36	370784.0
37	529838.0
38	174751.0
39	284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73008790628646	19.142325565074817	12.171268528464873	24.95631800017385
2	15.781674576141452	21.964141056552744	37.96607696429255	24.288107403013253
3	18.917261331530895	25.627752754998074	29.391019314395773	26.063966599075254
4	12.699053076435185	17.335203287781763	36.10667829674152	33.859065339041535
5	13.173156751764711	39.045424348446076	33.91186765633586	13.869551243453357
6	31.559086885950805	37.34230459159295	17.10417092574283	13.994437596713407
7	26.94314476165959	33.90002203737746	21.24327471444062	17.91355848652233
8	25.46020581454377	36.19848759064056	20.09222047163774	18.249086123177925
9	25.788460473269552	16.87983840470472	19.490858461059048	37.84084266096668
10-11	24.389551504381764	27.465072405158107	27.584522488530123	20.560853601930006
12-13	24.660807035541236	25.379539761664066	27.413671697981663	22.54598150481303
14-15	22.52527856115876	26.689842083939048	24.860316055217837	25.924563299684362
16-17	22.31578452535634	29.1258914053594	25.922180394290944	22.636143674993324
18-19	22.451113750575406	28.3286779743123	27.328972573584583	21.891235701527716
20-21	23.532187340970406	27.42669575964236	26.918717266667798	22.12239963271944
22-23	23.05399084034169	27.318672940929044	26.69409465723775	22.933241561491517
24-25	22.762807572133962	27.44121813144003	26.87538737797096	22.920586918455044
26-27	22.581029400047807	27.40092422914509	26.96233367859135	23.05571269221576
28-29	22.616895324864206	27.77274413937431	26.99703047423779	22.613330061523698
30-31	22.897932543513424	27.5976750351089	26.806168809117736	22.69822361225994
32-33	22.445122658094668	27.784660836295593	26.770175472842883	23.000041032766855
34-35	22.789826544370186	27.765967257692846	26.83565724930121	22.608548948635757
36-37	22.95015582405088	27.478853303896138	26.794227399138638	22.77676347291434
38-39	22.692755770289104	27.662929588540308	26.79719451243669	22.8471201287339
40-41	22.758938977185288	27.58431049631294	26.92123597298303	22.735514553518733
42-43	22.615216561641247	27.6337689717017	26.739094234451148	23.011920232205902
44-45	22.581028730767315	27.749818254244886	26.767972912413484	22.90118010257432
46-47	22.566923713956914	27.76419446366424	26.750126372621775	22.918755449757075
48-49	22.460407220669836	27.77832544395314	26.657541552428484	23.10372578294854
50-51	22.3025257984201	27.931284675978457	26.707850132654258	23.058339392947186
52-53	22.411204257742224	27.738978668467677	26.55514166150655	23.294675412283553
54-55	22.362434383019135	27.729662420600366	26.53967372556268	23.368229470817823
56-57	22.493002867370375	27.645824386909368	26.49664543957449	23.364527306145767
58-59	22.356991486394744	27.41777606682948	26.638628534647797	23.58660391212798
60-61	22.351995216634702	27.28593613721343	26.812339951158528	23.54972869499334
62-63	22.218754574523278	27.238516515087092	26.844114188067554	23.69861472232208
64-65	22.158188505675707	27.26334725598663	26.78964645302932	23.788817785308343
66-67	22.270279593123554	27.28274503383932	26.60070080512527	23.846274567911856
68-69	22.445790897618924	27.041773950950898	26.753918797481262	23.75851635394891
70-71	22.176006952936238	26.894491901119817	27.00994266695451	23.919558478989433
72-73	22.197142955009465	26.85003739983893	27.04789011536994	23.90492952978166
74-75	22.043930609130776	27.05420994866404	27.02750338213472	23.874356060070458
76-77	22.051967612210134	26.708055977745293	27.24621926349705	23.99375714654752
78-79	22.16639112926916	26.84260033215849	27.156276805646257	23.83473173292609
80-81	22.156122234988143	26.738789141439035	27.198760610302635	23.906328013270183
82-83	22.119645597795802	26.577078257114874	27.357924636752877	23.94535150833645
84-85	22.16707688741044	26.660338419167655	27.06234014237362	24.110244551048286
86-87	22.0632386302645	26.564191887880256	27.274101445410594	24.098468036444654
88-89	22.191968517736655	26.40702296643857	27.14413172841768	24.256876787407094
90-91	22.163899165019444	26.37080704829145	27.23181788760375	24.233475899085356
92-93	22.31355829928892	26.308812992120494	27.177498146924496	24.200130561666093
94-95	22.00620680836927	26.29199962238132	27.373849268801965	24.327944300447445
96-97	22.03828716027369	26.16091472423315	27.386485406283562	24.414312709209593
98-99	21.904712783807927	26.329979699770075	27.21804008190189	24.54726743452011
100-101	21.83497689730727	26.495760706165967	27.308073805152787	24.361188591373974
102-103	21.704021176306128	26.530739038029694	27.273893089762534	24.49134669590164
104-105	21.531563140817962	26.691679765953218	27.17653590213429	24.60022119109453
106-107	21.36266260877843	26.77925434511288	27.239789609567662	24.618293436541027
108-109	21.300154261058463	26.91749921037297	27.302992537078612	24.47935399148995
110-111	21.274679112534823	27.165521110647944	27.12861608959336	24.43118368722387
112-113	21.236702837445	27.316373086136696	27.08494489937853	24.361979177039775
114-115	21.205397110241623	27.380306263798747	27.049325460804823	24.3649711651548
116-117	21.144511673133927	27.2173417353925	27.113630560359027	24.524516031114548
118-119	21.14282061081853	27.22001627951858	26.99950388694916	24.637659222713733
120-121	21.182402874021182	27.378903596290684	27.034150112956056	24.404543416732075
122-123	21.22413781085164	27.321206472306287	26.91728978895996	24.53736592788212
124-125	21.163475148063117	27.408119599386037	26.81885487762758	24.609550374923263
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3393.0
1	2408.5
2	1169.5
3	846.0
4	680.5
5	570.5
6	554.0
7	557.5
8	623.0
9	675.5
10	671.0
11	693.0
12	770.0
13	862.5
14	981.5
15	1144.0
16	1330.5
17	1563.5
18	1851.5
19	2265.5
20	2790.0
21	3481.0
22	4441.5
23	5717.0
24	7525.0
25	9417.0
26	12208.5
27	16089.5
28	19167.0
29	22472.0
30	26359.0
31	30964.5
32	36646.0
33	41463.5
34	45151.0
35	49068.5
36	54515.0
37	58994.5
38	61848.5
39	65474.0
40	70175.0
41	74539.5
42	77589.5
43	82019.5
44	86605.0
45	90259.5
46	94109.5
47	96708.0
48	100210.0
49	103458.5
50	105224.5
51	104245.5
52	98372.5
53	93655.0
54	89982.5
55	83953.5
56	76231.5
57	67641.0
58	60914.0
59	54667.0
60	46770.0
61	38999.5
62	31942.0
63	23997.0
64	16871.0
65	12326.5
66	9133.5
67	6857.5
68	5075.0
69	3682.0
70	2683.0
71	1931.0
72	1405.0
73	1083.5
74	823.5
75	595.5
76	415.0
77	283.5
78	198.0
79	140.5
80	106.0
81	83.5
82	78.5
83	62.0
84	47.5
85	42.5
86	34.0
87	23.5
88	18.5
89	16.0
90	17.0
91	19.0
92	12.5
93	11.0
94	14.0
95	14.0
96	10.0
97	11.0
98	10.0
99	10.0
100	14.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1690189734346439
2	0.1496729486844703
3	0.17630358769242527
4	0.14748358374360704
5	0.17395499621040833
6	0.11133915890171901
7	0.11030418638422002
8	0.09975542803278793
9	0.10608468304364718
10-11	0.0919135208809686
12-13	0.08086717958843125
14-15	0.08013075683559541
16-17	0.07931472081218274
18-19	0.07780206867122268
20-21	0.07171165347209396
22-23	0.06249641740282404
24-25	0.06671592074339687
26-27	0.07738409900069422
28-29	0.07206991318968976
30-31	0.06928344871950017
32-33	0.07782197198886688
34-35	0.07585154354208994
36-37	0.07726467909482895
38-39	0.07362237196593825
40-41	0.07736419568305002
42-43	0.07794139189473215
44-45	0.07041793782522021
46-47	0.06810915297849168
48-49	0.05933178989739442
50-51	0.06649698424931055
52-53	0.06789021648440535
54-55	0.06934315867243279
56-57	0.07284614257781401
58-59	0.06032695577960499
60-61	0.07075629422517181
62-63	0.06514355864950416
64-65	0.05921236999152915
66-67	0.063810036367342
68-69	0.06687514728455057
70-71	0.06170028469705558
72-73	0.057241941544752206
74-75	0.06072502213248922
76-77	0.06980093497824966
78-79	0.06351148660267883
80-81	0.06183960792056506
82-83	0.06619843448464738
84-85	0.06794992643733798
86-87	0.061401734932392406
88-89	0.06066531217955659
90-91	0.06394935959085148
92-93	0.05783904107407856
94-95	0.06667611410810845
96-97	0.0539180874981689
98-99	0.05314185811004465
100-101	0.051330656204421406
102-103	0.048743224910673906
104-105	0.047489315899088586
106-107	0.05885411027393335
108-109	0.05831672069753963
110-111	0.06550181836709998
112-113	0.06552172168474418
114-115	0.06872615582546224
116-117	0.07193058996618028
118-119	0.06478529893190836
120-121	0.06586007808469578
122-123	0.06375032641440936
124-125	0.07551318714213834
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2512144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.5148084915744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88604030835137	35.59423519085616
2	12.605793763952677	12.483469282127992
3	5.703844315802864	8.47274276888202
4	3.0187421009664064	5.978897480592182
5	1.8436993529931014	4.564521018974652
6	1.1496634036723619	3.4155217957565154
7	0.816432199614325	2.82978388071807
8	0.5528434339350388	2.1899149405694245
9	0.40354224544481426	1.79831853013139
>10	1.9021510345494905	16.105574414506197
>50	0.08554285473750085	2.8700605654345566
>100	0.02920323828477256	2.4795228113615586
>500	0.0020175384638879547	0.6815043651874371
>1k	4.84209231330233E-4	0.5359329549019562
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4205	0.16738690138781853	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3883981173053773E-4
2	0.0	0.0	0.0	0.0	2.3883981173053773E-4
3	0.0	0.0	0.0	0.0	2.3883981173053773E-4
4	0.0	0.0	0.0	0.0	2.786464470189607E-4
5	0.0	0.0	0.0	0.0	3.1845308230738363E-4
6	0.0	0.0	0.0	0.0	5.174862587494984E-4
7	0.0	0.0	0.0	0.0	5.572928940379214E-4
8	0.0	0.0	0.0	0.0	5.970995293263444E-4
9	0.0	0.0	0.0	0.0	7.563260704800362E-4
10-11	0.0	0.0	0.0	3.582597175958066E-4	7.961327057684592E-4
12-13	0.0	0.0	0.0	3.582597175958066E-4	0.0011145857880758428
14-15	0.0	0.0	0.0	4.378729881726525E-4	0.001413135552739015
16-17	0.0	0.0	0.0	4.7767962346107547E-4	0.0017116853174021871
18-19	0.0	0.0	0.0	5.771962116821328E-4	0.0017514919526906102
20-21	0.0	0.0	0.0	6.767127999031902E-4	0.0018510085409116674
22-23	0.0	0.0	0.0	0.0010946824704316312	0.001950525129132725
24-25	0.0	0.0	0.0	0.0016519753644695526	0.0021097516702864166
26-27	0.0	0.0	0.0	0.0033437573642275285	0.002189364940863263
28-29	0.0	0.0	0.0	0.009115719481048857	0.0022092682585074745
30-31	0.0	0.0	0.0	0.027207835219637093	0.002249074893795897
32-33	0.0	0.0	0.0	0.07437869803641829	0.0023087848467285314
34-35	0.0	0.0	0.0	0.15042927475495035	0.0024680113878822233
36-37	0.0	0.0	0.0	0.2781289607602112	0.0024680113878822233
38-39	0.0	0.0	0.0	0.4781573030845365	0.002627237929035915
40-41	0.0	0.0	0.0	0.701154073970282	0.0027466578349011842
42-43	0.0	0.0	0.0	0.9034712978236916	0.0027466578349011842
44-45	3.9806635288422953E-5	0.0	0.0	1.1538749371055164	0.0029058843760548757
46-47	3.9806635288422953E-5	0.0	0.0	1.4544150335331096	0.003065110917208568
48-49	3.9806635288422953E-5	0.0	0.0	1.7627373271595896	0.0034233706348043742
50-51	3.9806635288422953E-5	0.0	0.0	2.0835987109019225	0.0035427905406696433
52-53	3.9806635288422953E-5	0.0	0.0	2.392418587469508	0.0037617270347559692
54-55	3.9806635288422953E-5	0.0	0.0	2.7359299466909537	0.003960760211198084
56-57	3.9806635288422953E-5	0.0	0.0	3.1319860644931183	0.004020470164130719
58-59	3.9806635288422953E-5	0.0	0.0	3.55775783553809	0.0041398900699959875
60-61	3.9806635288422953E-5	0.0	0.0	3.998297868275067	0.004219503340572833
62-63	3.9806635288422953E-5	0.0	0.0	4.42440799571999	0.004299116611149679
64-65	3.9806635288422953E-5	0.0	0.0	4.879079383984358	0.004319019928793891
66-67	3.9806635288422953E-5	0.0	0.0	5.399292397251113	0.004418536517014948
68-69	3.9806635288422953E-5	0.0	0.0	5.978319714156513	0.004518053105236005
70-71	3.9806635288422953E-5	0.0	0.0	6.606946098631289	0.004637473011101274
72-73	3.9806635288422953E-5	0.0	0.0	7.222794553178479	0.005095249316918139
74-75	3.9806635288422953E-5	0.0	0.0	7.831537523326688	0.00525447585807183
76-77	3.9806635288422953E-5	0.0	0.0	8.492646122196817	0.005294282493360253
78-79	3.9806635288422953E-5	0.0	0.0	9.230840270302977	0.005334089128648676
80-81	3.9806635288422953E-5	0.0	0.0	10.002073925698527	0.005473412352158157
82-83	3.9806635288422953E-5	0.0	0.0	10.796634269373094	0.005752058799177118
84-85	3.9806635288422953E-5	1.9903317644211477E-5	0.0	11.578197746625989	0.005871478705042387
86-87	3.9806635288422953E-5	3.9806635288422953E-5	0.0	12.396184295167792	0.005951091975619232
88-89	3.9806635288422953E-5	3.9806635288422953E-5	0.0	13.278677496194486	0.006010801928551866
90-91	3.9806635288422953E-5	3.9806635288422953E-5	0.0	14.213795069072473	0.006150125152061347
92-93	3.9806635288422953E-5	3.9806635288422953E-5	0.0	15.167641663853665	0.006309351693215039
94-95	3.9806635288422953E-5	3.9806635288422953E-5	0.0	16.099514995955644	0.0065083848696571535
96-97	3.9806635288422953E-5	3.9806635288422953E-5	0.0	17.06241760026495	0.006747224681387691
98-99	3.9806635288422953E-5	3.9806635288422953E-5	0.0	18.085925806800883	0.00686664458725296
100-101	3.9806635288422953E-5	3.9806635288422953E-5	0.0	19.179195141679777	0.0069661611754740175
102-103	3.9806635288422953E-5	3.9806635288422953E-5	0.0	20.28745167474476	0.0070855810813392865
104-105	3.9806635288422953E-5	3.9806635288422953E-5	0.0	21.367843563107847	0.0072050009872045555
106-107	3.9806635288422953E-5	3.9806635288422953E-5	0.0	22.444115464718585	0.007284614257781401
108-109	3.9806635288422953E-5	3.9806635288422953E-5	0.0	23.542957728537854	0.0075234540695119385
110-111	3.9806635288422953E-5	3.9806635288422953E-5	0.0	24.69868367418428	0.007722487245954053
112-113	3.9806635288422953E-5	3.9806635288422953E-5	0.0	25.889459362202167	0.007722487245954053
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	6620	0.0	28.430073	4
GTACATG	11445	0.0	28.319086	1
TACATGG	11635	0.0	27.131077	2
GTATCGC	180	7.272083E-7	26.454405	6
ACATGGG	11790	0.0	25.814554	3
GTATCAA	9070	0.0	24.041964	1
ATGGGGA	3025	0.0	23.233011	5
CTATGCG	240	3.5583253E-7	22.31779	9
AACGCAG	9840	0.0	21.957962	6
TCAACGC	10125	0.0	21.058958	4
CAACGCA	10205	0.0	20.952232	5
TATCAAC	10465	0.0	20.773571	2
TATACCG	175	4.7535964E-4	20.420298	5
ATCAACG	10530	0.0	20.305964	3
ACGCAGA	10640	0.0	19.801537	7
CGCAGAG	10775	0.0	19.663132	8
GGTATCA	7235	0.0	18.610842	1
ATGGGGG	3495	0.0	18.063736	5
GAGTACT	3245	0.0	17.693052	12-13
GTATAGG	610	0.0	17.580805	1
>>END_MODULE