FastQCFastQC Report
Mon 15 Aug 2016
SRR2920916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512144
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83440.33214656484660116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69380.27617843563107847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49450.1968438115012515No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT27010.1075177219140304No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25940.10325841193816913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA130450.035.6811331
GGTATCA94500.034.667991
TCAACGC160650.028.7635294
TATCAAC161100.028.7582552
ATCAACG161800.028.6694983
CAACGCA161200.028.5915095
AACGCAG163750.028.20276
CATGGGG63600.027.6201524
ACGCAGA181100.025.4274657
GAGTACT105250.025.27123612-13
AGTACTT108700.025.0986812-13
CGCAGAG185150.024.772898
TACATGG120850.024.1450392
GTACATG123000.023.7727321
ACATGGG118700.023.3272423
GCAGAGT192800.023.083939
GTACTTT115400.022.99694414-15
AGAGTAC170650.021.86197510-11
TAGGCCG1754.7600764E-420.4161745
TACTTTT132550.020.38061114-15
Produced by FastQC (version 0.11.5)