FastQCFastQC Report
Mon 15 Aug 2016
SRR2920915_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920915_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4653396
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92700.036.896061
GTACATG178050.033.4996261
GGTATCA66850.032.979811
TCAACGC104900.031.9089324
TACATGG184100.031.5456642
CATGGGG125650.031.3321694
CAACGCA107500.031.137185
ATCAACG108200.031.0485063
ACATGGG183700.030.9982813
AACGCAG110000.030.5187576
TATCAAC114850.029.302612
ACGCAGA124700.026.6321437
CGCAGAG129550.025.6326278
ATGGGGG68750.022.2644445
GCAGAGT149000.021.247729
GTATAGG11350.020.9996471
ATGGGGA61000.020.9923045
AGAGTAC130950.018.97155610-11
GTATAAT23750.018.8167881
CAGAGTA140800.018.44733210-11
Produced by FastQC (version 0.11.5)