FastQCFastQC Report
Mon 15 Aug 2016
SRR2920915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4653396
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85100.18287719334438762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70090.15062118074627648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86700.047.340451
GTATCAA136600.044.85211
ATCAACG170300.035.210233
TCAACGC170550.035.1237074
CAACGCA173550.034.4822435
TATCAAC176150.034.2110142
AACGCAG179950.033.4357076
GTACATG175950.030.1422421
CATGGGG112800.029.825894
ACGCAGA206300.028.7841767
TACATGG182800.028.3733122
CGCAGAG210450.028.2411948
ACATGGG177050.028.049443
GCAGAGT236800.024.4226679
GAGTACT134300.023.63663112-13
AGAGTAC216800.022.27161610-11
ATGGGGG63150.022.2532125
AGTACTT144550.022.1663812-13
CAGAGTA225950.021.3697110-11
GTACTTT151000.020.96340814-15
Produced by FastQC (version 0.11.5)