FastQCFastQC Report
Mon 15 Aug 2016
SRR2920914_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920914_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211547
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22610.10223612701877917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC1556.821854E-626.8776576
GTATCAA62100.026.7682271
CATGGGG55050.026.720224
TACATGG96450.025.7480342
GTACATG99150.025.5389611
ACATGGG98950.024.67623
CGTATAG1751.7333772E-523.8323551
GGTATCA48900.023.7592531
TCAACGC70550.022.9600224
AACGCAG70750.022.7964886
CAACGCA71800.022.6427275
TATATCG1602.5971598E-422.3317015
TATCAAC74100.022.1016522
ATCAACG74200.021.6705593
GTACCGT2858.032475E-820.8823916
TACCGTA2357.3849696E-620.2599037
GTATAGC8850.020.196911
ACGCAGA80400.019.8378227
CGCAGAG80200.019.7384348
TATGCCG2201.0152882E-418.9481125
Produced by FastQC (version 0.11.5)