##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920914_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2211547
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.529273852194866	31.0	30.0	34.0	24.0	34.0
2	29.548979515244305	31.0	30.0	34.0	24.0	34.0
3	29.709939693798052	31.0	30.0	34.0	25.0	34.0
4	33.08481664644704	37.0	35.0	37.0	26.0	37.0
5	33.19603110401904	37.0	35.0	37.0	27.0	37.0
6	33.1539090057774	37.0	35.0	37.0	26.0	37.0
7	33.07638227901103	37.0	35.0	37.0	26.0	37.0
8	32.994968228122666	37.0	35.0	37.0	26.0	37.0
9	34.390518040086874	39.0	35.0	39.0	25.0	39.0
10-11	34.3943605991643	39.0	35.0	39.0	25.0	39.0
12-13	34.33551740026326	39.0	35.0	39.0	25.0	39.0
14-15	35.46003340647972	40.0	36.0	41.0	25.0	41.0
16-17	35.439288199617735	40.0	36.0	41.0	25.0	41.0
18-19	35.417927134263934	40.0	36.0	41.0	25.0	41.0
20-21	35.44840715571498	40.0	36.0	41.0	25.0	41.0
22-23	35.360430730163095	40.0	35.0	41.0	25.0	41.0
24-25	35.361595525665976	40.0	35.0	41.0	25.0	41.0
26-27	35.29324065914041	40.0	35.0	41.0	25.0	41.0
28-29	35.18181978497405	40.0	35.0	41.0	24.0	41.0
30-31	35.03109904514803	40.0	35.0	41.0	24.0	41.0
32-33	34.90041518448398	39.0	35.0	41.0	24.0	41.0
34-35	34.82733444055225	39.0	34.5	41.0	23.5	41.0
36-37	34.67801973008035	39.0	34.0	41.0	22.5	41.0
38-39	34.537903331921044	39.0	34.0	41.0	21.0	41.0
40-41	34.40687355954904	39.0	34.0	41.0	19.5	41.0
42-43	34.197695775852836	39.0	33.5	41.0	18.5	41.0
44-45	34.02417425449244	39.0	33.0	41.0	17.5	41.0
46-47	33.812940443951675	38.0	33.0	40.5	15.0	41.0
48-49	33.67675070889292	38.0	33.0	40.0	14.0	41.0
50-51	33.473438728636566	38.0	33.0	40.0	10.5	41.0
52-53	33.27671670554594	38.0	32.5	40.0	8.5	41.0
54-55	33.033817956389804	38.0	32.0	40.0	8.0	41.0
56-57	32.76143780801403	37.5	32.0	40.0	7.0	41.0
58-59	32.50402704532166	37.0	31.5	40.0	4.0	41.0
60-61	32.22532666047793	37.0	31.0	40.0	2.0	41.0
62-63	31.907067315322713	36.0	31.0	39.5	2.0	41.0
64-65	31.62208942428083	36.0	31.0	39.0	2.0	41.0
66-67	31.269190299821798	35.0	30.0	39.0	2.0	40.5
68-69	30.939147800159798	35.0	30.0	38.5	2.0	40.0
70-71	30.596978947316064	35.0	30.0	38.0	2.0	40.0
72-73	30.659795609136953	35.0	30.0	37.0	2.0	39.5
74-75	30.6526838452902	35.0	31.0	37.0	2.0	39.0
76-77	30.39513449182857	35.0	31.0	36.5	2.0	39.0
78-79	30.112584765324904	35.0	30.0	36.0	2.0	39.0
80-81	29.844868998940562	35.0	30.0	36.0	2.0	37.0
82-83	29.610517208090084	35.0	30.0	35.0	2.0	37.0
84-85	29.378009827509885	35.0	30.0	35.0	2.0	36.5
86-87	29.18974885001314	35.0	30.0	35.0	2.0	36.0
88-89	28.997915260222822	34.5	30.0	35.0	2.0	36.0
90-91	28.83036783753635	34.0	29.5	35.0	2.0	36.0
92-93	28.668927226054883	34.0	29.0	35.0	2.0	35.0
94-95	28.559240658236067	34.0	29.0	35.0	2.0	35.0
96-97	28.46470344062324	34.0	29.0	35.0	2.0	35.0
98-99	28.328907095349997	34.0	29.0	35.0	2.0	35.0
100-101	28.19891799722095	34.0	29.0	35.0	2.0	35.0
102-103	26.84116209151332	32.5	26.0	34.5	2.0	35.0
104-105	27.854394457816177	34.0	28.5	35.0	2.0	35.0
106-107	28.00187402754723	34.0	29.0	35.0	2.0	35.0
108-109	27.925095193545513	34.0	29.0	35.0	2.0	35.0
110-111	27.81553071221186	34.0	29.0	35.0	2.0	35.0
112-113	27.68970250236599	34.0	28.5	35.0	2.0	35.0
114-115	27.564869523460274	34.0	27.5	35.0	2.0	35.0
116-117	27.338158763978335	34.0	27.0	35.0	2.0	35.0
118-119	27.12477781390131	34.0	27.0	35.0	2.0	35.0
120-121	26.93302403249852	34.0	26.5	35.0	2.0	35.0
122-123	26.625089360524555	34.0	25.0	35.0	2.0	35.0
124-125	25.336767656305746	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95436.0
3	13549.0
4	9130.0
5	8727.0
6	10977.0
7	11250.0
8	10879.0
9	11659.0
10	12542.0
11	13707.0
12	14064.0
13	13811.0
14	13753.0
15	12376.0
16	10102.0
17	9191.0
18	8837.0
19	8815.0
20	9070.0
21	9812.0
22	11816.0
23	15202.0
24	20342.0
25	25449.0
26	28905.0
27	32357.0
28	37701.0
29	43759.0
30	51769.0
31	62879.0
32	79149.0
33	100665.0
34	135226.0
35	197500.0
36	348159.0
37	535534.0
38	177255.0
39	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82784212943546	18.646555855195636	12.075500241413078	25.45010177395583
2	16.206794583425225	20.751913611582697	38.0130682134659	25.028223591526178
3	19.156960219230204	24.338855609281048	29.295904154729417	27.208280016759335
4	12.854551316769038	16.402736242258694	35.93980665530339	34.80290578566888
5	13.68577580857461	37.94763413284149	34.28068271480686	14.085907343777034
6	32.89669164478026	36.20509213112469	16.89825062671617	13.999965597378882
7	28.10632606787562	32.58320360648925	21.09157047868187	18.218899846953256
8	26.191604510460753	35.384884171972516	19.92827764508433	18.495233672482396
9	26.28069740261974	15.938115883119247	19.114703520599523	38.66648319366149
10-11	25.054989020299324	26.430778101426007	27.672760566491007	20.841472311783672
12-13	25.29949583604286	24.077415255790463	27.41597983887208	23.207109069294603
14-15	22.94832187157918	25.56143890908416	24.759809992972258	26.730429226364404
16-17	23.12957600970031	28.064646704164332	25.592190156105016	23.213587130030344
18-19	23.061170372830706	27.38555132253967	27.0354639647365	22.517814339893125
20-21	24.000009049743042	26.58676497704193	26.630927723095958	22.78229825011906
22-23	23.61896669593989	26.425734918521353	26.286675797016667	23.66862258852209
24-25	23.326406073184625	26.561540629896534	26.65354966655491	23.458503630363932
26-27	23.14023670084955	26.67285252851062	26.650272096468676	23.536638674171154
28-29	23.239252116544566	26.803621768621294	26.6667194577302	23.290406657103944
30-31	23.4545509253928	26.787670840834068	26.460779485095777	23.296998748677357
32-33	23.114955729866658	26.89337065726871	26.48888472569532	23.50278888716931
34-35	23.346422879525193	26.905397786150182	26.546785247879324	23.20139408644531
36-37	23.46711387134705	26.707115400844977	26.481175070722312	23.34459565708566
38-39	23.241252502024356	26.934870555378705	26.445155920207625	23.378721022389314
40-41	23.328100669868412	26.850057266667182	26.625787331825833	23.196054731638576
42-43	23.24774911243996	26.7821058483368	26.55609508426022	23.41404995496302
44-45	23.214975354122583	26.83091821758734	26.473860121099303	23.48024630719078
46-47	23.179436618252545	26.816344047877333	26.536080734295307	23.468138599574807
48-49	23.16133039973252	26.85667658860773	26.45371366988304	23.528279341776713
50-51	23.000666934526397	27.03392407262618	26.422484471375206	23.542924521472212
52-53	23.15535231472757	26.958297597390864	26.33019716738312	23.55615292049845
54-55	23.12650987564439	26.813196907014508	26.34205009608454	23.71824312125656
56-57	23.100738432662084	26.92627574095534	26.246011789401663	23.72697403698091
58-59	23.160165276729934	26.689952301398158	26.41597659974686	23.73390582212505
60-61	23.187377205993478	26.736604763253606	26.47237423314549	23.603643797607425
62-63	23.057669058081704	26.69867989546307	26.549638753954525	23.694012292500705
64-65	23.096200329599608	26.683180579638343	26.493881369026006	23.72673772173604
66-67	23.123783875865243	26.931517888067603	26.21523052883599	23.729467707231173
68-69	23.297820257927548	26.6094907057579	26.317238577099992	23.77545045921456
70-71	23.036534917633666	26.54974443338957	26.537618762575292	23.876101886401468
72-73	23.202421210338237	26.611619827991635	26.440285581207434	23.74567338046269
74-75	23.017028360693676	26.706992886921665	26.432224418831662	23.843754333552994
76-77	23.062591332240874	26.532260600648556	26.51877640419467	23.886371662915902
78-79	23.023609520907325	26.68375150697024	26.513219825020652	23.779419147101788
80-81	23.11852939073906	26.585907249435113	26.510415865452046	23.785147494373778
82-83	23.070261308895063	26.602477596454975	26.560895494767056	23.7663655998829
84-85	23.11017840494123	26.630337278707582	26.3581044195024	23.90137989684879
86-87	23.045418863861045	26.56298850106664	26.50387633726283	23.88771629780948
88-89	23.1113453766428	26.407944014559654	26.478072859345293	24.002637749452262
90-91	23.15881084331435	26.45658934703434	26.52692379650382	23.857676013147497
92-93	23.285451843620965	26.446041876528508	26.464071237920383	23.80443504193014
94-95	23.014702322426686	26.442732976811932	26.613701263178946	23.928863437582436
96-97	23.155248345460702	26.415805399893276	26.426188330987937	24.002757923658084
98-99	23.082592150150187	26.471544288781963	26.367466855714657	24.078396705353192
100-101	23.029311451647708	26.614581848895668	26.402541945401648	23.953564754054973
102-103	22.962633387559965	26.67527265620617	26.409017786214374	23.95307617001949
104-105	22.882948661661963	26.772551183057935	26.263169120951773	24.081331034328322
106-107	22.78023712428609	26.89214534568202	26.284166838025396	24.043450692006495
108-109	22.84384910387868	26.91193925825084	26.34981476291078	23.894396874959707
110-111	22.814907452255625	27.15969810818778	26.134509312689598	23.890885126867
112-113	22.865203173127284	27.273895451496465	26.138709505921053	23.7221918694552
114-115	22.905187561833042	27.418725392626392	25.994843183597734	23.681243861942836
116-117	22.80857188817242	27.406327128407508	26.043855824784846	23.741245158635227
118-119	22.93507580006276	27.27076111357578	25.991013624351762	23.803149462009703
120-121	22.900563029204353	27.50105934192526	25.910722983613628	23.68765464525676
122-123	22.92319623335908	27.65977614982587	25.82663352847943	23.59039408833562
124-125	23.207718227935338	27.666410771055784	25.578270910415945	23.54760009059293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2101.0
1	1542.0
2	810.0
3	589.5
4	452.5
5	361.0
6	341.5
7	369.0
8	438.5
9	475.0
10	501.5
11	562.0
12	587.5
13	603.5
14	684.0
15	774.0
16	888.0
17	1042.0
18	1218.5
19	1428.5
20	1771.5
21	2235.5
22	2825.5
23	3601.0
24	4459.0
25	5775.0
26	8135.5
27	10753.5
28	12541.0
29	14729.5
30	17891.0
31	21532.0
32	25979.5
33	30507.5
34	35227.0
35	39353.0
36	43344.5
37	47960.0
38	52098.0
39	55756.5
40	59322.5
41	62981.0
42	66577.0
43	71891.0
44	76766.0
45	80352.0
46	84159.5
47	86580.0
48	88612.5
49	91061.0
50	93652.0
51	92906.0
52	88797.0
53	87154.5
54	85632.5
55	80915.5
56	74446.5
57	67159.0
58	61372.0
59	55078.5
60	46176.0
61	37927.5
62	30729.5
63	22914.5
64	16494.5
65	12555.5
66	9368.0
67	6734.5
68	4918.0
69	3746.0
70	2960.0
71	2268.0
72	1714.0
73	1496.0
74	1281.5
75	993.0
76	705.5
77	493.5
78	354.0
79	241.5
80	195.0
81	131.5
82	103.0
83	86.0
84	61.0
85	49.0
86	34.5
87	23.0
88	18.0
89	17.5
90	17.0
91	12.0
92	10.5
93	11.0
94	6.5
95	4.5
96	8.0
97	10.0
98	14.0
99	14.0
100	22.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16789152570576163
2	0.14867420859696856
3	0.17282020232895795
4	0.14754377817880426
5	0.17060455870935592
6	0.10910914396121811
7	0.10933523004485095
8	0.09920657349809885
9	0.10494916002237346
10-11	0.09111269170404247
12-13	0.0791301292715009
14-15	0.07840665380387575
16-17	0.07779622137806702
18-19	0.07551275193337514
20-21	0.0696119051505575
22-23	0.06041020154670012
24-25	0.06502235765281046
26-27	0.07546753471664856
28-29	0.07130755077780396
30-31	0.06717017544732262
32-33	0.07709535451880516
34-35	0.07420145264830455
36-37	0.075580577758465
38-39	0.07173711433670639
40-41	0.07732144060243802
42-43	0.0767110081766293
44-45	0.06974755680073721
46-47	0.0670345237971429
48-49	0.05851107844418409
50-51	0.06488670600263075
52-53	0.06649191719642404
54-55	0.06845886612402992
56-57	0.07216667789560882
58-59	0.05943803138707882
60-61	0.06986059984255365
62-63	0.06416323053500558
64-65	0.05830760096891452
66-67	0.063326712025564
68-69	0.06642409137133418
70-71	0.061495414748137835
72-73	0.0564084778663985
74-75	0.0597997691208914
76-77	0.07019972896800294
78-79	0.063326712025564
80-81	0.06154063196486441
82-83	0.06608496224588489
84-85	0.06739626153095547
86-87	0.06068150484705955
88-89	0.060116289637977396
90-91	0.06296497429175144
92-93	0.05713195333402365
94-95	0.06728321848913905
96-97	0.05374066207953076
98-99	0.05306240382863218
100-101	0.050371979433401146
102-103	0.04842763911415855
104-105	0.046370255753099526
106-107	0.05749369106783622
108-109	0.05749369106783622
110-111	0.06409540470991573
112-113	0.06441192522700173
114-115	0.06613017946261147
116-117	0.07033538061818266
118-119	0.06436670801027516
120-121	0.06549713842843946
122-123	0.0636658411510133
124-125	0.0751284055911993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2211547.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.64708765074555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.77426098582968	36.55284460551888
2	14.061133681462055	14.524332074306423
3	5.950679005239129	9.220057204951098
4	3.1198284514771824	6.445202139549272
5	1.794699668057102	4.634550553145455
6	1.0665785495864304	3.3051405502137263
7	0.720765059589649	2.605779134575501
8	0.5212709176208178	2.153769981771755
9	0.35962697699119495	1.6716317402013208
>10	1.5311517160013361	13.3714810193573
>50	0.07006044769646572	2.452111074574214
>100	0.028892058943846475	2.4432873359396456
>500	6.139475447156672E-4	0.22119015251975002
>1k	4.385339605111508E-4	0.39862243337572467
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2261	0.10223612701877917	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.043443345314388E-5
2	0.0	0.0	0.0	0.0	9.043443345314388E-5
3	0.0	0.0	0.0	0.0	9.043443345314388E-5
4	0.0	0.0	0.0	0.0	9.043443345314388E-5
5	0.0	0.0	0.0	0.0	9.043443345314388E-5
6	0.0	0.0	0.0	0.0	9.043443345314388E-5
7	0.0	0.0	0.0	0.0	9.043443345314388E-5
8	0.0	0.0	0.0	0.0	1.3565165017971582E-4
9	0.0	0.0	0.0	0.0	1.3565165017971582E-4
10-11	0.0	0.0	0.0	4.521721672657194E-5	1.8086886690628777E-4
12-13	0.0	0.0	0.0	4.521721672657194E-5	3.165205170860036E-4
14-15	0.0	0.0	0.0	4.521721672657194E-5	3.6173773381257553E-4
16-17	0.0	0.0	0.0	6.782582508985791E-5	4.069549505391475E-4
18-19	0.0	0.0	0.0	1.3565165017971582E-4	4.5217216726571943E-4
20-21	0.0	0.0	0.0	2.486946919961457E-4	6.330410341720072E-4
22-23	0.0	0.0	0.0	4.973893839922913E-4	6.782582508985791E-4
24-25	0.0	0.0	0.0	8.139099010782949E-4	6.782582508985791E-4
26-27	0.0	0.0	0.0	0.001763471452336306	6.782582508985791E-4
28-29	0.0	0.0	0.0	0.00454433028102048	7.234754676251511E-4
30-31	0.0	0.0	0.0	0.0138590769266943	7.234754676251511E-4
32-33	0.0	0.0	0.0	0.03829898256740644	7.68692684351723E-4
34-35	0.0	0.0	0.0	0.08121012124092321	9.043443345314389E-4
36-37	0.0	0.0	0.0	0.15780808637573607	9.043443345314389E-4
38-39	0.0	0.0	0.0	0.2810250019556446	0.001017387376347869
40-41	0.0	0.0	0.0	0.425652269655585	0.0010399959847111548
42-43	0.0	0.0	0.0	0.5631126085043636	0.0010399959847111548
44-45	0.0	0.0	0.0	0.7295119660581484	0.0010626045930744407
46-47	0.0	0.0	0.0	0.9363355153654884	0.0011756476348908705
48-49	0.0	0.0	0.0	1.1478164379956655	0.0012886906767073003
50-51	0.0	0.0	0.0	1.3665773325188204	0.0013112992850705864
52-53	0.0	0.0	0.0	1.5850443151332527	0.0014017337185237302
54-55	2.260860836328597E-5	0.0	0.0	1.8335129210457657	0.001424342326887016
56-57	4.521721672657194E-5	0.0	0.0	2.1309969898898826	0.001492168151976874
58-59	4.521721672657194E-5	0.0	0.0	2.4544131325266885	0.001537385368703446
60-61	4.521721672657194E-5	0.0	0.0	2.787935323101883	0.001582602585430018
62-63	4.521721672657194E-5	0.0	0.0	3.1211409931599916	0.0017182542356097338
64-65	4.521721672657194E-5	0.0	0.0	3.4752822345625027	0.0017182542356097338
66-67	4.521721672657194E-5	0.0	0.0	3.8912354112302383	0.0017182542356097338
68-69	4.521721672657194E-5	0.0	0.0	4.366875313977049	0.0017182542356097338
70-71	4.521721672657194E-5	0.0	0.0	4.880045506606914	0.0017182542356097338
72-73	4.521721672657194E-5	0.0	0.0	5.3776835852912015	0.0017860800606995918
74-75	4.521721672657194E-5	0.0	0.0	5.889926824978172	0.0019217317108793075
76-77	4.521721672657194E-5	0.0	0.0	6.457493329330102	0.0020347747526957374
78-79	4.521721672657194E-5	0.0	0.0	7.091551750878457	0.0020347747526957374
80-81	4.521721672657194E-5	0.0	0.0	7.754617016956908	0.0020347747526957374
82-83	4.521721672657194E-5	0.0	0.0	8.443456096569506	0.0021252091861488813
84-85	4.521721672657194E-5	0.0	0.0	9.120629134266647	0.0021252091861488813
86-87	4.521721672657194E-5	0.0	0.0	9.83318464405233	0.002215643619602025
88-89	4.521721672657194E-5	0.0	0.0	10.62387550434153	0.002260860836328597
90-91	4.521721672657194E-5	0.0	0.0	11.462767917661257	0.002260860836328597
92-93	4.521721672657194E-5	0.0	0.0	12.309980298858672	0.002260860836328597
94-95	4.521721672657194E-5	0.0	0.0	13.157373548922994	0.0023060780530551692
96-97	4.521721672657194E-5	0.0	0.0	14.024594548521918	0.002419121094871599
98-99	4.521721672657194E-5	2.260860836328597E-5	0.0	14.955210085971494	0.002532164136688029
100-101	4.521721672657194E-5	4.521721672657194E-5	0.0	15.960976637620634	0.002532164136688029
102-103	4.521721672657194E-5	4.521721672657194E-5	0.0	16.977210974941976	0.0026225985701411724
104-105	4.521721672657194E-5	4.521721672657194E-5	0.0	17.972668001177457	0.0027130330035943164
106-107	4.521721672657194E-5	4.521721672657194E-5	0.0	18.978004989267696	0.0027130330035943164
108-109	4.521721672657194E-5	4.521721672657194E-5	0.0	20.014134901948726	0.0027130330035943164
110-111	4.521721672657194E-5	4.521721672657194E-5	0.0	21.109974149317196	0.0027582502203208886
112-113	4.521721672657194E-5	4.521721672657194E-5	0.0	22.231270689702725	0.0027582502203208886
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGC	155	6.821854E-6	26.877657	6
GTATCAA	6210	0.0	26.768227	1
CATGGGG	5505	0.0	26.72022	4
TACATGG	9645	0.0	25.748034	2
GTACATG	9915	0.0	25.538961	1
ACATGGG	9895	0.0	24.6762	3
CGTATAG	175	1.7333772E-5	23.832355	1
GGTATCA	4890	0.0	23.759253	1
TCAACGC	7055	0.0	22.960022	4
AACGCAG	7075	0.0	22.796488	6
CAACGCA	7180	0.0	22.642727	5
TATATCG	160	2.5971598E-4	22.331701	5
TATCAAC	7410	0.0	22.101652	2
ATCAACG	7420	0.0	21.670559	3
GTACCGT	285	8.032475E-8	20.882391	6
TACCGTA	235	7.3849696E-6	20.259903	7
GTATAGC	885	0.0	20.19691	1
ACGCAGA	8040	0.0	19.837822	7
CGCAGAG	8020	0.0	19.738434	8
TATGCCG	220	1.0152882E-4	18.948112	5
>>END_MODULE