FastQCFastQC Report
Mon 15 Aug 2016
SRR2920914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211547
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50400.2278947723019226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40680.18394363764369465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30200.13655599451424727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81700.036.7309271
GGTATCA56800.036.4474451
TCAACGC100800.028.8873524
TATCAAC103100.028.8224412
ATCAACG102750.028.455033
CAACGCA102900.028.3556825
AACGCAG105950.027.5806246
CATGGGG53200.026.5274894
TACATGG96250.024.5009752
ACGCAGA120050.024.1865047
GTACATG98550.024.0941931
CGCAGAG122800.023.6432658
ACATGGG96200.023.5835593
GAGTACT68400.022.79188712-13
GTATAGG5100.022.2264141
GCAGAGT132550.021.502119
AGTACTT73950.021.40319312-13
GTACTTT75550.020.6742614-15
AGAGTAC119150.020.3499710-11
GTTCGCG2951.1590055E-720.1694227
Produced by FastQC (version 0.11.5)