##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920914_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2211547
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.007060668391855	31.0	31.0	34.0	28.0	34.0
2	31.14039403187	33.0	31.0	34.0	27.0	34.0
3	31.226956062882678	33.0	31.0	34.0	27.0	34.0
4	34.762629507760856	37.0	35.0	37.0	32.0	37.0
5	34.45449361917246	37.0	35.0	37.0	30.0	37.0
6	34.16881305258265	37.0	35.0	37.0	30.0	37.0
7	34.0622446640293	37.0	35.0	37.0	30.0	37.0
8	34.05978891698888	37.0	35.0	37.0	30.0	37.0
9	35.589348994165626	39.0	35.0	39.0	30.0	39.0
10-11	35.55822666215097	39.0	35.0	39.0	30.0	39.0
12-13	35.44007588353311	39.0	35.0	39.0	29.5	39.0
14-15	36.639198262573665	40.0	36.0	41.0	30.0	41.0
16-17	36.64307383021929	40.0	36.5	41.0	30.0	41.0
18-19	36.69004977059045	40.0	37.0	41.0	30.0	41.0
20-21	36.68692752177549	40.0	37.0	41.0	30.0	41.0
22-23	36.629647708142755	40.0	37.0	41.0	29.5	41.0
24-25	36.6102780090136	40.0	37.0	41.0	30.0	41.0
26-27	36.47681622863995	40.0	36.0	41.0	29.0	41.0
28-29	36.37160254789973	40.0	36.0	41.0	29.0	41.0
30-31	36.22030822767954	40.0	36.0	41.0	28.5	41.0
32-33	36.06193741304164	40.0	35.5	41.0	28.0	41.0
34-35	35.93289516343085	40.0	35.0	41.0	27.0	41.0
36-37	35.794091647159206	40.0	35.0	41.0	27.0	41.0
38-39	35.671562259359625	40.0	35.0	41.0	26.5	41.0
40-41	35.53768335016167	40.0	35.0	41.0	26.0	41.0
42-43	35.389899694648136	40.0	35.0	41.0	25.0	41.0
44-45	35.18849542876548	39.5	35.0	41.0	24.5	41.0
46-47	34.96876575537395	39.0	35.0	41.0	23.5	41.0
48-49	34.712845352144896	39.0	34.5	41.0	23.0	41.0
50-51	34.5207036070226	39.0	34.0	41.0	22.0	41.0
52-53	34.322772475556704	39.0	34.0	41.0	21.5	41.0
54-55	34.11081315477356	38.5	34.0	41.0	20.0	41.0
56-57	33.85512788107148	38.0	33.0	40.5	18.5	41.0
58-59	33.633597658109906	38.0	33.0	40.0	18.0	41.0
60-61	33.364495531860726	37.0	33.0	40.0	16.0	41.0
62-63	33.06604064937349	37.0	33.0	40.0	13.5	41.0
64-65	32.75727714581693	36.5	32.0	40.0	12.5	41.0
66-67	32.42439297016975	36.0	32.0	39.0	9.0	41.0
68-69	32.049173497104064	35.5	32.0	39.0	7.5	41.0
70-71	31.71060687383085	35.0	31.5	38.5	6.5	40.0
72-73	31.34445933095702	35.0	31.0	37.5	3.5	40.0
74-75	31.01039114248985	35.0	31.0	37.0	2.0	39.0
76-77	30.79105101542043	35.0	31.0	36.5	2.0	39.0
78-79	30.6422312073856	35.0	31.0	36.0	2.0	39.0
80-81	30.385558841842386	35.0	31.0	36.0	2.0	37.0
82-83	30.157672886897725	35.0	31.0	35.0	2.0	37.0
84-85	29.910736014201824	35.0	31.0	35.0	2.0	36.5
86-87	29.738240471488965	35.0	31.0	35.0	2.0	36.0
88-89	29.57779147357031	35.0	30.5	35.0	2.0	36.0
90-91	29.412132095768257	35.0	30.0	35.0	2.0	36.0
92-93	29.292374975526183	34.0	30.0	35.0	2.0	35.0
94-95	29.195796200578148	34.0	30.0	35.0	2.0	35.0
96-97	29.126680554381164	34.0	30.0	35.0	2.0	35.0
98-99	29.020848980374375	34.0	30.0	35.0	2.0	35.0
100-101	28.914000923335564	34.0	30.0	35.0	2.0	35.0
102-103	27.505248362345455	32.5	26.0	34.0	2.0	35.0
104-105	28.56701869777129	34.0	29.0	35.0	2.0	35.0
106-107	28.740369071966366	34.0	29.0	35.0	2.0	35.0
108-109	28.836067467704734	34.0	29.5	35.0	2.0	35.0
110-111	29.007098650853905	35.0	30.0	35.0	2.0	35.0
112-113	28.974457698615495	35.0	30.0	35.0	2.0	35.0
114-115	28.882986886554978	35.0	30.0	35.0	2.0	35.0
116-117	28.765246454178907	35.0	30.0	35.0	2.0	35.0
118-119	28.65716238452088	35.0	30.0	35.0	2.0	35.0
120-121	28.46531161218821	35.0	30.0	35.0	2.0	35.0
122-123	28.25338665648978	35.0	30.0	35.0	2.0	35.0
124-125	27.49888471734944	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45256.0
3	18043.0
4	7465.0
5	6318.0
6	7483.0
7	8499.0
8	9557.0
9	10536.0
10	11897.0
11	13343.0
12	14201.0
13	15965.0
14	19337.0
15	17519.0
16	11418.0
17	10220.0
18	9597.0
19	9342.0
20	9330.0
21	9494.0
22	9797.0
23	10772.0
24	14425.0
25	19328.0
26	23906.0
27	28140.0
28	33657.0
29	40383.0
30	48471.0
31	58832.0
32	72949.0
33	93362.0
34	126132.0
35	186441.0
36	340731.0
37	601265.0
38	237595.0
39	541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92317082620215	19.048855453706334	12.72125923881792	25.306714481273595
2	16.625568482176895	21.032653498666665	37.30975037657634	25.0320276425801
3	19.10353789986878	24.345410298172585	29.971901907179525	26.579149894779107
4	13.304927132805092	16.570509733487256	35.843678173393585	34.28088496031407
5	13.599170204475627	37.67084324983335	34.472401469701104	14.25758507598992
6	32.98342243981185	36.394623841265144	16.54860454142173	14.073349177501282
7	27.93653378201127	32.44778239120013	21.130152785692964	18.485531041095644
8	26.514007546125384	34.894521122231765	19.901085428235607	18.690385903407243
9	26.520570792342042	14.900359164668545	19.32176577688014	39.25730426610927
10-11	25.310204668604	25.741357276005505	27.827907069292834	21.12053098609766
12-13	25.860051057440753	23.31409823017454	27.81499931034015	23.01085140204456
14-15	23.19514134920863	24.745524388343814	25.38460512155559	26.674729140891962
16-17	22.992183000156714	27.392855180280364	26.252224940180174	23.362736879382744
18-19	22.89522863614325	26.39283879324693	28.250552685090707	22.461379885519108
20-21	23.71528783562884	25.66186218903009	27.984776361255477	22.638073614085595
22-23	23.384936962229798	25.60050723267852	27.718438830434323	23.29611697465736
24-25	23.10082174905789	25.815270847964893	28.018156172569565	23.065751230407656
26-27	22.97074482638234	25.94390455788469	28.021243024926434	23.064107590806536
28-29	23.01082300417508	25.92849364331662	28.069736289896603	22.990947062611703
30-31	23.354989518611333	25.874004912493803	27.893698753290202	22.877306815604666
32-33	22.99926307532528	26.00281338349275	27.898647644859743	23.099275896322226
34-35	23.162644104682037	25.993429672211736	28.04000899078445	22.80391723232178
36-37	23.38993652097304	25.760043308290264	27.862186833483786	22.98783333725291
38-39	23.138626791418154	25.98173954829409	27.80380473188795	23.075828928399808
40-41	23.16577650878708	25.89922981631646	27.977332746578405	22.957660928318056
42-43	23.19427446702468	25.767951198465184	27.820185448003194	23.21758888650694
44-45	23.158665962645415	25.846662814901585	27.66593211772833	23.32873910472467
46-47	23.221605085954604	25.890899091813203	27.503528772213397	23.383967050018793
48-49	23.22809281993034	26.016200229609908	27.299587113714797	23.456119836744957
50-51	23.059160422719167	26.27586369079707	27.18703984428421	23.477936042199556
52-53	23.144884754489244	26.251714239522084	27.128455188959222	23.47494581702945
54-55	23.04716984971985	26.247559513499162	27.07294934687448	23.632321289906503
56-57	23.04163899334461	26.339417054545443	26.87791333088655	23.7410306212234
58-59	22.9252759473497	26.194562073992532	27.15697742650845	23.723184552149316
60-61	23.045250832331384	26.308862726546433	27.054574662437414	23.591311778684766
62-63	22.76634954362822	26.331026881169656	27.179115605967784	23.72350796923434
64-65	22.76183840956213	26.232514954393427	27.15507094871219	23.850575687332256
66-67	22.795173998768465	26.442602646824643	26.90027812395342	23.861945230453475
68-69	22.95835509758298	26.112599748100006	26.92609652246862	24.00294863184839
70-71	22.701451443529198	25.99153030647013	27.12725976832259	24.179758481678093
72-73	22.90187334352471	26.04431438883411	26.951927164837947	24.10188510280323
74-75	22.755138912191793	26.15186569206007	26.811434608181827	24.281560787566303
76-77	22.74504652009939	25.99027201149034	26.96664350478926	24.298037963621006
78-79	22.715376537531647	26.11804860223573	26.848633350428326	24.317941509804296
80-81	22.73693241279004	26.023558696393472	26.775495012115826	24.464013878700662
82-83	22.611259807498413	26.11721084117635	26.77283415364446	24.49869519768077
84-85	22.62092014634786	26.107400154669246	26.58805067000728	24.68362902897561
86-87	22.582708018263784	26.005561806696235	26.668700297401536	24.743029877638445
88-89	22.534509316905023	25.95636101164014	26.64378859431009	24.865341077144752
90-91	22.54159504463743	25.908090451556827	26.670913112472928	24.87940139133282
92-93	22.590279172161292	25.92049662363222	26.58545927827312	24.903764925933363
94-95	22.350414227782544	25.856053414784487	26.725346109370246	25.068186248062723
96-97	22.45430266237007	25.76855666804525	26.605520560834094	25.171620108750588
98-99	22.438060911387023	25.784682384810363	26.45661154688874	25.32064515691387
100-101	22.29643495470202	25.836357419896377	26.516324640365855	25.350882985035746
102-103	22.207560751068346	25.758584140599964	26.524478287190405	25.509376821141284
104-105	22.128989356590726	25.87696261387462	26.411631530430142	25.582416499104514
106-107	22.08814437989645	25.970324603338078	26.391445447271032	25.550085569494435
108-109	22.071738696380773	25.99170452497518	26.37945681665499	25.55709996198906
110-111	21.972372833565217	26.143310244744466	26.196989913694217	25.687327007996103
112-113	21.894333748922023	26.34961183835364	26.102067497210946	25.6539869155134
114-115	21.894506979327012	26.4357498548423	26.034734745779573	25.63500842005112
116-117	21.776018547136456	26.430551225182253	26.07528601754553	25.718144210135762
118-119	21.77680236438518	26.290920485972315	26.05438248705394	25.877894662588563
120-121	21.74978430773182	26.486739221264997	25.939821404993207	25.82365506600997
122-123	21.626621888683424	26.603643177593884	25.776897933916903	25.99283699980579
124-125	21.547840694179264	26.62909091320159	25.710971916532994	26.112096476086155
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	177.0
1	139.5
2	224.5
3	416.5
4	327.5
5	201.5
6	341.5
7	772.0
8	1235.0
9	1324.5
10	1073.0
11	817.0
12	786.5
13	869.0
14	980.5
15	1092.5
16	1202.5
17	1379.0
18	1541.5
19	1713.0
20	1967.0
21	2349.0
22	2914.5
23	3594.5
24	4358.0
25	5515.0
26	7611.0
27	10340.0
28	12311.5
29	14439.5
30	17670.5
31	21482.0
32	25945.5
33	30435.0
34	35109.0
35	39183.0
36	42968.5
37	47258.0
38	51824.0
39	55689.0
40	58978.5
41	62374.0
42	65872.5
43	71183.5
44	76009.0
45	80270.5
46	84671.0
47	87013.0
48	89070.5
49	91266.5
50	94030.5
51	93748.5
52	89926.0
53	88452.5
54	86508.0
55	81806.0
56	75397.0
57	68164.0
58	62268.0
59	55674.5
60	46991.5
61	38319.0
62	30416.5
63	22400.5
64	15829.0
65	11850.5
66	8637.5
67	6144.0
68	4483.0
69	3390.0
70	2678.5
71	2000.0
72	1484.0
73	1298.0
74	1090.0
75	811.5
76	559.0
77	339.5
78	224.0
79	136.0
80	85.5
81	58.0
82	34.0
83	23.0
84	14.5
85	9.0
86	8.5
87	7.5
88	4.5
89	6.0
90	4.0
91	3.5
92	5.0
93	5.0
94	5.5
95	7.0
96	6.0
97	2.0
98	1.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21206874644762239
2	0.007958230143876662
3	4.069549505391475E-4
4	0.0026225985701411724
5	0.08347098207725182
6	0.057425865242746364
7	0.027446850553029173
8	0.015645156987393893
9	0.013791251101604445
10-11	0.011982562432541565
12-13	0.014107771618690446
14-15	0.021726872637117818
16-17	0.02407816790689956
18-19	0.023648604347997124
20-21	0.017205150964460625
22-23	0.015057333169948457
24-25	0.01302255841725272
26-27	0.012660820683440144
28-29	0.014966898736495314
30-31	0.011146043923099985
32-33	0.014944290128132028
34-35	0.017815583390269344
36-37	0.019126882675339935
38-39	0.021907741504024105
40-41	0.02333208383091112
42-43	0.02120687464476224
44-45	0.01987296675132837
46-47	0.019805140926238512
48-49	0.018335581382624925
50-51	0.01532863647030789
52-53	0.013090384242342578
54-55	0.018539058857894496
56-57	0.021817307070570963
58-59	0.017702540348452915
60-61	0.019827749534601796
62-63	0.026022508226142153
64-65	0.018426015816078067
66-67	0.021817307070570963
68-69	0.015916460287753325
70-71	0.016685152972105047
72-73	0.023829473214903415
74-75	0.014921681519768741
76-77	0.013497339192881723
78-79	0.016866021839011335
80-81	0.017589497306636486
82-83	0.013226035892522294
84-85	0.01704689070591762
86-87	0.01786080060699592
88-89	0.018312972774261638
90-91	0.012932123983799577
92-93	0.013339078934338722
94-95	0.014650378219409309
96-97	0.015396462295397748
98-99	0.013655599451424728
100-101	0.01858427607462107
102-103	0.024259036773805847
104-105	0.008071273185693091
106-107	0.008433010919505667
108-109	0.015441679512124317
110-111	0.012728646508530003
112-113	0.010219090980205259
114-115	0.007189537459524939
116-117	0.00454433028102048
118-119	0.002848684653774032
120-121	0.002848684653774032
122-123	0.0019669489276058793
124-125	6.556496425352931E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2211547.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.741692129423555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.50891874667242	34.57491236546097
2	14.631537236040707	14.555948411506675
3	6.212535368389416	9.270660649127437
4	3.1450844681203134	6.257672933370897
5	1.8833102057861268	4.683951822020742
6	1.1558867711312935	3.449745834365177
7	0.7579487913465546	2.6391158800320307
8	0.5524359236379254	2.198327810786514
9	0.3811924982181296	1.7065043899570833
>10	1.6582994346265076	13.953709575335104
>50	0.07938061830507205	2.71617513420956
>100	0.03192527587638151	2.778347379230973
>500	8.177621554785782E-4	0.2899357140968929
>1k	6.360372320388149E-4	0.6972016096115774
>5k	9.08624617198307E-5	0.22779049088826336
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5040	0.2278947723019226	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4068	0.18394363764369465	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3020	0.13655599451424727	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.521721672657194E-5
6	0.0	0.0	0.0	0.0	9.043443345314388E-5
7	0.0	0.0	0.0	0.0	9.043443345314388E-5
8	0.0	0.0	0.0	0.0	9.043443345314388E-5
9	0.0	0.0	0.0	0.0	9.043443345314388E-5
10-11	0.0	0.0	0.0	4.521721672657194E-5	9.043443345314388E-5
12-13	0.0	0.0	0.0	4.521721672657194E-5	5.199979923555774E-4
14-15	0.0	0.0	0.0	4.521721672657194E-5	6.556496425352931E-4
16-17	0.0	0.0	0.0	6.782582508985791E-5	6.782582508985791E-4
18-19	0.0	0.0	0.0	1.3565165017971582E-4	6.782582508985791E-4
20-21	0.0	0.0	0.0	2.2608608363285971E-4	9.043443345314389E-4
22-23	0.0	0.0	0.0	4.5217216726571943E-4	9.495615512580108E-4
24-25	0.0	0.0	0.0	7.68692684351723E-4	9.495615512580108E-4
26-27	0.0	0.0	0.0	0.0017408628439730199	9.495615512580108E-4
28-29	0.0	0.0	0.0	0.00454433028102048	9.495615512580108E-4
30-31	0.0	0.0	0.0	0.013723425276514585	9.495615512580108E-4
32-33	0.0	0.0	0.0	0.038118113700500146	9.495615512580108E-4
34-35	0.0	0.0	0.0	0.08012490803948549	0.0010399959847111548
36-37	0.0	0.0	0.0	0.15556983414777076	0.0010399959847111548
38-39	0.0	0.0	0.0	0.2758928478571787	0.0010399959847111548
40-41	0.0	0.0	0.0	0.41882446992987266	0.0010399959847111548
42-43	0.0	0.0	0.0	0.5542500340259555	0.0010852132014377265
44-45	0.0	0.0	0.0	0.718411139351775	0.0011982562432541564
46-47	0.0	0.0	0.0	0.9246920820583963	0.0013112992850705862
48-49	0.0	0.0	0.0	1.135517355046038	0.00151477676034016
50-51	0.0	0.0	0.0	1.3532834708012085	0.0016730370188831618
52-53	0.0	0.0	0.0	1.569873938921488	0.0016730370188831618
54-55	0.0	0.0	0.0	1.8168277680736606	0.0016730370188831618
56-57	0.0	0.0	0.0	2.1122318449483553	0.0016730370188831618
58-59	0.0	0.0	0.0	2.4328897373648397	0.0016730370188831618
60-61	0.0	0.0	0.0	2.764151067103706	0.0016730370188831618
62-63	0.0	0.0	0.0	3.09636195839383	0.0016730370188831618
64-65	0.0	0.0	0.0	3.449124074686181	0.0017634714523363057
66-67	0.0	0.0	0.0	3.862432044175412	0.0018312972774261636
68-69	4.521721672657194E-5	0.0	0.0	4.334658047059366	0.0018539058857894497
70-71	4.521721672657194E-5	0.0	0.0	4.8415430465642375	0.0018765144941527358
72-73	4.521721672657194E-5	0.0	0.0	5.3339811453249695	0.0019895575359691656
74-75	4.521721672657194E-5	0.0	0.0	5.83948701971968	0.0021252091861488813
76-77	4.521721672657194E-5	0.0	0.0	6.402509193790591	0.002170426402875453
78-79	4.521721672657194E-5	0.0	0.0	7.031751981757566	0.0021930350112387394
80-81	4.521721672657194E-5	0.0	0.0	7.689707702345914	0.0022156436196020253
82-83	4.521721672657194E-5	0.0	0.0	8.372781586825873	0.002260860836328597
84-85	4.521721672657194E-5	0.0	0.0	9.046472898835068	0.002260860836328597
86-87	4.521721672657194E-5	0.0	0.0	9.75486842468191	0.0023286866614184547
88-89	4.521721672657194E-5	0.0	0.0	10.54013321896392	0.0023965124865083128
90-91	4.521721672657194E-5	0.0	0.0	11.371406531265219	0.0023965124865083128
92-93	4.521721672657194E-5	0.0	0.0	12.212944151763448	0.002441729703234885
94-95	4.521721672657194E-5	0.0	0.0	13.053554819318785	0.002441729703234885
96-97	4.521721672657194E-5	0.0	0.0	13.91473932048471	0.0027582502203208886
98-99	4.521721672657194E-5	0.0	0.0	14.838843578725662	0.0029391190872271765
100-101	4.521721672657194E-5	4.521721672657194E-5	0.0	15.840224060352323	0.0029391190872271765
102-103	4.521721672657194E-5	4.521721672657194E-5	0.0	16.852343630951545	0.002984336303953748
104-105	4.521721672657194E-5	4.521721672657194E-5	0.0	17.846760661202318	0.00302955352068032
106-107	4.521721672657194E-5	4.521721672657194E-5	0.0	18.85291155919363	0.00302955352068032
108-109	4.521721672657194E-5	4.521721672657194E-5	0.0	19.892771892254608	0.003074770737406892
110-111	4.521721672657194E-5	4.521721672657194E-5	0.0	20.99324590433755	0.003074770737406892
112-113	4.521721672657194E-5	4.521721672657194E-5	0.0	22.12297545564259	0.003074770737406892
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8170	0.0	36.730927	1
GGTATCA	5680	0.0	36.447445	1
TCAACGC	10080	0.0	28.887352	4
TATCAAC	10310	0.0	28.822441	2
ATCAACG	10275	0.0	28.45503	3
CAACGCA	10290	0.0	28.355682	5
AACGCAG	10595	0.0	27.580624	6
CATGGGG	5320	0.0	26.527489	4
TACATGG	9625	0.0	24.500975	2
ACGCAGA	12005	0.0	24.186504	7
GTACATG	9855	0.0	24.094193	1
CGCAGAG	12280	0.0	23.643265	8
ACATGGG	9620	0.0	23.583559	3
GAGTACT	6840	0.0	22.791887	12-13
GTATAGG	510	0.0	22.226414	1
GCAGAGT	13255	0.0	21.50211	9
AGTACTT	7395	0.0	21.403193	12-13
GTACTTT	7555	0.0	20.67426	14-15
AGAGTAC	11915	0.0	20.34997	10-11
GTTCGCG	295	1.1590055E-7	20.169422	7
>>END_MODULE