FastQCFastQC Report
Mon 15 Aug 2016
SRR2920913_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920913_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857492
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80100.029.1596851
CATGGGG63850.028.726524
GTACATG123150.028.642871
TACATGG122400.027.7352642
ACATGGG124050.026.3581223
TCAACGC88700.025.042544
AACGCAG90400.024.8211886
ATCAACG90650.024.7035263
CAACGCA90400.024.703365
TATCAAC95300.023.6231462
GGTATCA62950.022.527371
ACGCAGA100500.022.1463387
CGCAGAG101400.021.8312458
TATTCCG2801.554883E-619.1415775
GCAGAGT110000.018.8791949
ATGGGGA36400.018.814375
GTATAAG9900.018.055741
GAGTACT32900.017.9940312-13
GTATAGG5650.017.9279141
CTATACT10450.017.6660164
Produced by FastQC (version 0.11.5)