FastQCFastQC Report
Mon 15 Aug 2016
SRR2920913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857492
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.1766234166184892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41740.14607214998327206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28580.10001777782754948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91200.035.130471
GGTATCA66100.034.479931
TCAACGC109600.028.7401584
ATCAACG110300.028.5582623
CAACGCA111500.028.1970145
TATCAAC114350.027.8071652
CATGGGG64750.027.4963744
AACGCAG115350.027.344766
GTACATG116050.026.8881571
TACATGG118100.025.9662342
ACATGGG118200.025.1886063
ACGCAGA130500.023.982387
CGCAGAG133700.023.4059228
GAGTACT71550.022.99457412-13
TATACTG13250.022.4697465
AGTACTT78950.020.99002312-13
GCAGAGT144900.020.7363559
GTACTTT82850.020.07377814-15
AGAGTAC132350.019.24241810-11
TTACGCG958.331617E-418.78799680-81
Produced by FastQC (version 0.11.5)