FastQCFastQC Report
Mon 15 Aug 2016
SRR2920912_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920912_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424070
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27760.11451814510307047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95250.028.4695721
GTATCAA68250.028.3801841
CATGGGG58550.027.7727494
TACATGG98650.026.9312672
ACATGGG103300.025.1999783
AACGCAG75050.024.3491426
TCAACGC77750.023.672344
GTATAGG5300.023.6144561
CAACGCA78200.023.4599515
TATCAAC82250.023.2481372
ATCAACG81550.022.7172343
GGTATCA53050.022.6934471
ACGCAGA83850.021.5789687
GGATCGT3358.8584784E-1021.3222226
CGCAGAG86550.020.6298648
AGTATCG2701.0950698E-619.8546335
ATGGGGA28400.019.505085
CCGTCTA3402.4379915E-819.2551549
GAGTACT29300.018.27738212-13
GCAGAGT95400.018.0294599
Produced by FastQC (version 0.11.5)