FastQCFastQC Report
Mon 15 Aug 2016
SRR2920912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424070
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59400.24504242864273723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50710.2091936288968553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35560.1466954337127228No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT27000.11138292211033508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65050.036.3305361
GTATCAA90900.036.175261
TCAACGC110650.029.279564
CAACGCA112150.028.9410535
ATCAACG113400.028.6745573
TATCAAC113550.028.6390422
AACGCAG115350.028.1723566
CATGGGG57600.026.6756064
GTACATG101950.026.342031
TACATGG103400.025.5749862
ACGCAGA130050.024.9807177
ACATGGG105050.024.3208263
CGCAGAG133750.024.2426748
GAGTACT77950.023.47208412-13
AGTACTT81000.022.95554512-13
GCAGAGT144150.021.8749969
AGAGTAC129500.020.76783810-11
GTACTTT88900.020.5475114-15
CAGAGTA136600.019.64483810-11
TTTACGC1908.2739233E-418.8067913
Produced by FastQC (version 0.11.5)