FastQCFastQC Report
Mon 15 Aug 2016
SRR2920911_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920911_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4235722
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84450.032.884381
GTACATG144300.030.808881
TCAACGC90250.029.5028064
TACATGG150550.029.2410092
CATGGGG104600.028.473534
CAACGCA93900.028.3559975
ACATGGG152400.028.0652033
AACGCAG95500.027.6741666
GGTATCA62050.027.6601241
ATCAACG97950.027.0635193
TATCAAC102550.026.0819072
ACGCAGA109300.024.06867
CGCAGAG111700.023.6022328
GTATAGG11400.018.8191971
GCAGAGT132500.018.7286859
TATACCG2952.5555491E-618.1728695
AGAGTAC111950.017.83238210-11
ATGGGGC44500.017.4014875
ATGGGGG58750.017.337695
ATGGGGA59350.017.062055
Produced by FastQC (version 0.11.5)